Microsatellite DNA markers revealed genetic population structure among captive stocks and wild populations of mrigal, Cirrhinus cirrhosus in Myanmar

Aung, Ohnmar; Nguyen, Thuy Thi Thu; Poompuang, Supawadee; Kamonrat, Wongpathom

doi:10.1016/j.aquaculture.2009.12.010

Cited by 20 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The deviation from Hardy-Weinberg equilibrium observed for Borg10 and Borg54 can be attributed to the presence of null alleles at these loci, which might have led to the erroneous detection of homozygous genotypes and thus the underestimation of heterozygotes (Aung et al 2010). In addition, linkage disequilibrium showed genetic drift and suggested deviation from the Hardy-Weinberg equilibrium, particularly when considering small populations, where the effect of drift accentuates random changes in allele frequencies over time (Frankham et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Souza

Urrea-Rojas

Ruas

et al. 2018

Italian Journal of Animal Science

View full text Add to dashboard Cite

Brycon orbignyanus is a neotropical fish found in several South American countries. This species has high ecological importance and is included in the list of fish threatened with extinction. In this study, we report the development and characterisation of 12 microsatellite primers of B. orbignyanus for future genetic studies. Screening of the 12 markers in a sample of 35 individuals identified seven polymorphic loci and 22 alleles, with one to three alleles per locus. The polymorphic information content ranged from 0.054 to 0.542, with four loci showing significant deviation from Hardy-Weinberg equilibrium. Two loci possessed null alleles. Cross-species amplification tests in two closely related species showed that 11 loci are transferrable to Brycon gouldingi (B. gouldingi) and nine are transferrable to B. falcatus. The microsatellite primers designed may be used for genetic variability and population structure studies in wild populations, fish farms and restocking programmes of B. orbignyanus and the two related species. ARTICLE HISTORY

show abstract

Section: Discussionmentioning

confidence: 99%

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Souza

Urrea-Rojas

Ruas

et al. 2018

Italian Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…Endogamy was observed in PRO1, BAB1, AGV2, NAV2, IBI2, BAR2 and BAB2. The deficit of heterozygotes at one or more loci and losses of alleles might indicate the presence of null alleles (AUNG et al, 2010), the Wahlund effect, or both (HATANAKA et al, 2006), or the bottleneck effect (GONZÁLEZ-WANGUEMERT et al, 2012). The Wahlund effect did not promote endogamy because the samples were collected in geographically separate locations.…”

Section: Genetic Variabilitymentioning

confidence: 99%

“…Genetic diversity of pacu for restocking programs in the Tietê and Grandes rivers, Brazil been useful for addressing questions relative to the population diversity and genetic structure of various fish species (NOLAN;POWELL, 2009;AUNG et al, 2010;HULAK et al, 2010;HORNE et al, 2013;MERANER et al, 2013). The objective of the current, unprecedented study was to evaluate the genetic diversity of P. mesopotamicus WPs and BSs using microsatellites markers for restocking programs in the Tietê and Grande rivers (Brazil).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of pacu for restocking programs in the Tietê and Grande rivers, Brazil

Ribeiro

Lopera-Barrero

Santos³

et al. 2015

Sem. Ci. Agr.

View full text Add to dashboard Cite

Piaractus mesopotamicus is a tropical fish under threat because of declines in the number of wild populations. Restocking programs have been developed as a conservation method, and the genetic monitoring of populations and broodstocks is important to ensure the viability of such programs. The objective of the current unprecedented experiment was to evaluate the genetic diversity of P. mesopotamicus wild populations (WPs) and broodstocks (BSs) using microsatellites markers for supplying the restocking programs in the Tietê and Grande rivers. Six microsatellite loci were amplified using the DNA obtained from the caudal fin of 279 adult fish. The observed intrapopulation genetic variability was high with mean heterozygosity ranging from 0.203 to 0.833. The number of alleles per locus ranged from three (loci Pme28 and Pme32) to thirteen (loci Pme4, Pme5 and Pme14), and there were allele differences between WPsxWPs and WPsxBSs. This differentiation was confirmed by the dendrogram analysis that showed three specific clusters. Four alleles were shared in the WPs2012xBSs. Positive FIS values indicated the presence of endogamy in seven out of ten samples obtained from the WPs. The AMOVA analysis and FST values indicated moderate and high genetic differences in WPsxWPs and high genetic differences in WPsxBSs. The genetic distance and genetic identity values and number of migrants confirmed these results. Adequate intrapopulation genetic variability, similarity between BSsxBSs, and genetic differences between WPs2011xWPs2012 and WPsxBSs were observed. In the wild, individuals from the restocking program were partially observed. ResumoPiaractus mesopotamicus é um peixe tropical que nos últimos anos tem apresentado uma diminuição no número de populações naturais. Programas de repovoamento vêm sendo utilizados como método de conservação, entretanto, o monitoramento genético das populações e dos estoques de reprodutores é importante para conferir a viabilidade desse tipo de programas. O objetivo do presente estudo foi avaliar de forma inédita a diversidade genética de populações selvagens (WPs) e estoques de reprodutores (BSs) de P. mesopotamicus utilizados em programas de repovoamento dos rios Tietê e Grande, através de marcadores microssatélite. Seis loci microssatélite foram amplificados usando DNA extraído de nadadeira caudal de 279 indivíduos adultos. Foi observada alta variabilidade genética intrapopulacional, com medias de heterozigosidade observada entre 0.203 e 0.833. O número de alelos por locus foi de três (locus Pme28 e Pme32) a 13 (locus Pme4, Pme5 e Pme14) e houve diferenciação de alelos entre WPsxWPs e WPsxBSs. Essa diferenciação foi confirmada pela análise do dendrograma que mostrou a formação de três agrupamentos específicos. Observaram-se quatro alelos compartilhados entre WPs2012xBSs. Valores positivos de F IS mostraram a presença de endogamia em sete das 10 coletas realizadas nas WPs. A análise de AMOVA e do F ST indicou moderada e muito alta diferenciação genética entre WPsxWPs e diferenciação gen...

show abstract

“…The heterozygote deficit can appear due to the presence of null alleles (Aung, Nguyen, Poompuang, & Kamonrat, ), the Wahlund effect, or a combination of both (Hatanaka, Henrique‐Silva, & Galetti, ), due to inbreeding (O'Connell & Wright, ), or as a consequence of the bottleneck effect (González‐Wangüemert et al, ). The analysis carried out using the Micro‐Checker software did not indicate the presence of a large number of null alleles, ruling out their interference.…”

Section: Discussionmentioning

confidence: 99%

Structure and genetic variability of golden mussel (Limnoperna fortunei) populations from Brazilian reservoirs

Furlan-Murari

Ruas

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

The golden mussel, Limnoperna fortunei a highly invasive species in Brazil, has generated productive, economical, and biological impacts. To evaluate genetic structure and variability of L. fortunei populations present in fish farms in the reservoirs of Canoas I (CANFF), Rosana (ROSFF), and Capivara (CAPFF) (Paranapanema River, Paraná, Brazil), eight microsatellite loci were amplified. Five of those eight loci resulted in 38 alleles. The observed heterozygosity (Ho) was lower than the expected heterozygosity (He) in all populations, with a deviation from the Hardy–Weinberg equilibrium (HWE). The average value for the inbreeding coefficient (Fis) was positive and significative for all populations. There was higher genetic variability within populations than among them. The fixation index (Fst) showed a small genetic variability among these populations. The occurrence of gene flow was identified in all populations, along with the lack of a recent bottleneck effect. The clustering analysis yielded K = 2, with genetic similarity between the three populations. The results demonstrate low genetic structure and suggest a founding population with greater genetic variability (ROSFF). Our data point to the possible dispersal of L. fortunei aided by anthropic factors in the upstream direction. It was concluded that the three populations presented a unique genetic pool for Paranapanema River, with occurrence of gene flow.

show abstract

Microsatellite DNA markers revealed genetic population structure among captive stocks and wild populations of mrigal, Cirrhinus cirrhosus in Myanmar

Cited by 20 publications

References 23 publications

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Genetic diversity of pacu for restocking programs in the Tietê and Grande rivers, Brazil

Structure and genetic variability of golden mussel (Limnoperna fortunei) populations from Brazilian reservoirs

Contact Info

Product

Resources

About