Microsatellite markers for population studies of the rice blast fungus, Magnaporthe grisea

Abstract

We developed nine new microsatellite markers for rice blast (Magnaporthe grisea) population studies. These markers were used in addition to nine microsatellite markers previously developed by our group for mapping purpose. Altogether, the 18 markers were used in multiplex PCR (polymerase chain reaction) to characterize six populations from different geographical origins. The average number of alleles per locus across populations ranged from 1.2 to 7 and the total number of alleles detected from 2 to 19. Based on this large range of polymorphism, this set of markers is expected to be useful for different kind of population studies at different geographical scales.

Development of microsatellite markers and construction of genetic map in rice blast pathogen Magnaporthe grisea

“…The percentage of polymorphic SSRs observed here is very close to that (73%) reported by Kaye et al (2003). The number of alleles detected per locus ranges 2-9 with an average of 3.3, which is almost the same as the estimate (3.4) reported by Adreit et al (2007). The PIC value of each marker varies 0.20-0.89 with an average of 0.53.…”

Analyse de la diversité génétique des isolats de <i>Magnaporthe oryzae</i> du Burkina Faso et du Togo par les marqueurs microsatellites (SSRs)

“…L'objectif est d'acquérir des données sur la structure génétique des isolats de M. oryzae collecté entre 2013 et 2015 au Burkina Faso et au Togo. Treize (13) marqueurs microsatellites ont été utilisés pour caractériser 220 isolats selon la méthode décrite par Adreit et al (2007). Les résultats révèlent un niveau de polymorphisme très élevé entre les isolats.…”

“…L'extraction ADN a été faite suivant la méthodologie décrite par Adreit et al (2007). Pour le génotypage, treize marqueurs microsatellites ou SSRs (Simple Sequence Repeats) définis par Kaye et al (2003) et Adreit et al (2007), ont été utilisés pour évaluer le niveau de polymorphisme des isolats étudiés (Tableau 1).…”

Phylogenetic diversity and sensitivity to MBI and QoI fungicides of <i>Magnaporthe oryzae</i> in Taiwan

“…14) Therefore, molecular markers were applied to study the population structure of P. oryzae in the field, 15) including MGR (Magnaporthe grisea repeat element), 10,16) MAGGY (M. gypsy-like element), 17,18) Pot2 (P. oryzae repeat 2) 19) and SSR (simple sequence repeat). 20) Many fungicides, including acid amides, aliphatic compounds, organo-phosphorus compounds, antibiotics, benzimidazoles, strobilurins, organo-sulphur compounds and heterocyclic compounds, are recommended for controlling rice blast. 21,22) Among these compounds, carpropamid of acid amides and tricyclazole of heterocyclic compounds are the most commonly used fungicides in Taiwan.…”