The metastable beta titanium alloy Ti-3Al-5Mo-7V-3Cr (Ti-3573) was used as experimental material in this paper. Different cold rolling reduction was conducted in this titanium alloy. The results show that the total elongation (A50) reached maximum value of 16% after 30% cold rolling. The tensile strength reached maximum value of 910 MPa when the cold reduction increases to 50%. Microstructural evaluation suggested that the precipitation of α phases, deformation twin and stress-induced martensite is responsible for the enhanced tensile properties. Moreover, the TRIP/ TWIP deformation mode contributes to the improvement of the ductility of the titanium alloys.