Highly transferable microsatellite markers for the genera Lasiodiplodia and Neofusicoccum

Nagel, Jan Hendrik; Cruywagen, Elsie M.; Machua, Joseph; Wingfield, Michael J.; Slippers, Bernard

doi:10.1016/j.funeco.2019.100903

Cited by 4 publications

(3 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, 60 primer pairs were developed and virtually amplified among genus Eucalyptus displayed interspecific polymorphism (30). Recently, 12 and 11 markers of Lasiodiplodia mahajangana and Neofusicoccum parvum tested for length variation and high transferability of the developed markers were observed (34).…”

Section: Transferability Of Common and Polymorphic Cpssrs In Genus Eucalyptusmentioning

confidence: 99%

See 1 more Smart Citation

Length variation of chloroplast simple sequence repeats in the genus Eucalyptus L'Hér.

Kumar

Shanker

2020

Plant Sci. Today

View full text Add to dashboard Cite

Eucalyptus L'Hér. is an economically important genus of plants with several environmental significances and great industrial advantages. To accelerate breeding and conservation studies, efforts on molecular breeding and molecular genetic analysis are underway in the genus Eucalyptus. Despite these efforts, no sufficient information is available about common, polymorphic and unique chloroplast simple sequence repeats (cpSSRs) in the genus Eucalyptus. . These repeats consist of 1-6 nucleotides and play important role in the development of molecular markers, genetic mapping and plant breeding. In the present study, a total of 920 cpSSRs were detected and length variation of cpSSRs analysed between each pair of species among 31 chloroplast genome sequences of the genus Eucalyptus. Additionally, cross species transferability of common and polymorphic cpSSRs were also observed. The common, unique and putative polymorphic cpSSRs analysed in this study can be used for species identification and genetic diversity studies of Eucalyptus.

show abstract

Section: Transferability Of Common and Polymorphic Cpssrs In Genus Eucalyptusmentioning

confidence: 99%

“…Recently, common and unique SSRs have also been identified in several species (13,19,20). Moreover, transferability of SSRs was observed between Croton floribundus and Croton urucurana (Euphorbiaceae;31) and genus Lasiodiplodia and Neofusicoccum (34).…”

Section: Introductionmentioning

confidence: 99%

Length variation of chloroplast simple sequence repeats in the genus Eucalyptus L'Hér.

Kumar

Shanker

2020

Plant Sci. Today

View full text Add to dashboard Cite

show abstract

“…The faulty genotyping profiles could have resulted due to non-target 11 primer annealing. The increased phylogenetic distance between the source (big cats: genus Panthera) and target (Eurasian lynx: genus Lynx) species greatly reduces transferability of markers54 .…”

mentioning

confidence: 99%

Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study

Hyun

Pandey

Kim

et al. 2020

Preprint

View full text Add to dashboard Cite

AbstractBig cats (Genus: Panthera) are among the most threatened mammal groups of the world, owing to illegal transnational trade. Conservation genetic studies and effective curbs on poaching are important for the conservation of these charismatic apex predators. A limited number of microsatellite markers exists for Panthera species and researchers often cross-amplify domestic cat microsatellites to study these species. We conducted data mining of seven Panthera genome sequences to discover microsatellites for conservation genetic studies of four threatened big cat species. A total of 32 polymorphic microsatellite loci were identified in silico and tested with 99 big cat individuals and 7 Eurasian lynx. The developed markers were polymorphic in most of the tested species. We propose a set of 15 novel microsatellite markers for use in conservation genetics and wildlife forensic investigations of big cat species. Cumulatively, these markers have a high discriminatory power of one in a million for unrelated individuals and one in a thousand for siblings. Similar PCR conditions of these markers increase the prospects of achieving efficient multiplex PCR assays. This study is a pioneering attempt to synthesise genome wide microsatellite markers for big cats.

show abstract

Next-generation sequencing provides important insights into the biology and evolution of the Botryosphaeriaceae

Nagel

Wingfield

Slippers

2021

Fungal Biology Reviews

View full text Add to dashboard Cite

Highly transferable microsatellite markers for the genera Lasiodiplodia and Neofusicoccum

Cited by 4 publications

References 57 publications

Length variation of chloroplast simple sequence repeats in the genus Eucalyptus L'Hér.

Length variation of chloroplast simple sequence repeats in the genus Eucalyptus L'Hér.

Whole genome survey of big cats (Genus: Panthera) identifies novel microsatellites of utility in conservation genetic study

Next-generation sequencing provides important insights into the biology and evolution of the Botryosphaeriaceae

Contact Info

Product

Resources

About