Magnaporthe oryzae (Anamorph: Pyricularia oryzae), the causal agent of rice blast disease, is the major pathogen that reduces the yield of rice worldwide. Melanin biosynthesis inhibitors (MBIs) and strobilurins (QoIs) are common fungicides used to control the disease. One hundred and three M. oryzae isolates from different rice-production areas in Taiwan were evaluated for their molecular phylogeny and sensitivity to MBI and QoI fungicides. Molecular phylogenetic analysis showed that all M. oryzae isolates could be classified into nine genetic groups (A-I) based on the DNA polymorphism amplified by CNS1/MP primers. Predominant ones are groups A (72 isolates) and B (15 isolates). Fungicide tests showed that M. oryzae isolates were sensitive to MBIs (carpropamid and tricyclazole) and less sensitive to QoIs (kresoxim-methyl and azoxystrobin). Amplifying the cytochrome b (cyt b) gene revealed that isolates with low sensitivity to QoIs did not correspond with mutation at codon 129 or 143 in the cyt b gene.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.