Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.)

Ek, Martin; Eklund, Martin; Post, R. von; Dayteg, Christophe; Henriksson, Tina; Weibull, P.; Ceplitis, Alf; Isaac, Peter G.; Tuvesson, Stine

doi:10.1111/j.1601-5223.2005.01906.x

Cited by 56 publications

(44 citation statements)

References 29 publications

(34 reference statements)

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“…Modern molecular markers have an array of applications, including marker-assisted selection (Ek et al, 2005), identification of regions affecting quantitative trait loci (Tar'an et al, 2005), and estimates of diversity (Baranger et al, 2004;Smýkal et al, 2008) in the study of pea. The current composite microsatellite map has 229 codominant SSR molecular markers (216 anonymous and 13 genic) spread over a 1430-cM (Haldane) map, yielding an average 6.2-cM distance between these reliable markers (Loridon et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

Kumari¹,

Basal²,

Singh³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The genetic diversity among 28 pea (Pisum sativum L.) genotypes was analyzed using 32 simple sequence repeat markers. A total of 44 polymorphic bands, with an average of 2.1 bands per primer, were obtained. The polymorphism information content ranged from 0.657 to 0.309 with an average of 0.493. The variation in genetic diversity among these cultivars ranged from 0.11 to 0.73. Cluster analysis based on Jaccard's similarity coefficient using the unweighted pair-group method with arithmetic mean (UPGMA) revealed 2 distinct clusters, I and II, comprising 6 and 22 genotypes, respectively. Cluster II was further differentiated into 2 subclusters, IIA and IIB, with 12 and 10 genotypes, respectively. Principal component (PC) analysis revealed results similar to those of UPGMA. The first, second, and third PCs contributed 21.6, 16.1, and 14.0% of the variation, respectively; cumulative variation of the first 3 PCs was 51.7%.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

Kumari¹,

Basal²,

Singh³

et al. 2013

Genet. Mol. Res.

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“…The detection of these genes by molecular markers might enable to address this problem. RAPD, SCAR and microsatellite markers linked to the gene er1 have already been described (Timmerman et al 1994, Tiwari et al 1998, Janila and Sharma 2004, Ek et al 2005. In addition, our group is currently carrying out the identification of molecular markers linked to Er3.…”

Section: Inheritance Of the Resistance To E Pisi From P Fulvummentioning

confidence: 99%

Identification of a New Gene for Resistance to Powdery Mildew in Pisum fulvum, a Wild Relative of Pea

et al. 2007

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“…Janila and Sharma (2004) and Ek et al (2005). However, bearing in mind the genotypes used for reference, e.g.…”

Section: Introductionmentioning

confidence: 99%

Two powdery mildew resistance mutations induced by ENU in Pisum sativum L. affect the locus er1

Pereira

Leitão

2009

Euphytica

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The alkylating compounds and strong mutagens methylnitrosourea (MNU) and ethylnitrosourea (ENU) were used to widen the genetic variability of pea (Pisum sativum L.) via experimental mutagenesis. Amongst multiple mutations of agronomical interest, for the first time two mutations conferring broad-range resistance to powdery mildew (Erysiphe pisi Syd.) were induced in pea. Mutagenic treatments were carried out on seedlings using a technique that ensures very high mutagenic efficiency. Two-hour exposure of cv. Solara seedlings to chemical mutagens resulted in almost non noticeable lethality and sterility in the M1 generation and very high mutation rates: *39% families with visible mutations in the M2 generation. The influence of the cell cycle phase on the process of mutagenesis was studied in cv. Frilene using a previously developed technique for synchronization of shoot apical meristem cells. The cell cycle phase at which cells were treated apparently did not influence the lethality and sterility in the M1 generation, while the visible mutation rate, assessed in the M2 generation, showed a clear cell cycle dependency. Seedlings treated at the G2 and M phases gave rise to progenies exhibiting the highest mutation rate, over 50% of M2 families with visible mutations. The powdery mildew resistant (PMR) mutant S(er1mut1) was induced by treatment of cv. Solara seedlings for 2 h with ENU, while the PMR mutant F(er1mut2) was induced by treatment of cv. Frilene seedlings with the same chemical mutagen for 1 h during the G2 phase of the cell cycle. The genetic analysis of the novel PMR mutant lines showed that both resistances are inherited as monogenic recessive traits. The performed genetic complementation analyses revealed that both mutations affect the same locus-er1, which determines most of the natural sources of PMR in pea. A project aiming at the isolation of the powdery mildew resistance mutated gene via map based cloning is currently under way.

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Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.)

Cited by 56 publications

References 29 publications

Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

Genetic diversity studies in pea (Pisum sativum L.) using simple sequence repeat markers

Identification of a New Gene for Resistance to Powdery Mildew in Pisum fulvum, a Wild Relative of Pea

Two powdery mildew resistance mutations induced by ENU in Pisum sativum L. affect the locus er1

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