Microspectrophotometric Study of Histoenzymic Reactions in Rat Epidermis Subjected to 0·5 Mrads of Deuteron Radiation

Cabrini, R. L.; Itoiz, M. E.; J, Mayo; Smolko, Eduardo E.; Klein‐Szanto, Andres J.

doi:10.1080/09553007014551301

Cited by 10 publications

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“…In particular, this study shows the potential value of AgNOR in the evaluation of the development of post-irradiation mucositis, a relatively frequent lesion of the oral cavity. AgNOR appear to be more sensitive than histological parameters and easier to demonstrate and evaluate than other markers, such as enzyme activity, tested in similar models (Cabrini et al, 1970;Cabrini, 1970, 1972;Itoiz et al, 1974;Itoiz and Frasch, 1977;Schwint et al, 1989). Variations in AgNOR are not specific to a particular lesion but reveal, with great sensitivity, changes in cellular activity associated with different entities.…”

Section: Discussionmentioning

confidence: 96%

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

et al. 2000

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The possibility of detection of incipient cellular alterations is central to early diagnosis and to clinician's capacity to discriminate between samples that appear similar on routine preparations. We examined the value of silver-stained nucleolar organizer regions (AgNOR) in detecting radio-induced alterations in a model of squamous epithelium biologically similar to oral mucosa. Morphometry of AgNOR has been proven to be of value in the detection of incipient cellular alterations. This method allows for the quantitative evaluation of lesions induced by high doses of radiation long before they become apparent in routine preparations. We herein examine the capacity of AgNOR to reveal the response to low doses of radiation, closer to the therapeutic or accidental dose to which the epithelium of oral mucosa may be exposed.

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Section: Discussionmentioning

confidence: 96%

AgNOR are Sensitive Markers of Radiation Lesions in Squamous Epithelia

et al. 2000

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“…

PLATES CXXI-CXXIV THE histopathology of the skin changes after exposure to /?-radiation has been described in several reports (Snider and Raper, 1951 ;Moritz and Henriques, 1952;Lushbaugh, Storer and Hale, 1953), but in only one instance (Lushbaugh et al) has a histochemical study of enzymes been included. More recently the effects of X-radiation (Bruni and Mazza, 1962;Kurban and Farah, 1969) and of deuteron radiation (Cabrini et al, 1970) on some epidermal enzymes have been studied by histochemical techniques. In biochemical studies of alterations in specific phosphatases (Tabachnick et al, 1967) and nucleases (Tabachnick, 1961 ;Tabachnick, Perlish and Freed, 1964) in ,%irradiated guinea-pig skin, we have found no evidence of an early increase in these hydrolases that could account for cell death.

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confidence: 99%