Microsatellites as markers for Australian wheat improvement

Harker, Natalie; Rampling, Lynette; Shariflou, M. R.; Hayden, Matthew; Holton, Timothy A; Morell, Michael K; Sharp, P. J.; Henry, Robert J; Edwards, Keith J.

doi:10.1071/ar01025

Cited by 29 publications

(12 citation statements)

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“…For example, Byran et al (1997), Röder et al (1998) and Pestova et al (2000) reported that on average 39% (32, 41 and 44%, respectively) of all primer pairs developed from the sequences of wheat microsatellite clones were functional. Moreover, the average PIC of the compound SSRs revealing polymorphism among the Australian cultivars was similar to that reported by Harker et al (2001) for published SSRs screened on the same set of cultivars (0.56 and 0.57, respectively). These results demonstrate that anchored PCR is suitable for the development of polymorphic compound SSR markers in bread wheat.…”

Section: Discussionsupporting

confidence: 79%

A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs

et al. 2003

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A study was undertaken to determine the utility in bread wheat of anchored PCR for the development of single locus SSR markers targeted at compound repeat motifs. In anchored PCR, microsatellite amplification is achieved using a single primer complementary to the flanking sequence, and one which anchors to the repeat junction of the compound SSR. The recovery rate of useable markers was found to be similar (43%) to that reported for conventionally generated SSRs. Thus, anchored PCR can be used to reduce the costs of marker development, since it requires that only half the number of primers be synthesised. Where fluorescence-based platforms are used, marker deployment costs are lower, since only the anchoring primers need to be labelled. In addition, anchored PCR improves the recovery of useful markers, as it allows assays to be generated from microsatellite clones with repeat sequences located close to their ends, a situation where conventional PCR amplification fails as two flanking primers cannot be designed. Strategies to permit the large-scale development of compound SSR markers amplified by anchored PCR are discussed.

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Section: Discussionsupporting

confidence: 79%

A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs

et al. 2003

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“…Allele sizes for Panel 2 were generated by the Southern Cross University (SCU), Australia, using fluorescently labelled SSR markers with the products analysed using capillary electrophoresis as described by Harker et al (2001).…”

Section: Methods Of Pcr Analysis and Scoringmentioning

confidence: 99%

Potential of SSR markers for plant breeding and variety identification in Australian barley germplasm

Karakousis¹,

Barr²,

Chalmers³

et al. 2003

Aust. J. Agric. Res.

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SSR markers closely linked to 18 loci that control 16 important barley traits were assessed for their applicability in Australian barley breeding programs. A panel of 40 genotypes routinely used by the South Australian Barley Improvement Program (SABIP) was used to examine the usefulness of these SSR markers for marker assisted selection (MAS). The success of monitoring a trait locus from donor to recipient lines ranged from 10 to 98%, depending on the marker. SSRs with a high polymorphic information content (PIC) value were found to be the most useful for application in MAS. The assessment also indicated that SSRs derived from genomic sequences were more successful for MAS than those designed from expressed sequence tags. A total of 130 SSR markers were screened among 2 panels of Australian barley genotypes to determine which markers would be the most useful for discriminating Australian germplasm. PIC values generated by this screening were also compared with those generated using a panel of European barley genotypes. Using ordinary correlations (parametric), rank correlations (non-parametric), and partial correlations (multi-variate), a strong association was found between the 2 Australian panels, but no or weak correlation was observed between the 2 Australian panels and the European dataset. It can therefore be concluded that PIC values generated by SSR markers screened with European genotypes cannot be used to predict the usefulness of an SSR marker for discriminating Australian genotypes. From PIC values generated in this study, 36 SSR markers have been selected for the discrimination of Australian genotypes. These markers all show high and/or consistent PIC values among Australian and European barley genotypes.A R 0 2 1 7 8 S S R m a r k e r s f o r p l a n t b r e e d i n g i n b a r l e y A . K a r a k o u s i s e t a l .

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“…More importantly, comparative mapping may become more widely practiced (Laurie & Devos, 2002). The availability of high-density consensus maps greatly facilitates the construction of new maps and mapping specific chromosomal regions Harker et al, 2001;Karakousis et al, 2003;Lefebvre et al, 2002;Lombard & Delourme, 2001). Comparative mapping could be used to make considerable progress in QTL mapping between related species.…”

Section: Future Trendsmentioning

confidence: 99%

An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts

Collard

Jahufer

Brouwer³

et al. 2005

Euphytica

1,386

914

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Recognizing the enormous potential of DNA markers in plant breeding, many agricultural research centers and plant breeding institutes have adopted the capacity for marker development and marker-assisted selection (MAS). However, due to rapid developments in marker technology, statistical methodology for identifying quantitative trait loci (QTLs) and the jargon used by molecular biologists, the utility of DNA markers in plant breeding may not be clearly understood by non-molecular biologists. This review provides an introduction to DNA markers and the concept of polymorphism, linkage analysis and map construction, the principles of QTL analysis and how markers may be applied in breeding programs using MAS. This review has been specifically written for readers who have only a basic knowledge of molecular biology and/or plant genetics. Its format is therefore ideal for conventional plant breeders, physiologists, pathologists, other plant scientists and students.

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Microsatellites as markers for Australian wheat improvement

Cited by 29 publications

References 0 publications

A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs

A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs

Potential of SSR markers for plant breeding and variety identification in Australian barley germplasm

An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts

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