Microsatellite loci analysis for the genetic variability and the parentage test of five dog breeds in South Korea

Abstract

To investigate the population structure of five dog breeds in South Korea and to validate polymorphic microsatellite markers for the parentage test, microsatellite loci analyses were conducted for two Korean native dog breeds, Poongsan and Jindo, and three imported dog breeds, German Shepherd, Beagle and Greyhound. Overall genetic diversity was high across all dog breeds (expected heterozygosity range: 0.71 to 0.85), although breeds differed in deviations from Hardy-Weinberg equilibrium (HWE). Significant reduction of heterozygosity in the Poongsan and Greyhound breeds was caused by non-random mating and population substructure within these breeds (the Wahlund effects). The close relationship and high degree of genetic diversity for two Korean native dog breeds were substantial. The mean polymorphism information content value was highest in Jindos (0.82) and Poongsans (0.81), followed by Beagles (0.74), Greyhounds (0.72), and German Shepherds (0.66). Accumulated exclusion power values, as an indication of marker validity for parentage tests, were varied but very high across breeds, 0.9999 for Jindos, Poongsans, and Beagles, 0.9997 for Greyhounds, and 0.9995 for German Shepherds. Taken together, the microsatellite loci investigated in this study can serve as suitable markers for the parentage test and as individual identification to establish a reliable pedigree verification system of dog breeds in South Korea. This study also stresses that the population subdivision within breeds can become an important cause of deviation from HWE in dog breeds.

Statistical analysis and genetic diversity of three dog breeds using simple sequence repeats

“…They detected the highest genetic diversity in the Korean native dogs and the lowest in the Japanese native dog Shiba. Kang et al (2009) carried out parentage analysis in two Korean native dog breeds, Poongsan and Jindo, and three imported dog breeds, German shepherd, beagle and greyhound. Their results also indicate that the Korean dog breeds have greater diversity compared to the European dog breeds, although Poongsan, the Korean native dogs, had a significant reduction in heterozygosity.…”

Molecular genetic diversity of donkey (Equus asinus) in South Korea

“…In a single gene locus, the indicator that expresses the diversity of a group is heterozygosity. Heterozygosity is defined as “the probability that, when randomly two allelic genes are extracted from a group, the two may differ.” In an association analysis or linkage disequilibrium analysis, to be used as a marker, the higher the heterozygosity, the more desirable it becomes [10, 21, 25]. Even when the plural groups are mixed, of course, heterozygosity increases, but if there is no mix of groups, generally, heterozygosity is related to mutation.…”

“…Microsatellite has a high mutation rate over 1/10⁴~ 1/10⁶ per generation and has high viscosity according to the group. These characteristics show specific polymorphism at the object level as well as in the group, so it is used as a useful tool for genetic mapping as well as information about the hereditary diversity of animals, including human and plants [8, 10, 13, 14, 24].…”

Molekulargenetische Identifikation von Canidae in der forensischen Praxis

“…Die resultierenden Allelfrequenzen sind z. B. auch für die Abstammungs-und Mischlingsanalysen bei Hunden nutzbar [9][10][11][12]. …”