Microsphere packages of carotenoids: intact sea urchin eggs tracked by Raman spectroscopy tools

Abstract

The native carotenoid content and distribution in intact, freshly released eggs of sea urchin P. lividus are described using resonance Raman spectroscopy; NIR-Raman revealed the additional molecular carotenoid content.

Application of Raman spectroscopy to the evaluation of F-actin changes in sea urchin eggs at fertilization

“…Moreover, the egg spectra also contained the characteristic bands typically associated with carotenoids, i.e. the peaks at 1150 cm −1 and 1508 cm −1 (Nekvapil et al, 2019). The higher frequency region was characterized by the two bands between 2895 and 2930 cm −1 Figure 2.…”

“…When a morphological change occurs in a selected cell or tissue during a biological process such as cell division, apoptosis, or stress response, this change can be identified, quantified, and visualized thanks to the consequent variation of the Raman spectrum (Short et al, 2005;Brauchle et al, 2014). Moreover, Raman spectroscopy has been used for studying the biochemical composition of male gametes of different mammalian species (Movasaghi et al, 2007;Ferrara et al, 2015a;De Angelis et al, 2019;Bogliolo et al, 2020) and for investigating the effect of vitrification/ warming on the state of the actin cytoskeleton of sheep oocytes matured in vitro (Bogliolo et al, 2015), as well as for monitoring the F-actin changes in sea urchin eggs at fertilization beta-carotene granules content of sea urchin eggs (Nekvapil et al, 2019). Raman spectroscopy can be applied to fixed and unfixed cells, providing biochemical information comparable and complementary to fluorescence microscopy.…”