Identification of a New Gene for Resistance to Powdery Mildew in Pisum fulvum, a Wild Relative of Pea

Fondevilla, Sara; Torres, A. M.; Moreno, M. T.; Rubiales, Diego

doi:10.1270/jsbbs.57.181

Cited by 87 publications

(70 citation statements)

References 21 publications

(22 reference statements)

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“…Among these, fungal diseases caused by Fusarium wilt, Ascochyta blight, and powdery mildew are the most economically important in the Mediterranean basin region (Rubiales et al, 2009). Until now, several resistance genes (R genes) conferring resistance against many diseases and pests have been identified in the pea, such as Fw and Fwf for resistance to Fusarium wilt (Coyne et al, 2000;Okubara et al, 2005); er1, er2, and er3 against powdery mildew (Tiwari et al, 1997;Fondevilla et al, 2007); and Ppi1, Ppi3, and Ppi4 that confer resistance against the pea bacterial blight disease caused by Pseudomonas syringae (Hunter et al, 2001). Despite the existence of well-known resistance genes in the pea, constant environmental changes and the adaptation of pathogens requires the investigation of germplasms, to identify new sources of resistance.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

Djebbi¹,

Bouktila²,

Makni³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Pea (Pisum sativum) is one of the most cultivated legumes in the world, and its yield and seed quality are affected by a variety of pathogens. In plants, NBS-LRR (nucleotide binding siteleucine-rich repeat) is the main class of disease resistance genes. Using degenerate primers deduced from conserved motifs in the NBS domain of known resistance genes, we identified 10 NBS sequences in three varieties of P. sativum. The deduced amino acid sequences of the identified resistance gene analogues (RGAs) exhibited the typical motifs of the NBS domain (P-loop, kinase-2, kinase-3a, and the hydrophobic domain, GLPL) present in the majority of plant proteins belonging to the NBS-LRR class. Phylogenetic analysis showed that seven RGAs belonged to the non-TIR-NBS-LRR subclass and three to the TIR-S. Djebbi et al. 6420©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6419-6428 (2015) NBS-LRR subclass. The results of this study provide insights into the structure of this class of resistance genes in the pea, and their evolutionary relationships with those of other plant species.

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Section: Introductionmentioning

confidence: 99%

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

Djebbi¹,

Bouktila²,

Makni³

et al. 2015

Genet. Mol. Res.

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“…Severe damage about 25-50% to the production could occur . Moreover, the number of pods per plant, seeds per pod, heights and the number of nodes could drastically decrease (Fondevilla et al, 2007). Therefore, chemical control of the disease is needed, but it increases cost of production.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the resistant er-3 gene has been found in P. fulvum which originated in eastern Mediterranean and Near East areas. The presence of resistant gene showed the dominant specific character and also depended on the climate (Fondevilla et al, 2007). Marker-assisted selection (MAS) may be used to shorten time needed to fix resistance to powdery mildew in segregating populations.…”

Section: Introductionmentioning

confidence: 99%

Study of Powdery Mildew Resistance in seven Lines of Snow Pea, Pisumsativum

Chompupoung¹,

Kumphai²,

Sittigul³

et al. 2012

JAS

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Snow pea is one of the important crops grown on highland of Thailand. However, it is very susceptible to powdery mildew. Thus, resistant cultivar increases the production of this crop at a reduced costs. Seven pea lines/cultivars, namely P117, P185, P309, No.3, No.4, No.5 and Fang No.7, were evaluated for phenotypic powdery mildew resistance at Pang Da Royal Agricultural Station, Samoeng district, Chiang Mai during the period of November, 2007 -March, 2008. They were divided into 3 groups: highly resistant group (R) i.e. P117, P185 and P309, moderately resistant group (M) i.e. No.3, No.4 and 5, and susceptible group (S) i.e. Fang No.7. In addition, DNA marker for powdery mildew resistance, er, was tested using PCR technique with 3 primers, ScOPD10, OPU17 and OPO02. It was found that only ScOPD10 could yield an 850-bp marker specific to resistant group. DNA sequencing of this 850 bp marker showed 618-624 bases. The sequence was compared www.ccsenet.org/jas Journal of Agricultural Science Vol. 4, No. 5; 2012 ISSN 1916-9752 E-ISSN 1916 210 with databases in NCBI GenBank. After BLAST searches, the 618-624 base sequences had 90-91% homology to the DNA sequence from clone JICPSV-598E15, complete sequence of P. sativum.

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“…More recently Fondevilla et al (2007Fondevilla et al ( , 2008 identified a resistance locus (Er3) in P. fulvum which they determined to be monogenic and dominantly inherited using interspecific crosses with P. sativum.…”

Section: Introductionmentioning

confidence: 99%

Two powdery mildew resistance mutations induced by ENU in Pisum sativum L. affect the locus er1

Pereira

Leitão

2009

Euphytica

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The alkylating compounds and strong mutagens methylnitrosourea (MNU) and ethylnitrosourea (ENU) were used to widen the genetic variability of pea (Pisum sativum L.) via experimental mutagenesis. Amongst multiple mutations of agronomical interest, for the first time two mutations conferring broad-range resistance to powdery mildew (Erysiphe pisi Syd.) were induced in pea. Mutagenic treatments were carried out on seedlings using a technique that ensures very high mutagenic efficiency. Two-hour exposure of cv. Solara seedlings to chemical mutagens resulted in almost non noticeable lethality and sterility in the M1 generation and very high mutation rates: *39% families with visible mutations in the M2 generation. The influence of the cell cycle phase on the process of mutagenesis was studied in cv. Frilene using a previously developed technique for synchronization of shoot apical meristem cells. The cell cycle phase at which cells were treated apparently did not influence the lethality and sterility in the M1 generation, while the visible mutation rate, assessed in the M2 generation, showed a clear cell cycle dependency. Seedlings treated at the G2 and M phases gave rise to progenies exhibiting the highest mutation rate, over 50% of M2 families with visible mutations. The powdery mildew resistant (PMR) mutant S(er1mut1) was induced by treatment of cv. Solara seedlings for 2 h with ENU, while the PMR mutant F(er1mut2) was induced by treatment of cv. Frilene seedlings with the same chemical mutagen for 1 h during the G2 phase of the cell cycle. The genetic analysis of the novel PMR mutant lines showed that both resistances are inherited as monogenic recessive traits. The performed genetic complementation analyses revealed that both mutations affect the same locus-er1, which determines most of the natural sources of PMR in pea. A project aiming at the isolation of the powdery mildew resistance mutated gene via map based cloning is currently under way.

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Identification of a New Gene for Resistance to Powdery Mildew in Pisum fulvum, a Wild Relative of Pea

Cited by 87 publications

References 21 publications

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

Study of Powdery Mildew Resistance in seven Lines of Snow Pea, Pisumsativum

Two powdery mildew resistance mutations induced by ENU in Pisum sativum L. affect the locus er1

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