Microsatellite Polymorphism in Japanese Mongrel Dogs

Oishi, Noboru; Maeda, Morihiro; Makimura, Koichi; Sawaguchi, Toshiko; Hayashiya, Makio; Kubo, Takuya; Kano, Rui; Hasegawa, Atsuhiko; Kasahara, Michihiro

doi:10.1292/jvms.67.1055

Cited by 3 publications

(5 citation statements)

References 23 publications

(24 reference statements)

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“…The Polymorphism Information Content (PIC), PIC values for the 26 micro satellite value ranged between 0.6844 (AHTk211) and 0.8757 (FH2326) with a mean value of 0.8117. In the present study, the percentage of polymorphic loci obtained is 100 as all the 26 microsatellite loci studied were , Oishi et al, 2005, Puja et al, 2005, however in studies conducted by Lupke and Distl (2004) and Ciampolini et al (2011) in other breeds revealed that the population was found to be in HW E at certain loci AHTk211, FH2054, INRA21, INU055 and REN169018 loci and on the other hand showed significant deviation from HWE in six microsatellites deviation (AHTh260, CXX279, RENI05L03, REN54Pll, REN162C04 and AHTH130). Assessment of HWE in four native breeds of Tamil Nadu; Rajapalayam, Chippiparai, Kanni and Kombai by Hisham (2014) revealed that among 29 microsatellite loci studied loci INRA21 in both Rajapalayam and Chippiparai, FH2054 in Kanni and AHTh260 in Kombai were in Hardy-Weinberg disequilibrium.…”

Section: Resultscontrasting

confidence: 54%

“…Microsatellite alleles, their number, allele sizes and their frequencies at different loci are furnished in Table 1 and 2. A total of 221 alleles were identified in this study, whereas Hisham (2014) observed a total of 297 alleles in four native breeds of Tamil Nadu, viz., Rajapalayam, Chippiparai, Kanni and Kombai using 29 microsatellite markers and lesser allele number were reported in mongrels of Japan and Kinatamani dogs of Bali, Indonesia, South Korean dogs (Oishi et al, 2005, Puja et al, 2005, Kang et al, 2009. The number of alleles ranged from 5 (AHTk211) to 12 (INU055, PEZ12) with a mean of 8.5±1.9442 alleles.…”

Section: Resultsmentioning

confidence: 96%

“…The observed heterozygosity ranged between 0.26 (PEZ11) and 1 (AHTh260, AHTk211, INU005 and REN169D01) and the expected heterozygosity ranged from 0.7282 (AHTk211) to 0.8956 (PEZ12). The results were in agreement with that studied in Golden Retriever, Labrador Retriever, Rottweiler breeds, Japanese Mongrel, Kintamani dogs of Bali, Rajapalayam, Chippiparai and Konbai breeds (Koskinen and Bredbacka., 2000, Altet et al, 2001, Oishi et al, 2005., Puja et al, 2005., Hisham, 2014, Anna Radko et al, 2015.…”

Section: Resultsmentioning

confidence: 99%

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Microsatellite DNA Polymorphism Studies in Mudhol Hound Dog Native of India

Sowmyashree¹,

Rout²,

Kumar³

et al. 2021

IJAR

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Background: Mudhol Hound the popular dog breed of Karnataka State, India has a long historical association with wars, hunting, social bondage, guarding and recently with the Indian army as a part of army’s sniffer dog squad. Despite its unique qualities, the number of animals is getting reduced gradually. Hence the present study was aimed at molecular characterization of the Mudhol Hound which will be useful in maintenance of diversity of the breed. Methods: In this study, 26 microsatellite markers were used for the characterization of Mudhol Hound dog. A total of 50 blood samples were screened. Microsatellite loci were analyzed by running the PCR products on 6.5 per cent polyacrylamide gels followed by silver staining. Genotyping was done based on the banding patterns. Result: A total of 221 alleles were observed from the 26 microsatellites. The number of alleles ranged from 5 (AHTk211) to 12 (INU055, PEZ12) with a mean of 8.5±1.9442 alleles. The average H0 and He were 0.7685±0.2122 and 0.8326±0.0397, respectively. The percentage of polymorphic loci obtained was 100 as all the loci studied were found to be polymorphic having the PIC values more than 0.5. All the 26 loci showed significant deviation from Hardy - Weinberg equilibrium (P less than 0.001).

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Section: Resultscontrasting

confidence: 54%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

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Microsatellite DNA Polymorphism Studies in Mudhol Hound Dog Native of India

Sowmyashree¹,

Rout²,

Kumar³

et al. 2021

IJAR

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“…Their abundance in the genome, high levels of polymorphism, Mendelian fashion of inheritance, and high-throughput ability enables microsatellites markers to be a useful tool in the diagnosis of inherited diseases, parentage testing, forensic medicine, studies of genetic differentiation, and population studies for many species (Zajc et al, 1997;Altet et al, 2001). A number of canine microsatellite markers have been widely used in the field of kinship analysis for breeding purposes in Austria (using 15 markers), Italy (using 10 markers), Japan (using 10 markers), and United States (using 17 markers) (DeNise et al, 2004;Eichmann et al, 2004;Gentilini et al, 2004;Oishi et al, 2005). The standardization of number and type of markers for parentage test has been attempted under the auspices of the International Society for Animal Genetics due to the variety of present verification system (Halverson and Edwards, 2000).…”

mentioning

confidence: 99%

Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

Kang

Kim²,

Min³

et al. 2009

Genes Genet. Syst.

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To investigate the population structure of five dog breeds in South Korea and to validate polymorphic microsatellite markers for the parentage test, microsatellite loci analyses were conducted for two Korean native dog breeds, Poongsan and Jindo, and three imported dog breeds, German Shepherd, Beagle and Greyhound. Overall genetic diversity was high across all dog breeds (expected heterozygosity range: 0.71 to 0.85), although breeds differed in deviations from Hardy-Weinberg equilibrium (HWE). Significant reduction of heterozygosity in the Poongsan and Greyhound breeds was caused by non-random mating and population substructure within these breeds (the Wahlund effects). The close relationship and high degree of genetic diversity for two Korean native dog breeds were substantial. The mean polymorphism information content value was highest in Jindos (0.82) and Poongsans (0.81), followed by Beagles (0.74), Greyhounds (0.72), and German Shepherds (0.66). Accumulated exclusion power values, as an indication of marker validity for parentage tests, were varied but very high across breeds, 0.9999 for Jindos, Poongsans, and Beagles, 0.9997 for Greyhounds, and 0.9995 for German Shepherds. Taken together, the microsatellite loci investigated in this study can serve as suitable markers for the parentage test and as individual identification to establish a reliable pedigree verification system of dog breeds in South Korea. This study also stresses that the population subdivision within breeds can become an important cause of deviation from HWE in dog breeds.

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“…Canine genotyping has been particularly performed in non-forensic fields in order to characterize the genetic structure and the diversity within and among dog breeds and wolf populations (e. g. [31][32][33][34][35][36][37][38][39][40][41][42][43]). The majority of these studies were motivated by conservation concerns and tried to assess population structure, genetic diversity and inbreeding in endangered populations or small breeds (e. g. [30,[44][45][46][47][48][49][50][51][52][53][54][55][56][57][58]).…”

Section: Brief Historymentioning

confidence: 99%

State-of-the-Art and Future Prospects of Canine STR-Based Genotyping

Asch¹,

Pereira²

2012

TOFORSJ

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The dog is the most common domestic animal in human environments and in many situations a dog may be a victim, a perpetrator or a link between a suspect and a crime scene. Therefore, biological material derived from dogs may constitute evidence in forensic caseworks and it may be necessary or helpful to obtain genetic profiles that would aid individual identification. Currently, the assessment of the genetic diversity of breeds, population structure, inbreeding, and the development of methodologies for population assignment are important areas of research in dogs and related species such as the grey wolf. Therefore, canine short tandem repeat (STR)-based genotyping is used by a significant number of population geneticists; however, for reasons we present here, it is utilized by a relatively small number of forensic practitioners. An extensive bibliographic search revealed a highly fragmented canine genotyping community working under less than well defined standards. In this work, we discuss the present developments and limitations of STR-based canine genotyping. Furthermore, we recommend that a collaborative strategy for the implementation of standardization and harmonization is crucial to the development of forensic canine genotyping.

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Microsatellite Polymorphism in Japanese Mongrel Dogs

Cited by 3 publications

References 23 publications

Microsatellite DNA Polymorphism Studies in Mudhol Hound Dog Native of India

Microsatellite DNA Polymorphism Studies in Mudhol Hound Dog Native of India

Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

State-of-the-Art and Future Prospects of Canine STR-Based Genotyping

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