Microsporogenesis and crossing behavior of a tetraploid, interspecific inter-EBN hybrid potato

Panahandeh, Jaber; Valizadeh, M; Khosroshahly, M.; Yermishin, A. P.; Khoei, F Rahimzadeh; Mahna, Nasser

doi:10.1016/j.scienta.2008.02.006

Cited by 9 publications

(6 citation statements)

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“…Irregular meiosis and micronuclei have also been observed in S. ruiz‐lealli , an Argentinian species of hybrid origin, with very low pollen viability and also difficult to reproduce from seeds (Raimondi 2002; Marfil et al. 2009) and in S. stoloniferum × S. tuberosum artificial hybrids (Panahandeh et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

Meiotic abnormalities underlying pollen sterility in wild potato hybrids and spontaneous populations

et al. 2011

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Wild potato species are widely distributed in the Americas, where they spontaneously grow in very diverse habitats. These species - with low chromosome differentiation - form polyploid series with 2n = 2x, 3x, 4x and 6x (x =12). They are isolated in nature by external and internal hybridisation barriers that can be incomplete, allowing hybridisation in areas of sympatry. Nevertheless, most accessions in germplasm banks, regardless of genetic background of the sampled spontaneous populations, have been assigned specific categories based on morphological characters. To further investigate the extent of hybridisation in the group and for comparative purposes, pollen viability was estimated in (i) artificial hybrids between a commercial cultivar (Calén INTA) of the common potato (tetraploid Solanum tuberosum ssp. tuberosum) and the tetraploid cytotype of the related wild species S. gourlayi, and (ii) samples of plants (accessions) and inflorescences of natural populations from Argentina, tentatively classified as 'presumed hybrids' (S. infundibuliforme-S. gourlayi) and 'species' (S. infundibuliforme, S. gourlayi and S. chacoense). Regardless of origin, 98 out of 103 plants analysed had zero to 70% pollen viability (zero to 40% in eight of them). Pollen grains were of variable size and morphology and, in mostly male sterile plants, the only viable pollen grains were 2n and/or 4n. Furthermore, male sterile plants shared various abnormalities in meiosis I and II (unpaired chromosomes, unequal chromosome distribution, precocious/lagging chromosomes, parallel, tripolar, fused and multiple spindles, unequal size nuclei, dyads, triads and pentads in addition to normal tetrads, among others). These results provide novel evidence to support field observations of early potato botanists on the extent of spontaneous hybridisation in wild Argentinian potato populations, which is not reflected in the current taxonomy and has significant consequences for germplasm conservation and breeding.

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Section: Discussionmentioning

confidence: 99%

Meiotic abnormalities underlying pollen sterility in wild potato hybrids and spontaneous populations

et al. 2011

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“…В отличие от обычно используемых прямых скрещиваний между диким аллотетраплоидным видом S. stoloniferum (2n=4x, EBN=2) и культурным картофелем (2n=4х, EBN=4) в сочетании с культурой in vitro незрелых зародышей или семян (Iwanaga et al, 1991;Singsit, Hanneman, 1991;Watanabe et al, 1992;Janssen et al, 1997;Panahandeh et al, 2008), гибрид IGC 16/36.1 (2n=4х, предполагаемый геномный состав АААВ) был получен путем опыления митотически удвоенного триплоидного межвидового гибрида (2n=6х, EBN=4) пыльцой высоко фертильной диплоидной линии IGC 10.21 S. tuberosum (2n=2х, EBN=2). Объем скрещиваний (30 опыленных цветков) был значительно меньшим в сравнении с рассмотренным выше методом получения подобных гибридов (несколько сотен опыленных цветков), культура in vitro незрелых зародышей или семян не применялась.…”

Section: Discussionunclassified

“…При небольшом объеме скрещиваний удалось получить достаточно большое количество семян от опыления их сортами картофеля (в общей сложности 585 семян), что позволяет рассчитывать на успех дальнейшего беккросса и возможность изучения особенностей интрогрессии генов дикого вида при использовании схемы 3. В литературе описаны примеры успешного беккроссировании сортами картофеля тетраплоидных межвидовых гибридов с S. fendlery (= S. stoloniferum) (эффективность 1,33 семян/опыление; Janssen et al, 1997) и S. stoloniferum (25-26 семян/опыление, Panahandeh et al, 2008).…”

Section: Discussionunclassified

“…Для повышения вероятности спаривания хромосом субгенома В S. stoloniferum и генома А S. tuberosum, в настоящей работе предлагается получать тетраплоидные межвидовые гибриды (АААВ), у которых вероятность гомеологичной рекомбинации может быть выше, чем у пентаплоидных гибридов с геномным составом ААААВ (Adiwilaga, Brown, 1991;Watanabe et al, 1992;Bamberg et al, 1994;Spooner et al, 2008). В ряде работ удалось получить тетраплоидные гибриды между аллотетраплоидными дикими видами и культурным картофелем благодаря большому объему скрещиваний и использованию приема «двойного опыления» (rescue pollination) в сочетании с культурой in vitro незрелых зародышей или семян (Iwanaga et al, 1991;Singsit, Hanneman, 1991;Watanabe et al, 1992;Janssen et al, 1997;Panahandeh et al, 2008). Следует заметить, что эффективность гибридизации между аллотетраплоидными видами и сортами культурного картофеля очень низкая, прежде всего, из-за различий их эффективной плоидности (EBN).…”

Section: Introductionunclassified

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Development of chromosome-specific markers for a study on introgressive hybridization of potato with the wild Mexican allotetraploid species Solanum stoloniferum Schltdl

Antonova

Yermishin

Levy

et al. 2020

Biotehnologiâ i selekciâ rastenij

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In order to involve valuable germplasm of the wild Mexican allotetraploid potato species Solanum stoloniferum Schltdl. (genomic composition ААВВ) into breeding, pentaploid interspecific hybrids (ААAAВ) with cultivated potato S. tuberosum L. (АААА) and their backcross progenies are usually used. Homologous synapsis in meiosis of such hybrids is expected only between chromosomes of the A subgenome, therefore a question arose about a possibility of introgressing genetic material of the subgenome B into the A genome of cultivated potato. In this connection, development of various schemes for the B subgenome introgression into the genome of cultivated potato is considered as a topical issue. The previous research has yielded four schemes of S. stoloniferum involvement into breeding, which imply backcrossing with cultivated potato of the following interspecific hybrids: (1) hexaploids (genomic composition ААААВВ, the conventional introgression scheme), (2) tetraploids (putatively, АААВ), (3) self-pollination progeny of a 4x hybrid and (4) pentaploid hybrids with a putative genome composition of АААВВ. The present paper presents the first results of the development of chromosome-specific DNA markers for the identification of S. stoloniferum chromosomes in interspecific hybrids. An S. stoloniferum accession PI 205522 with a high degree of resistance to late blight and PVY had been found to possess several DNA-markers of the R-genes conferring resistance to these pathogens and was used in hybridization as a promising parent. A set of 23 SSR- and CAPS markers with the known chromosome location in S. tuberosum was generated. These markers detect polymorphism between parent genotypes, i.e., the diploid clone IGC 10/1.21 of cultivated potatoes S. tuberosum, and accession PI 205522 of S. stoloniferum. All the markers specific for the wild species were found in triploid (ААВ) and pentaploid (АААВВ) hybrids of S. stoloniferum × S. tuberosum. This set of markers will be used for efficiency assessment of different schemes for S. stoloniferum genetic material introgression into the obtained BC2-BC3 generations after crossing the interspecific hybrids with cultivated potato.

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“…Fenomena di atas umumnya telah dilaporkan oleh banyak peneliti atau kelompok penelit pada berbagai tanaman budidya. Tanaman-tanaman dimaksud seperti tanaman tembakau (Volcov et al, 2005), Brassica (Zhang et al, 2006), kedelai (Rodrigues et al, 2006), tomat (Segui-Semarro dan Nuez, 2007), dan kentang (Panahandeh et al, 2008). Bagi tanaman jarak pagar, selain morfologi bunga berupa panjang dan lebar bunga, umur bunga juga menentukan frekuensi mikrospora uninukleat tengah dan akhir.…”

Section: E F Pembahasanunclassified

MORFOLOGI BUNGA YANG SESUAI BAGI KULTUR MIKROSPORA PADA TANAMAN JARAK PAGAR (Jatropha curcas L.)

Suaib¹,

Arma²,

Muhidin³

2015

Agroteknos

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An experiment aimed in gaining information on morphological characteristics of Jatropha curcas (L.) containing high frequency of uninucleate microspores has been done. This was important due to the successful application of experiment in haploid technique through anther or microspore culture is strongly determined by the availability of information from basic research such as appropriate morphology of the flowers. This scientific article, in our knowledge, was an early explanation of the initial steps of whole procedures in the microspore culture of Jatropha curcas (L.). Results of the research showed that the morphological features of flower that contained high frequency of mid- and late uninucleate microspores was that the flowers must be in the range of 2 to 3 mm in length. Such flowers contained yellowish anthers.Key words: Flowers length, mid- and late-uninucleate, morphology, physic nut, microspore

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Microsporogenesis and crossing behavior of a tetraploid, interspecific inter-EBN hybrid potato

Cited by 9 publications

References 20 publications

Meiotic abnormalities underlying pollen sterility in wild potato hybrids and spontaneous populations

Meiotic abnormalities underlying pollen sterility in wild potato hybrids and spontaneous populations

Development of chromosome-specific markers for a study on introgressive hybridization of potato with the wild Mexican allotetraploid species Solanum stoloniferum Schltdl

MORFOLOGI BUNGA YANG SESUAI BAGI KULTUR MIKROSPORA PADA TANAMAN JARAK PAGAR (Jatropha curcas L.)

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