Microsatellite mining in the genus Colletotrichum

Highly transferable microsatellite markers for the genera Lasiodiplodia and Neofusicoccum

“…Cross-species transferable SSR markers have also been developed in other fungi, however these studies have also reported a low number of transferable markers (between four and eleven) (Wadud et al 2006;Cristancho & Escobar 2008;Benichou et al 2009;Leyva-Madrigal et al 2014). The largest collection of cross-species transferable SSR markers in a fungal genus, other than the present study is that of Bhat et al (2018), which developed thousands of SSR markers that were predicted to be capable of amplification across eight Colletotrichum species. The markers developed in the present study were not tested on species outside the genus for which they were developed.…”

“…Researchers adopting these markers will thus still need to confirm the amplification and polymorphism of these markers in the target population. Even though many studies have demonstrated SSR marker transferability among various fungal taxa (Wadud et al 2006;Cristancho & Escobar 2008;Benichou et al 2009;Leyva-Madrigal et al 2014;Bhat et al 2018), only a few have demonstrated the polymorphism of these markers in the non-target species (Cristancho & Escobar 2008;Bhat et al 2018). Despite the additional steps needed to select appropriate markers from collections presented here, the transferable markers still represent a useful starting point for marker development that is expected to significantly reduce time and cost.…”

“…cross-species transferability. Other studies have also used SSR-containing sequences, extracted from fungal genome sequences, to develop SSR markers (Jia et al 2015;Mercière et al 2015;Fortuna et al 2016;Wang & Chilvers 2016;Yu et al 2016;Vaghefi et al 2017;Bhat et al 2018;Mlonyeni et al 2018). These studies generally develop much larger numbers of SSR markers than studies using other approaches.…”

Fungal genomes enhance our understanding of the pathogens affecting trees cultivated in Southern Hemisphere plantations

“…This approach has been used to identify microsatellite markers for a wide range of fungi, including pathogens of trees important to plantation forestry in the tropics and Southern Hemisphere (Cai et al 2013;Simpson et al 2013;Jia et al 2015;Mercière et al 2015;Mlonyeni et al 2018;Varady et al 2019). In fact, using comparative genomics, makes it possible to design these markers such that they function across several species or even across genera (Leyva-Madrigal et al 2014;Bhat et al 2018). For example, Nagel et al (2020) recently developed microsatellite markers that can be amplified across species in the genera Lasiodiplodia and Neofusicoccum, numerous of which are important latent pathogens of plantation-grown trees (Slippers and Wingfield 2007;Slippers et al 2017).…”

Evaluation of Trichoderma asperellum Effect toward Anthracnose Pathogen Activity on Red Chili (Capsicum annum L.) As Ecofriendly Pesticide

“…In almost all over the world, chili always gets dangerous pathogenic disorders, including Colletotrichum spp., which causes losses up to 80% [1], [2]. Colletotrichum is a pathogenic fungus that is the main cause of damage and loss of chili yields in the tropics and subtropics regions [3], mostly Manuscript received August 22, 2021; revised November 10, 2021. This work was supported the Ministry of Research and Technology and Higher Education for the 2019 Applied Research Higher Education grant awarded under contract number 009/II.3.AU/14.00C/PRY/III/2019.…”