Microscale compressive behavior of hydrated lamellar bone at high strain rates

Peruzzi, Cinzia; Ramachandramoorthy, Rajaprakash; Groetsch, Alexander; Casari, Daniele; Grönquist, Philippe; Rüggeberg, Markus; Michler, Johann; Schwiedrzik, Jakob

doi:10.1016/j.actbio.2021.07.005

Cited by 15 publications

(5 citation statements)

References 79 publications

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“…The strain-rate sensitivity exponent for the fresh, formalin-fixed and dehydration samples were 0.043, 0.038, and 0.013, respectively. The strain rate sensitivity values calculated in this study were within the range of previous studies on cortical bone (Carter and Hayes, 1976;Peruzzi et al, 2021). Although the effects of formalin preservation and dehydration preservation on the ultimate stress, ultimate strain and elastic modulus are debatable, these preservation methods significantly reduced the strain rate dependence of cortical bone compared with fresh bone.…”

Section: Figure 11supporting

confidence: 76%

Effects of different preservation on the mechanical properties of cortical bone under quasi-static and dynamic compression

Qiu

Liao²,

Xiang³

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Mechanical properties of biological tissue are important for numerical simulations. Preservative treatments are necessary for disinfection and long-term storage when conducting biomechanical experimentation on materials. However, few studies have been focused on the effect of preservation on the mechanical properties of bone in a wide strain rate. The purpose of this study was to evaluate the influence of formalin and dehydration on the intrinsic mechanical properties of cortical bone from quasi-static to dynamic compression.Methods: Cube specimens were prepared from pig femur and divided into three groups (fresh, formalin, and dehydration). All samples underwent static and dynamic compression at a strain rate from 10−3 s−1 to 103 s−1. The ultimate stress, ultimate strain, elastic modulus, and strain-rate sensitivity exponent were calculated. A one-way ANOVA test was performed to determine if the preservation method showed significant differences in mechanical properties under at different strain rates. The morphology of the macroscopic and microscopic structure of bones was observed.Results: The results show that ultimate stress and ultimate strain increased as the strain rate increased, while the elastic modulus decreased. Formalin fixation and dehydration did not affect elastic modulus significantly whereas significantly increased the ultimate strain and ultimate stress. The strain-rate sensitivity exponent was the highest in the fresh group, followed by the formalin group and dehydration group. Different fracture mechanisms were observed on the fractured surface, with fresh and preserved bone tending to fracture along the oblique direction, and dried bone tending to fracture along the axial direction.Discussion: In conclusion, preservation with both formalin and dehydration showed an influence on mechanical properties. The influence of the preservation method on material properties should be fully considered in developing a numerical simulation model, especially for high strain rate simulation.

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Section: Figure 11supporting

confidence: 76%

Effects of different preservation on the mechanical properties of cortical bone under quasi-static and dynamic compression

Qiu

Liao²,

Xiang³

et al. 2023

Front. Bioeng. Biotechnol.

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“…The elastic modulus, yield stress, and hardening/softening behavior of bone ECM micropillars significantly depend on the orientation of the mineralized collagen fibers (MCFs). [ 20 , 25 , 26 ] Additionally, the mechanical properties are generally higher at the lamellar level than at the macroscopic level. This size effect can be explained by stress concentrations produced by resorption spaces, lacunar and vascular porosity, defects, microcracks, and interfaces present at the macroscopic level that turns bone into a quasi‐brittle material.…”

Section: Introductionmentioning

confidence: 99%

Tensile Mechanical Properties of Dry Cortical Bone Extracellular Matrix: A Comparison Among Two Osteogenesis Imperfecta and One Healthy Control Iliac Crest Biopsies

Indermaur,

Casari,

Kochetkova

et al. 2023

JBMR Plus

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Osteogenesis imperfecta (OI) is a genetic, collagen‐related bone disease that increases the incidence of bone fractures. Still, the origin of this brittle mechanical behavior remains unclear. The extracellular matrix (ECM) of OI bone exhibits a higher degree of bone mineralization (DBM), whereas compressive mechanical properties at the ECM level do not appear to be inferior to healthy bone. However, it is unknown if collagen defects alter ECM tensile properties. This study aims to quantify the tensile properties of healthy and OI bone ECM. In three transiliac biopsies (healthy n = 1, OI type I n = 1, OI type III n = 1), 23 microtensile specimens (gauge dimensions 10 × 5 × 2 μm3) were manufactured and loaded quasi‐statically under tension in vacuum condition. The resulting loading modulus and ultimate strength were extracted. Interestingly, tensile properties in OI bone ECM were not inferior compared to controls. All specimens revealed a brittle failure behavior. Fracture surfaces were graded according to their mineralized collagen fibers (MCF) orientation into axial, mixed, and transversal fracture surface types (FST). Furthermore, tissue mineral density (TMD) of the biopsy cortices was extracted from micro–computed tomogra[hy (μCT) images. Both FST and TMD are significant factors to predict loading modulus and ultimate strength with an adjusted R2 of 0.556 (p = 2.65e−05) and 0.46 (p = 2.2e−04), respectively. The influence of MCF orientation and DBM on the mechanical properties of the neighboring ECM was further verified with quantitative polarized Raman spectroscopy (qPRS) and site‐matched nanoindentation. MCF orientation and DBM were extracted from the qPRS spectrum, and a second mechanical model was developed to predict the indentation modulus with MCF orientation and DBM (R2 = 67.4%, p = 7.73e−07). The tensile mechanical properties of the cortical bone ECM of two OI iliac crest biopsies are not lower than the one from a healthy and are primarily dependent on MCF orientation and DBM. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

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“…It measures vapor sorption under controlled relative humidity and temperature. By determination of the sorption isotherm for each humidity value, the corresponding water content at a constant temperature can be estimated ( Grönquist et al, 2019 ; Peruzzi et al, 2021 ). In contrast to the technique used in this study, achieving a reproducible wet state in a short amount of time is an advantage of DVS.…”

Section: Discussionmentioning

confidence: 99%

Bone collagen tensile properties of the aging human proximal femur

Bracher,

Voumard,

Simon

et al. 2024

Bone Reports

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Microscale compressive behavior of hydrated lamellar bone at high strain rates

Cited by 15 publications

References 79 publications

Effects of different preservation on the mechanical properties of cortical bone under quasi-static and dynamic compression

Effects of different preservation on the mechanical properties of cortical bone under quasi-static and dynamic compression

Tensile Mechanical Properties of Dry Cortical Bone Extracellular Matrix: A Comparison Among Two Osteogenesis Imperfecta and One Healthy Control Iliac Crest Biopsies

Bone collagen tensile properties of the aging human proximal femur

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