Microsatellite markers for Cattleya walkeriana Gardner, an endangered tropical orchid species

Tambarussi, Evandro Vagner; Menezes, Lou; Ibanes, Bruna; Antiqueira, Lia Maris Orth Ritter; Dequigiovanni, Gabriel; Moreno, Marlene Aparecida; Ferraz, Elza Martins; Zucchi, Maria Imaculada; Veasey, Elizabeth Ann; Vencovsky, Roland

doi:10.1017/s1479262115000635

Cited by 26 publications

(13 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA extraction, amplifications and microsatellite loci scoring steps were performed following Tambarussi et al (2017). Eight microsatellite loci (Cw01, Cw02, Cw03, Cw04, Cw05, Cw07, Cw08, and Cw09) specific for C. walkeriana were used (Tambarussi et al, 2017).…”

Section: Plant Materials and Microsatellite Analysismentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity between native and improved Cattleya walkeriana Gardner famous clones

et al. 2017

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The aim of this study was to evaluate the genetic diversity among native plants and some individuals obtained from crosses with unknown genealogy of C. walkeriana as well as C. loddigesii and C. nobilior and to advance towards solving the question of the genetic purity of the "Orchidglade" clone. Eight microsatellite loci were used to evaluate the genetic diversity between individuals of C. walkeriana. Microsatellites were not efficient in determining the genetic diversity between C. walkeriana groups (native and improved). The difficulty in determining the genetic distance between the different genotypes can be attributed to the complex mating system of the species and to a weak genetic barrier that facilitates the development of hybrids. Our analysis revealed smaller genetic distances between the "Orchidglade", "Equilab", "Kenny" and "Pedentive" clones and the species C. loddigesii and C. nobilior. Native C. walkeriana plants were genetically more distant from the C. loddigesii and C. nobilior species.Keywords: orchid improvement, genetic variation, genetic differentiation, Cattleya loddigesii, Cattleya nobilior.Diversidade genética entre clones famosos nativos e melhorados de Cattleya walkeriana Gardner RESUMO. O objetivo desta pesquisa foi o de avaliar a diversidade genética entre plantas nativas e indivíduos de genealogia desconhecida de C. walkeriana, bem como C. loddigesii e C. nobilior, e também avançar na solução do dilema da origem do clone "Orchidglade" de C. walkeriana. Oito locos microssatélites foram utilizados para avaliar a diversidade genética entre indivíduos de C. walkeriana. Os marcadores microssatélites não foram eficientes na determinação da diversidade genética entre os grupos C. walkeriana (nativas e melhoradas). A dificuldade em determinar a distância genética entre os genótipos diferentes pode ser devida a um sistema complexo de reprodução das espécies e devido a uma fraca barreira reprodutiva facilitando o desenvolvimento de híbridos. Nossa análise revelou menores distâncias genéticas entre os clones Orchidglade, "Equilab", "Kenny" e "Pedentive" e as espécies C. loddigesii e C. nobilior. As C. walkeriana nativas se mostraram geneticamente mais distantes das espécies de C. loddigesii e C. nobilior. Palavras-chave: melhoramento de orquídeas, variação genética, diferenciação genética, Cattleya loddigesii, Cattleya nobilior.

show abstract

Section: Plant Materials and Microsatellite Analysismentioning

confidence: 99%

“…Molecular markers have been used for the genetic analysis of many orchids, such as Cypripedium and Calanthe (Qian, Wang, & Tian, 2013), Cattleya (Almeida et al, 2013;Novello et al, 2013;Tambarussi et al, 2017), and Liparis (Broeck et al, 2014), among others.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity between native and improved Cattleya walkeriana Gardner famous clones

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Conserving such hereditary legacy is crucial for long-term species survival (Muñoz, Warner, & Albertazzi, 2010). Cattleya walkeriana Gardner is now a threatened species due to forest fragmentation and predatory collection (Tambarussi et al, 2017). Growers appreciate C. walkeriana due to its diversity of forms, springing in beautiful and valuable flowers (Faria, Santiago, Saridakis, Albino, & Araújo, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, collectors have been looking for plants with high genetic improvement (Menezes, 2011). Individuals with improved traits (rare color and good flower shape) are highly valued (Tambarussi et al, 2017). Cattleya loddigesii Lindl.…”

Section: Introductionmentioning

confidence: 99%

DNA barcode regions for differentiating Cattleya walkeriana and C. loddigesii

Rivera-Jiménez

Rossini

Tambarussi

et al. 2017

Acta Sci. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Growers appreciate Cattleya walkeriana and C. loddigesii due to striking shape and rarity. Thus, this study aimed to evaluate the feasibility of DNA barcode regions, namely ITS1, ITS2 and rpoC1, to discriminate between C. walkeriana and C. loddigesii species. DNA barcode regions were successfully amplified using primers designed to amplify plants. We also included sequences from public databases in order to test if these regions were able to discriminate C. walkeriana and C. loddigesii from other Cattleya species. These regions, and their combinations, demonstrated that the ITS1+ITS2 had the highest average interspecific distance (11.1%), followed by rpoC1 (1.06%). For species discrimination, ITS1+ITS2 provided the best results. The combined data set of ITS1+ITS2+rpoC1 also discriminated both species, but did not result in higher rates of discrimination. These results indicate that ITS region is the best option for molecular identification of these two species and from some other species of this genus.Keywords: orchid improvement, genetic variation, species separation.Regiões de DNA barcode para diferenciar Cattleya walkeriana e C. loddigesii RESUMO. As espécies Cattleya walkeriana e C. loddigesii são apreciadas pelos colecionadores devido às suas impressionantes forma e raridade. Este estudo teve como objetivo avaliar a viabilidade das regiões DNA barcode, ou seja, ITS1, ITS2 e rpoC1, para discriminar as espécies C. walkeriana e C. loddigesii. Regiões DNA barcode foram amplificadas com êxito utilizando os iniciadores desenhados para plantas. Nós também incluímos sequências de bases públicas de dados, a fim de testar se estas regiões foram capazes de discriminar C. walkeriana e C. loddigesii de outras espécies de Cattleya. Estas regiões e suas combinações demonstraram que o ITS1 + ITS2 teve a maior distância média interespecífica (11,1%), seguido por rpoC1 (1,06%). Para a discriminação das espécies, ITS1 + ITS2 proporcionaram os melhores resultados. Os dados combinados dos ITS1 + ITS2 + rpoC1 também discriminaram ambas as espécies, mas não resultaram em maiores taxas de discriminação. Estes resultados indicam que a região ITS é a melhor opção para a identificação molecular destas duas espécies e a partir de algumas outras espécies deste gênero.Palavras-chave: melhoramento de orquídeas, variação genética, separação de espécies.

show abstract

“…LMOR Antiqueira et al (Almeida et al 2013, Novello et al 2013, Tambarussi et al 2016, among others. However, there are no previous reports of genetic studies for T. pumilum and no information on the level of genetic diversity of its populations.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum

Antiqueira

Dequigiovanni

Tambarussi³

et al. 2016

Crop Breed. Appl. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Microsatellite markers for Cattleya walkeriana Gardner, an endangered tropical orchid species

Cited by 26 publications

References 11 publications

<b>Genetic diversity between native and improved <i>Cattleya walkeriana</i> Gardner famous clones

<b>Genetic diversity between native and improved <i>Cattleya walkeriana</i> Gardner famous clones

<b>DNA barcode regions for differentiating <i>Cattleya walkeriana</i> and <i>C. loddigesii</i>

Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum

Contact Info

Product

Resources

About