Microshoot culture of Persian violet: Plant regeneration and in vitro flowering

Sarai, Nisakorn; Bodhipadma, Kitti; Noichinda, Sompoch; Luangsriumporn, Parutuch; Leung, David W. M.

doi:10.1016/j.aoas.2017.05.006

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…However, effective micropropagation via microshoots and in vitro flower initiation has been achieved in the last six years. Recently, UV-C irradiation has been used to enhance root initiation and flower mutation in vitro [4,9,28,29].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Hypoxic Environment Enhances Volatile Compound Production in Persian Violet Flowers

Noichinda,

Bodhipadma

2023

Horticulturae

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Flowers of Persian violet (Exacum affine Balf. f. ex Regel) that are grown in nature typically produce a scent. However, whether Persian violet flowers developed inside sterile containers produce odors has yet to be studied. Therefore, this research aimed to study and compare the effects of ex vitro and in vitro environments on the volatile composition of Persian violet flowers. Persian violet flowers obtained from an in vitro culture and potted plants were analyzed for volatile constituents using gas chromatography–mass spectrometry (GC-MS). The main constituent of the volatile compounds in the Persian violet flowers grown in both conditions was alcohol, with 3-hexen-1-ol, which produces a grassy-green odor, being the dominant substance. In addition, the in vitro Persian violet flowers contained the highest amount of ethanol, which produces a wine aroma—followed by the terpene alcohol β-citronellol, which produces a rose scent. However, 3-carene (citrus odor), caryophyllene (floral odor), humulene (woody odor), and β-ionone (floral odor) were detected onlyin Persian violet flowers grown in natural conditions. Therefore, these results indicate that hypoxia possibly occurred during plantlet growth in the in vitro environment and caused some different volatile compound production from that in natural conditions.

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Section: Discussionmentioning

confidence: 99%

In Vitro Hypoxic Environment Enhances Volatile Compound Production in Persian Violet Flowers

Noichinda,

Bodhipadma

2023

Horticulturae

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“…In vitro Persian violet plantlets were obtained and micropropagated as described previously [14]. Microshoots (2-3 mm long) were excised from the in vitro Persian violet plantlets and were then used for alginate encapsulation in this study.…”

Section: Plant Materialsmentioning

confidence: 99%

“…f. ex Regel) with its eye-catching purple flowers is popular for decorative purposes in Thailand and is mainly propagated vegetatively because propagation by seeds reduces this plant's fertility [13]. Micropropagation via microshoots encapsulated with a single alginate coating may be an additional tool to assist mass vegetative propagation of Persian violet [4,14] and short-term storage for germplasm exchange. It is, therefore, of interest to investigate the effect of alginate coating on the short-term storability of encapsulated Persian violet microshoots at 4 • C and 25 • C for up to 60 days.…”

Section: Introductionmentioning

confidence: 99%

“…We also investigated this possibility with encapsulated Persian violet microshoots. Since, in our previous study [14], it was found that Persian violet microshoots of the size of 2-3 mm were preferable for micropropagation, we compared the storability of a synthetic seed (a microshoot of 2-3 mm encapsulated with a single layer of alginate) and a larger encapsulated structure (termed herewith as synthetic fruit) with five microshoots each of 2-3 mm. The emergernce or 'germination' of a single microshoot from the artificial alginate coating is analogous to the embryo which emerges from a true seed and hence the encapsulated microshoot is termed herewith as a synthetic seed.…”

Section: Introductionmentioning

confidence: 99%

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Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Phanomchai

Bodhipadma

Noichinda

et al. 2022

Plants

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Microshoots have been widely used for micropropagation. It may be necessary to store microshoots for a short period of time, for example in germplasm exchange needing transport to other research groups. Here, we investigated the short-term storability of alginate-encapsulated Persian violet (Exacum affine Balf. f. ex Regel) microshoots at 4 °C and 25 °C. After storage, the encapsulated microshoots were sown on basal Murashige and Skoog medium for germination and viability determination using tetrazolium chloride staining. The results showed that one or five microshoots encapsulated with a single alginate layer could be stored at 4 °C for up to 30 days, while the percentages of germination and viability of the microshoots encapsulated with two layers of alginate were greatly reduced upon storage. This is the first report on the storability of alginate-encapsulated multiple microshoots, which could be a more efficient way to encapsulate microshoots used for short-term cold storage.

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“…Persian violet, an ornamental plant having small, purple flowers with yellow pollen in its center, is mainly grown as a houseplant [15]. For micropropagation, microshoot explants of the Persian violet (1 mm long) have previously been used for multiple shoot induction [16,17]. In the present research, 5 mm long microshoots were employed as explants to initiate the development of multiple shoots on MS basal medium as an alternative to adding BAP.…”

Section: Plantlet Establishmentmentioning

confidence: 99%

Differing In Vitro Rooting and Flowering Responses of the Persian Violet to Low and High UV-C Irradiation

Phanomchai

Noichinda

Kachonpadungkitti

et al. 2021

Plants

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Persian violet flowers are considered esthetically attractive, leading to the high economic value of this plant. Plant breeding is fundamental to crop improvement, and the induction of mutation by tissue culture technology in combination with irradiation has been beneficially applied to generate plants with novel desirable characteristics. In this research, single or double rounds of UV-C irradiations were carried out on plant tissue cultures to initiate the in vitro rooting and mutation of Persian violets. It was found that single low-intensity UV-C exposure, when applied to Persian violet microshoots for 4 h, could induce the maximum number of roots and the highest root length without the use of a plant growth regulator. Overall, the single and double UV-C irradiation of Persian violet microshoots led to 44 different types of Persian violet flower mutations. Under single high-intensity UV-C irradiation for 6 h, up to nine petals were initiated, whereas single low-intensity UV-C irradiation did not influence the morphological variation of Persian violet flowers. Thus, Persian violet microshoots respond differently in terms of in vitro rooting and flowering depending on the UV-C intensity and exposure duration. These outcomes may be applied to micropropagation and in vitro plant breeding.

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Microshoot culture of Persian violet: Plant regeneration and in vitro flowering

Cited by 5 publications

References 17 publications

In Vitro Hypoxic Environment Enhances Volatile Compound Production in Persian Violet Flowers

In Vitro Hypoxic Environment Enhances Volatile Compound Production in Persian Violet Flowers

Short-Term Storability of Alginate-Encapsulated Persian Violet Microshoots for Germplasm Exchange

Differing In Vitro Rooting and Flowering Responses of the Persian Violet to Low and High UV-C Irradiation

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