Microsatellite DNA Analysis for Diversity Study, Individual Identification and Parentage Control in Pig Breeds in Poland

Abstract

Swine DNA profiling is of high importance for animal identification and parentage verification. The aim of this study was to test a set of 14 microsatellite (STR) markers recommended by ISAG for parentage verification in Polish Landrace (PL, n = 900), Polish Large White (PLW, n = 482), Pulawska (PUL, n = 127), and Duroc pigs (DU n = 108). The studied breeds showed a medium level of genetic differentiation. The average value of heterozygosity and degree of polymorphism (PIC) were above 0.5 for the studied breeds, except for the DU breed (PIC = 0.477). The population inbreeding coefficient indicates an absence of inbreeding in the studied breeds (an average value of FIS = 0.007). The cumulative power of discrimination for all breeds reached high values close to 1.0, while the probability of identity (PID) was low, with PID values ranging between 10−9 (for DU) and 10−12 (for PLW). The cumulative exclusion probability for PE1 and PE2 showed that the parentage can be confirmed with a probability of from 92.75% to 99.01% and from 99.49% to 99.97%, respectively.

Microsatellite-Based Genetic Structure and Hybrid Detection in Alpacas Bred in Poland

“…In the present study, the CPE1 was 0.99995 and CPE2 0.99999, which is higher than that obtained for alpacas [ 59 ], llamas and guanacos [ 66 ]. In the latest studies on animals bred in Poland, namely horses ( Equus caballus ) [ 67 ], pigs ( S. domestica ) [ 68 ] and dogs ( C. familiaris ) [ 69 ], lower values were also obtained. The results obtained for alpacas illustrate the utility of the tested markers for parentage testing.…”

Current Analytical Methods and Research Trends Are Used to Identify Domestic Pig and Wild Boar DNA in Meat and Meat Products

“…Short tandem repeats (STRs, also known as microsatellites) are genetic markers that are commonly used in parentage testing and verification [ 73 ]. These markers are specific for the species, so they seemed good candidates to distinguish wild boar and pig.…”