Kajal is an ancient eye cosmetic, traditionally used in mascara. It is widely used in the Middle East, the Mediterranean, South Asia, and the Horn of Africa as eyeliner to contour and/or darken the eyelids. Some did this to "strengthen the child's eyes", and others believed it could prevent the child from being cursed by the evil eye. With consideration to all these facts, herbal Kajal was formulated and evaluated. Herbal Microwave assisted extracted Kajal (MAEK) and Traditional method extracted Kajal (TMEK) were formulated by using Anethumgraveolans, Yasthimadhu, Ricinuscommunis, Ocimum sanctum, Castor oil and almond oil but the methods of extraction of active constituents was different and evaluated on various parameters. In MAEK all the herbs were extracted by using Microwave assisted extraction method. Green chemistry and microwave assisted extraction was used to obtain high yield, short extraction time, reducing waste and promoting efficiency in extraction. In TMEK all the herbs were extracted by using traditional methods of extraction. The pH, viscosity values proved the significant evaluation. The base satisfied the evaluated parameter values and physical evaluation was suggestive of a cosmetically appealing product.Composition of nutrient agar I.P and cylinder plate method was employed; MAEK showed higher level of zone of inhibition in microbial contamination as compared with TMEK. MAEK was able to inhibit protein denaturation in a concentration-dependent manner as compared with TMEK. Inhibition % of protein denaturation of the formulations was within the range from 53.0% to 76.0% at the concentration range of 25–100 ug/ml. MAEK exhibited a significantly higher level of inhibition compared to TMEK showed the lowest inhibition levels. Herbal Kajal prepared by using Microwave assisted extraction method showed better evaluation parameters as compared with TMEK.