Microscopic Methods for Characterization of Selected Surface Properties of Biodegradable, Nanofibrous Tissue Engineering Scaffolds

Chlanda, Adrian; Kijeńska, Ewa; Święszkowski, Wojciech

doi:10.4028/www.scientific.net/msf.890.213

Cited by 13 publications

(3 citation statements)

References 8 publications

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“…The surface of the scaffold was investigated using an atomic force microscopy technique (AFM). Considering the microscope’s working principles based on the mechanical contact between the scanning probe and tested surface, it is possible to extract additional information about the material. , An MFP 3D Bio (Oxford Instruments/Asylum Research) microscope was utilized for imaging the topography of the scaffolds’ surface at the micro- and nanoscale and for determining the surface roughness. The microscope was equipped with standard rectangular probes (RC800PSA; Olympus).…”

Section: Methodsmentioning

confidence: 99%

Surface Modification of 3D Printed Polycaprolactone Constructs via a Solvent Treatment: Impact on Physical and Osteogenic Properties

Kosik-Kozioł

Graham

Jaroszewicz

et al. 2018

ACS Biomater. Sci. Eng.

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One promising strategy to reconstruct bone defects relies on 3D printed porous structures. In spite of several studies having been carried out to fabricate controlled, interconnected porous constructs, the control over surface features at, or below, the microscopic scale remains elusive for 3D polymeric scaffolds. In this study, we developed and refined a methodology which can be applied to homogeneously and reproducibly modify the surface of polymeric 3D printed scaffolds. We have demonstrated that the combination of a polymer solvent and the utilization of ultrasound was essential for achieving appropriate surface modification without damaging the structural integrity of the construct. The modification created on the scaffold profoundly affected the macroscopic and microscopic properties of the scaffold with an increased roughness, greater surface area, and reduced hydrophobicity. Furthermore, to assess the performance of such materials in bone tissue engineering, human mesenchymal stem cells (hMSC) were cultured in vitro on the scaffolds for up to 7 days. Our results demonstrate a stronger commitment toward early osteogenic differentiation of hMSC. Finally, we demonstrated that the increased in the specific surface area of the scaffold did not necessarily correlate with improved adsorption of protein and that other factors, such as surface chemistry and hydrophilicity, may also play a major role.

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Section: Methodsmentioning

confidence: 99%

Surface Modification of 3D Printed Polycaprolactone Constructs via a Solvent Treatment: Impact on Physical and Osteogenic Properties

Kosik-Kozioł

Graham

Jaroszewicz

et al. 2018

ACS Biomater. Sci. Eng.

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“…Atomic force microscope (AFM) was used for topography imaging, surface evaluation at the nanoscale and evaluation of the particle shapes [35]. The sample preparation protocol was as follows: A water suspension of nanopowder was prepared at a concentration of 0.1 mg/mL.…”

Section: Methodsmentioning

confidence: 99%

Characterization and influence of hydroxyapatite nanopowders on living cells

Oberbek

Bolek

Chlanda

et al. 2018

Beilstein J. Nanotechnol.

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Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and respiratory systems.

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“…Atomic force microscope (Asylum Research AFM MFP3D Bio) was the primary equipment used for surface imaging . An Olympus AC 200TS cantilever was used in the tapping mode.…”

Section: Methodsmentioning

confidence: 99%

Tuning the Wettability of a Thin Polymer Film by Gradually Changing the Geometry of Nanoscale Pore Edges

et al. 2019

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Controlling wetting of solids by liquids attracts attention because of its scientific and technological importance. In this paper, the wettability of a highly uniform porous poly(methyl methacrylate) film on a silicon substrate containing a high density of randomly distributed self-similar pores was gradually tuned by changing the shape of nanometric crownlike structures around the pores. Fine-tuning the topography of these thin films was performed by isothermal annealing. The equilibrium contact angle of a water droplet placed on the surface of the films could be varied from 72 to 102°. The contact angle changes were assumed to be a consequence of changes in surface topography in the nanoscale. A simple method of a quantitative description of the change of the topography of these films was developed. Critical dimensions of these films were determined in horizontal and vertical directions relative to the surface plane. The slope coefficient (SC) describing how sharp the structures are, is defined as the ratio between the critical dimensions: the root-mean-square roughness σ and the autocorrelation length ξ. For SC > 0.08, the contact angle increased proportionally to the value of SC, whereas for SC < 0.08, the contact angle proportionally decreased. At the highest SC values, the contact angles were 6−10% higher than those predicted for flat porous surfaces using the Cassie− Baxter equation. We suggest that this discrepancy is due to the capillary tension caused by the submicron-scale undulation of the triple line, which was found to be proportional to the height of the crownlike pore edges and the value of SC. The same effect is responsible for the linear dependence of the contact angle on the SC value.

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Microscopic Methods for Characterization of Selected Surface Properties of Biodegradable, Nanofibrous Tissue Engineering Scaffolds

Cited by 13 publications

References 8 publications

Surface Modification of 3D Printed Polycaprolactone Constructs via a Solvent Treatment: Impact on Physical and Osteogenic Properties

Surface Modification of 3D Printed Polycaprolactone Constructs via a Solvent Treatment: Impact on Physical and Osteogenic Properties

Characterization and influence of hydroxyapatite nanopowders on living cells

Tuning the Wettability of a Thin Polymer Film by Gradually Changing the Geometry of Nanoscale Pore Edges

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