Microsatellite Heterozygosity: Functional Constraints on the Development and Use of Comprehensive Genomic Maps for Livestock

Beattie, Craig W.

doi:10.1007/978-1-4899-0280-1_2

Cited by 2 publications

(3 citation statements)

References 61 publications

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“…These tandemly repeated, highly polymorphic, single-locus DNA sequences (Weber 1990) are well distributed over numerous mammalian genomes (Hamada et al 1982;Tautz 1989;Weber & May 1989), and are readily adaptable to polymerase chain reaction (PCR) systems (Weber & May 1989). Microsatellites are currently the marker of choice for establishing linkage maps (Dietrich et al 1994;Barendse et al 1994;Bishop et al 1994;Crawford et al 1995;Hudson et al 1995), estimating genetic diversity (Takezaki & Nei 1996) and studying evolutionary relationships (reviewed in Beattie 1996). Primer pairs developed for ms loci derived from cattle and sheep have recently been reciprocally assigned to linkage groups on homologous chromosomes within each species Bishop et al 1994;Crawford et al 1995;Barendse et al 1997;Kappes et al 1997a).…”

Section: Introductionmentioning

confidence: 99%

Extensive genomic conservation of cattle microsatellite heterozygosity in sheep

et al. 1997

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We report the evaluation of 1036 bovine microsatellite primer pairs for their suitability as linkage markers in sheep. Approximately 58% (605/1036) of bovine primer pairs amplified a locus in sheep. Sixty-seven per cent (409/605) of amplified loci were detected as polymorphic. Marker heterozygosity, allele number and range of allele sizes were significantly lower in sheep than cattle sampled in this study. However, median fragment size was similar. These data suggest that high-resolution comparative linkage maps between closely related species can be constructed relatively efficiently.

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Section: Introductionmentioning

confidence: 99%

Extensive genomic conservation of cattle microsatellite heterozygosity in sheep

et al. 1997

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“…The observed heterozygosity values for the 21 loci ranged from 0.636(ETH3) to 0.863(SPS115) with a mean of 0.801±0.019 (Table2 and Figure 3) and similarly, the highest expected heterozgosity was observed at SPS115 (0.863) and lowest at ETH3 (0.636) with an overall mean of 0.768±0.013 in Jersey crossbred cattle population of Tamil Nadu used in this study. According to Beattie (1996)…”

Section: Heterozygositymentioning

confidence: 99%

“…Polymorphism information content (PIC) 27 of the microsatellite loci in this study ranged from 0.592(ETH3) to 0.830 (CSSM66) with an overall mean of 0.726 ± 0.02 (Table2and Figure 3) indicating that markers used in the study are highly polymorphic and suitable in assigning the parentage. Informativeness (polymorphism) represents by the probability that a given offspring of a parent carrying the rare allele allows deduction of the parental genotype at the marker locus 36 . Comparable PIC values were observed in Czech Holstein Friesian population ranging from 0.575 (SPS115, 8 alleles) to 0.816 (TGLA227, 12 alleles) with the average value of 0.713 6 .…”

Section: Polymorphism Information Content (Pic)mentioning

confidence: 99%

Assessment of Microsatellite Markers for Parentage Testing in Jersey Crossbred Cattle in Tamil Nadu

Hepsibha

Karthickeyan

Sumathy

2022

Int J Life Sci Pharm Res

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A study was aimed to evaluate a set of polymorphic microsatellite markers for their efficiency in testing parentage in Jersey crossbred cattle available in Tamil Nadu. The objective of validation was based on the criteria such as polymorphism information content, various genetic diversity parameters and the ability of markers to exclude the wrong parent. A total of 21 microsatellite markers were included to verify the parentage in 24 Jersey crossbred trios (comprising 24 dams, 24 progenies and 2 sires). The microsatellite loci were amplified using fluorescently labelled primers by multiplex PCR and fragment analysis was done through capillary electrophoresis using automated DNA analyzer. Statistical analyses were done using Pop gene version 1.31, CERVUS 3.0.7 and GENALEX 6.503 software programs. The number of alleles for the markers utilized in the study ranged from 4 (ILSTS11) to 12 (INRA23 and TGLA122) with an overall mean of 8.0952 ± 0.47 alleles per locus. The markers also exhibited high expected heterozygosity (He) and polymorphism information content (PIC) ranging from 0.6362 (ETH3) to 0.8629 (SPS115) with a mean of 0.7681 ± 0.013 and 0.592(ETH3) to 0.83(CSSM66) with a mean of 0.7256±0.014 respectively, signifying them to be highly informative. Low overall probability of identity (PI) of 0.0943 ± 0.008 and high overall probability of exclusion (PE) of 0.9619±0.02 with the increasing locus combinations were observed. The probability of exclusion was cent per cent (PE=1.0000) when a combination of 8 markers were used with one known parent and a combination of 12 markers when excluding a putative pair, suggesting the efficacy and suitability of the markers used in the study for parentage testing on Jersey crossbreds of Tamil Nadu.

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Microsatellite Heterozygosity: Functional Constraints on the Development and Use of Comprehensive Genomic Maps for Livestock

Cited by 2 publications

References 61 publications

Extensive genomic conservation of cattle microsatellite heterozygosity in sheep

Extensive genomic conservation of cattle microsatellite heterozygosity in sheep

Assessment of Microsatellite Markers for Parentage Testing in Jersey Crossbred Cattle in Tamil Nadu

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