Microsatellite locus development in the seaweed <i>Plocamium</i> sp.

Heiser, Sabrina; Amsler, Charles D.; Krueger‐Hadfield, Stacy A.

doi:10.1017/s0954102022000475

Cited by 2 publications

(6 citation statements)

References 18 publications

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“…from around the study area. The presence of cystocarpic female gametophytes (evidence of syngamy) and reproductive tetrasporophytes (evidence of meiosis) at sites near Anvers Island suggests that sexual reproduction occurs, though many thalli at the time of collection in our previous studies from there were vegetative, and we do not know the life cycle stage (Heiser, Amsler, & Krueger‐Hadfield, 2023; Heiser, Shilling, et al, 2023). Recently, we developed microsatellite loci (Heiser, Amsler, & Krueger‐Hadfield, 2023), and we used the loci here to describe the prevailing reproductive system in Plocamium sp.…”

Section: Introductionmentioning

confidence: 80%

“…The presence of cystocarpic female gametophytes (evidence of syngamy) and reproductive tetrasporophytes (evidence of meiosis) at sites near Anvers Island suggests that sexual reproduction occurs, though many thalli at the time of collection in our previous studies from there were vegetative, and we do not know the life cycle stage (Heiser, Amsler, & Krueger‐Hadfield, 2023; Heiser, Shilling, et al, 2023). Recently, we developed microsatellite loci (Heiser, Amsler, & Krueger‐Hadfield, 2023), and we used the loci here to describe the prevailing reproductive system in Plocamium sp. at 12 sites near Anvers Island.…”

Section: Introductionmentioning

confidence: 80%

“…We used the 10 microsatellite loci described in Heiser, Amsler, and Krueger‐Hadfield, (2023). We amplified loci for some thalli in simplex PCRs with a final volume of 10 μL: 2 μL of DNA, forward and reverse primers (see Table S2 in the Supporting Information), 1X Promega GoTaq® Flexi Buffer (Promega), 2 mM of MgCl 2 , 250 μM of each dNTP (Promega), and 0.5 U of Promega GoTaq® Flexi DNA Polymerase.…”

Section: Methodsmentioning

confidence: 99%

“…Information on poolplexes for fragment analysis is provided in Table S2. Alleles were scored following Heiser, Amsler, and Krueger‐Hadfield, (2023) using Geneious Prime (Biomatters, Ltd.). We followed Heiser, Amsler, and Krueger‐Hadfield, (2023) to account for any base pair shifts between different dyes and size ladders.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we developed microsatellite loci (Heiser, Amsler, & Krueger-Hadfield, 2023), and we used the loci here to describe the prevailing reproductive system in Plocamium sp. at 12 sites near Anvers Island.…”

Section: Introductionmentioning

confidence: 99%

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Tetrasporophytic bias coupled with heterozygote deficiency in Antarctic Plocamium sp. (Florideophyceae, Rhodophyta)

Heiser

Amsler

Stoeckel

et al. 2023

Journal of Phycology

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Meiosis and syngamy generate an alternation between two ploidy stages, but the timing of these two processes varies widely across taxa, thereby generating life cycle diversity. One hypothesis suggests that life cycles with long‐lived haploid stages are correlated with selfing, asexual reproduction, or both. Though mostly studied in angiosperms, selfing and asexual reproduction are often associated with marginal habitats. Yet, in haploid‐diploid macroalgae, these two reproductive modes have subtle but unique consequences whereby predictions from angiosperms may not apply. Along the western Antarctic Peninsula, there is a thriving macroalgal community, providing an opportunity to explore reproductive system variation in haploid‐diploid macroalgae at high latitudes where endemism is common. Plocamium sp. is a widespread and abundant red macroalga observed within this ecosystem. We sampled 12 sites during the 2017 and 2018 field seasons and used 10 microsatellite loci to describe the reproductive system. Overall genotypic richness and evenness were high, suggesting sexual reproduction. Eight sites were dominated by tetrasporophytes, but there was strong heterozygote deficiency, suggesting intergametophytic selfing. We observed slight differences in the prevailing reproductive mode among sites, possibly due to local conditions (e.g., disturbance) that may contribute to site‐specific variation. It remains to be determined whether high levels of selfing are characteristic of macroalgae more generally at high latitudes, due to the haploid‐diploid life cycle, or both. Further investigations of algal life cycles will likely reveal the processes underlying the maintenance of sexual reproduction more broadly across eukaryotes, but more studies of natural populations are required.

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Section: Introductionmentioning

confidence: 80%