Microsensors for in vivo Measurement of Glutamate in Brain Tissue

Qin, Si; Zeyden, Miranda van der; Oldenziel, Weite Hendrik; Cremers, Thomas; Westerink, Ben H.C.

doi:10.3390/s8116860

Cited by 50 publications

(35 citation statements)

References 66 publications

(85 reference statements)

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“…Using implantable sensors allows for stable, long-term recording of a number of common analytes in the extracellular fluid (ECF) of the brain including oxygen (Lowry et al, 1996Lowry and Fillenz, 2001;Bolger and Lowry, 2005), glucose (Hu and Wilson, 1997;Fillenz and Lowry, 1998a;Lowry et al, 1998a,b,c;Lowry and Fillenz, 2001;Dixon et al, 2002), nitric oxide (Brown et al, 2009) and glutamate (Kulagina et al, 1999;McMahon et al, 2006aMcMahon et al, , 2006bMcMahon et al, , 2007Qin et al, 2008;Tian et al, 2009). Unlike other methods implanted into the brain tissue (Clark et al, 1958;Krolicki and Leniger-Follert, 1980;Doppenberg et al, 1998;Gupta et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

Kealy

Bennett

Lowry

2013

Journal of Neuroscience Methods

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h i g h l i g h t sWe show that average concentrations of hippocampal oxygen and glucose are 100.26 ± 5.76 M and 0.60 ± 0.06 mM respectively. We show that there are uncoupled changes in oxygen and glucose during neuronal activation. Anaesthesia and carbonic anhydrase inhibition both significantly increase hippocampal oxygen. Anaesthesia, dimethyl sulfoxide administration and carbonic anhydrase inhibition significantly increase hippocampal glucose. We show that changes in hippocampal metabolism can be detected in real time using constant potential amperometry. a r t i c l e i n f o t r a c tAmperometric sensors for oxygen and glucose allow for real time recording from the brain in freelymoving animals. These sensors have been used to detect activity-and drug-induced changes in metabolism in a number of brain regions but little attention has been given over to the hippocampus despite its importance in cognition and disease. Sensors for oxygen and glucose were co-implanted into the hippocampus and allowed to record for several days. Baseline recordings show that basal concentrations of hippocampal oxygen and glucose are 100.26 ± 5.76 M and 0.60 ± 0.06 mM respectively. Furthermore, stress-induced changes in neural activity have been shown to significantly alter concentrations of both analytes in the hippocampus. Administration of O 2 gas to the animals' snouts results in significant increases in hippocampal oxygen and glucose and administration of N 2 gas results in a significant decrease in hippocampal oxygen. Chloral hydrate-induced anaesthesia causes a significant increase in hippocampal oxygen whereas treatment with the carbonic anhydrase inhibitor acetazolamide significantly increases hippocampal oxygen and glucose. These findings provide real time electrochemical data for the hippocampus which has been previously impossible with traditional methods such as microdialysis or ex vivo analysis. As such, these sensors provide a window into hippocampal function which can be used in conjunction with behavioural and pharmacological interventions to further elucidate the functions and mechanisms of action of the hippocampus in normal and disease states.

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Section: Introductionmentioning

confidence: 99%

Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

Kealy

Bennett

Lowry

2013

Journal of Neuroscience Methods

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“…Glutamate is not electrochemically active and therefore an enzyme is utilized for the electrochemical detection of glutamate [2,3,4]. Recently, an enzyme-free method for the detection of glutamate was developed [5], but, unfortunately, this approach requires highly alkaline conditions for proper function.…”

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confidence: 99%

Glutamate detection by amino functionalized tetrahedral amorphous carbon surfaces

Kaivosoja

Tujunen

Jokinen

et al. 2015

Talanta

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In this paper, a novel amperometric glutamate biosensor with glutamate oxidase (GlOx) immobilized directly on NH2 functionalized, platinum doped tetrahedral amorphous carbon (ta-C) film, has been successfully developed. First, we demonstrate that direct GlOx immobilization is more effective on amino-groups than on carboxyl-or hydroxyl-groups. Second, we show that anodizing and plasma treatments increase the amount of nitrogen and the proportion of protonated 2 amino groups relative to amino groups on the aminosilane coating, which subsequently results in an increased amount of active GlOx on the surface. This effect, however, is found to be unstable due to unstable electrostatic interactions between GlOx and NH3 + . We demonstrate the detection of glutamate in the concentration range of 10 µM−1mM using the NH2 functionalized Pt doped ta-C surface. The biosensor showed high sensitivity (2.9 nA μM -1 cm -2 ), low detection limit (10 μM) and good storage stability. The electrode response to glutamate was linear in the concentrations ranging from10 µM to 500 µM. In conclusion, the study shows that GlOx immobilization is most effective on aminosilane treated ta-C surface without any pre-treatments and the fabricated sensor structure is able to detect glutamate in the micromolar range.

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“…However, there are other interesting molecules to detect for clinical interest. Over the last two decades there have been proposed biosensors for lactate [31], cholesterol [43], glutamate [38], creatinine [21], etc. Biomarkers are another large family of biomolecules arising interest, since they are able to point out if a biological process, a disease, or a response to a therapeutic intervention is in progress.…”

Section: Targetsmentioning

confidence: 99%

Integrated biosensors for personalized medicine

Micheli

Boero

Baj-Rossi

et al. 2012

Proceedings of the 49th Annual Design Automation Conference

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Biosensors are heterogenous devices, incorporating biological structures combined with electronics, optical or other readout systems. They have been developed for detecting different biomolecules and/or pathogens and represent a key technology for advanced and pointof-care diagnostics as well as patient monitoring. In this paper we present a systematic classification of biosensors described in literature, particularly focusing on nanotechnology-based sensing. Then, we present our approach to develop electrochemical biosensors for measuring metabolites and anticancer drugs, based on a platform for multiple target detection. This platform is modular and achieves a clear separation between the chemical and the electrical components, thus easing design and manufacturing. It shows superior performance thanks to the excellent properties of electron transfer and selectivity showed by enzymes immobilized on carbon nanotubes.

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Microsensors for in vivo Measurement of Glutamate in Brain Tissue

Cited by 50 publications

References 66 publications

Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

Simultaneous recording of hippocampal oxygen and glucose in real time using constant potential amperometry in the freely-moving rat

Glutamate detection by amino functionalized tetrahedral amorphous carbon surfaces

Integrated biosensors for personalized medicine

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