Microsatellite loci in the cactophilic species <i>Drosophila antonietae</i> (Diptera; Drosophilidae)

Machado, Luciana Paes de Barros; Manfrin, Maura Helena; Silva-Júnior, Wilson A.; Sene, Fábio de Melo

doi:10.1046/j.1471-8286.2003.00381.x

Cited by 7 publications

(14 citation statements)

References 3 publications

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“…The total DNA of each ethanol‐preserved fly was isolated using a Promega DNA extraction kit. Eight microsatellite loci were used in the genetic analysis; three were developed for D. antonietae (HaeIIIanto‐3, HaeIII400anto‐5 and AluRSAIanto‐6; Machado et al, 2003) and five for D. buzzatii (M290, M122, M142, M411 and M223; Frydenberg et al 2002). Drosophila antonietae and D. buzzatii belong to the D. buzzatii species cluster, to which D. gouveai is taxonomically allocated (Tidon‐Sklorz and Sene 2001).…”

Section: Methodsmentioning

confidence: 99%

“…Drosophila antonietae and D. buzzatii belong to the D. buzzatii species cluster, to which D. gouveai is taxonomically allocated (Tidon‐Sklorz and Sene 2001). The loci HaeIIIanto‐3 and AluRSAIanto‐6 are compound dinucleotide repeats, whereas HaeIII400anto‐5 is a pure trinucleotide repeat (Machado et al 2003), and M290, M122, M142, M411 and M223 are all pure dinucleotide repeats (Frydenberg et al 2002). Although the chromosomal locations of these eight loci are unknown, they were considered to be autosomal‐linked since heterozygote genotypes were observed in both sexes, as also seen in the species from which they were originally isolated (Frydenberg et al 2002; Machado et al 2003).…”

Section: Methodsmentioning

confidence: 99%

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Microsatellite and morphometric variation in Drosophila gouveai: the relative importance of historical and current factors in shaping the genetic population structure

Moraes

Sene

2007

J Zoological System

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Drosophila gouveai is a cactus-breeding species with a naturally fragmented distribution in central-western and southeastern Brazil. In this study, a neutral genetic marker (microsatellite DNA) and a quantitative trait (wing morphology) were used to investigate the population structure of eight populations of D. gouveai. Quantitative analysis was done using a morphometric approach and multivariate analysis of 17 wing parameters. Drosophila gouveai showed a large degree of genetic population differentiation (F ST ¼ 0.245). A molecular analysis of variance showed that the population structure was attributable mainly to genetic differences among the population groups, which were correlated with regional groupings. A Bayesian cluster analysis also identified the same regional groupings as contributing to the population structure. Assignment tests revealed that the current gene flow was very restricted. The divergence in wing morphology among populations was also high, and revealed a geographical pattern that conformed to a latitudinal cline. In contrast to current factors such as migration-drift equilibrium, these results indicated that the genetic population structure of D. gouveai was shaped predominantly by historical factors.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Microsatellite and morphometric variation in Drosophila gouveai: the relative importance of historical and current factors in shaping the genetic population structure

Moraes

Sene

2007

J Zoological System

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“…Following the protocol proposed by Mateus et al (2005), thorax and abdomen were individually dry stored at -20ºC and used for the allozymic analyses in another work (Lorenci et al, 2010). The respective heads were stored in 70% ethanol at -20ºC and were used for DNA extraction, which were amplified for 7 microsatellite loci (AluRSAIanto-1, HaeIIIanto-2, HaeIIIanto-3, HaeIII400anto-4, HaeIII400anto-5, AluRSAIanto-6, AluRSAIanto-7), according to Machado et al (2003).…”

Section: Speciesmentioning

confidence: 99%

“…The amplification of the seven microsatellite loci described by Machado et al (2003) was conducted in 75 Drosophila antonietae specimens (25 from each natural population analyzed). The HaeIIIanto-2 locus was amplified only in four of these samples, two from Cantagalo and two from Rio do Poço.…”

Section: Environment 1: Xerophytic Vegetation Islandsmentioning

confidence: 99%

Spatial Variation of Genetic Diversity in Drosophila Species from Two Different South American Environments

Machado¹,

Silva²,

Simão³

et al. 2012

Analysis of Genetic Variation in Animals

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“…Some works have suggested that the population structure of some species of the D. buzzatii cluster can be defined by the occurrence of gene flow, causing little population structure, or due to geographic isolation, suggesting isolated populations in models of islands (Moraes & Sene, 2002;Machado et al, 2003;Mateus et al, 2003). These population studies suggest that recurrent events determine the distribution of the variation between the populations of the D. buzzatii cluster.…”

Section: Evolutionary History -Concluding Remarksmentioning

confidence: 99%

Cactophilic Drosophila in South America: A Model for Evolutionary Studies

Manfrin¹,

Sene²

2006

Genetica

134

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The Drosophila buzzatii cluster is composed of seven cactophilic species and their known geographical distribution encompasses the open vegetation diagonal, which includes the morphoclimatic Domains of the Caatinga, Chaco and Cerrado, which are situated between the Amazon and the Atlantic forests. Besides these areas, these cactophilic species are also found in a narrow strip along the Atlantic coast from northeastern Brazil to the southern tip of the country. The hypothesis of vicariant events, defining the core areas of each species, is proposed to explain the historical diversification for the cluster. The intraspecific analysis for the cluster shows a population structure with gene flow restricted by distance, range expansion with secondary contact resulting in introgression and simpatry, especially in the limits of the species distribution, polytypic populations and assortative mating in inter population experiments. There is a variation related to these events that depends on the species and geographic origin of the population analyzed. These events are, hypothetically, described as the results of expansion and retraction of the population ranges, as a consequence of their association with cacti, which theoretically follow the expansion and retraction of dry areas during the paleoclimatic oscillations in South America, as that promoted by the glacial cycles of the Quaternary. The Drosophila buzzatii cluster is divided into two groups. The first one is composed of D. buzzatii, a species that has a broad geographic distribution and no significant differentiation between its populations. The second is the Drosophila serido sibling set, which encompasses the others species and is characterized by a significant potential for differentiation.

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Microsatellite loci in the cactophilic species Drosophila antonietae (Diptera; Drosophilidae)

Cited by 7 publications

References 3 publications

Microsatellite and morphometric variation in Drosophila gouveai: the relative importance of historical and current factors in shaping the genetic population structure

Microsatellite and morphometric variation in Drosophila gouveai: the relative importance of historical and current factors in shaping the genetic population structure

Spatial Variation of Genetic Diversity in Drosophila Species from Two Different South American Environments

Cactophilic Drosophila in South America: A Model for Evolutionary Studies

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