Current Analytical Methods and Research Trends Are Used to Identify Domestic Pig and Wild Boar DNA in Meat and Meat Products

Natonek-Wiśniewska, Małgorzata; Piestrzyńska-Kajtoch, Agata; Koseniuk, Anna; Krzyscin, P.

doi:10.3390/genes13101825

Cited by 6 publications

(3 citation statements)

References 86 publications

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“…It should be noted that in the event that one needs to detect several species simultaneously in one reaction, more than one digestion enzyme usually needs to be used. Nevertheless, the method itself is qualitative, very sensitive, quite cost-effective and has been successfully applied to various mixed products, which is why it is still used as the preferred method in various laboratories (Table 2) [22].…”

Section: Polymerase Chain Reaction-restriction Fragment Length Polymo...mentioning

confidence: 99%

Tracing the Domestic Pig Using the Omics Technologies

Djurkin Kušec,

Gvozdanović

2024

Tracing the Domestic Pig

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Pork represents one of the most important sources of protein in the human diet. Consumers today expect their food to be safe and of expected quality. Therefore, traceability and originality of the product must be guaranteed. This chapter provides an overview of the different approaches used for traceability and authentication of pork and pork products. Different DNA-based methods for meat speciation and authentication are described and their potential for use in the pork industry is highlighted.

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Section: Polymerase Chain Reaction-restriction Fragment Length Polymo...mentioning

confidence: 99%

Tracing the Domestic Pig Using the Omics Technologies

Djurkin Kušec,

Gvozdanović

2024

Tracing the Domestic Pig

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“…Microsatellite markers are simple sequence repeats of highly polymorphic and codominant DNA motifs (n could range from 8 to 50) that are widely distributed throughout the genomes of eukaryotes and are widely used for different population genetic studies in many different species, such as genetic diversity [ 27 , 28 , 29 ], population structure, parentage determination [ 30 , 31 , 32 ], and constructing genetic linkage maps [ 33 , 34 , 35 , 36 , 37 ], animal identification, meat traceability [ 38 , 39 ] and animal breeding [ 40 , 41 ]. Studies on related aquatic species with highly duplicated genomes have demonstrated that microsatellites derived from transcriptomes are significantly more effective than those developed from the genome using traditional methods [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Yang,

Chu,

Chen

et al. 2024

Animals

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The Pacific blue shrimp (Litopenaeus stylirostris) is a premium product in the international seafood market. However, intensified farming has increased disease incidence and reduced genetic diversity. In this study, we developed a transcriptome database for L. stylirostris and mined microsatellite markers to analyze their genetic diversity. Using the Illumina HiSeq 4000 platform, we identified 53,263 unigenes from muscle, hepatopancreas, the intestine, and lymphoid tissues. Microsatellite analysis identified 36,415 markers from 18,657 unigenes, predominantly dinucleotide repeats. Functional annotation highlighted key disease resistance pathways and enriched categories. The screening and PCR testing of 42 transcriptome-based and 58 literature-based markers identified 40 with successful amplification. The genotyping of 200 broodstock samples revealed that Na, Ho, He, PIC, and FIS values were 3, 0.54 ± 0.05, 0.43 ± 0.09, 0.41 ± 0.22, and 0.17 ± 0.27, respectively, indicating moderate genetic variability and significant inbreeding. Four universal microsatellite markers (CL1472.Contig13, CL517.Contig2, Unigene5692, and Unigene7147) were identified for precise diversity analysis in Pacific blue, Pacific white (Litopenaeus vannamei), and black tiger shrimps (Penaeus monodon). The transcriptome database supports the development of markers and functional gene analysis for selective breeding programs. Our findings underscore the need for an appropriate genetic management system to mitigate inbreeding depression, reduce disease susceptibility, and preserve genetic diversity in farmed shrimp populations.

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“…Mislabeling and fraud meat processed products harm and decrease consumer trust [1]. The adulteration of pork or wild boar meat in food concerns researchers to obtain the appropriate and accurate methods for identifying both [2]. In Western societies, pork is frequently consumed as an animal protein source.…”

Section: Introductionmentioning

confidence: 99%

Advancements in DNA analysis for distinguishing wild boar and domestic pig: a mini-review on research developments for food authentication studies

Volkandari,

Rohman,

Jenie

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Ensuring food authenticity is integral to quality control and food safety, demanding traceability of food ingredient origins to be prominently featured on labels. Pork, a widely consumed and processed animal protein source, is susceptible to adulteration with wild boar meat, primarily driven by factors such as production costs and profitability. Complicating matters, the quality of wild boar meat obtained through game hunting lacks guarantees for hygienic standards, posing potential harm to consumers. Recent outbreaks of African Swine Fever in pigs and wild boars further emphasize the critical link between food safety and meat authenticity. This study addresses the need for research in distinguishing between pork and wild boar meat to uphold food quality control standards. Establishing the meat origin is not only crucial for quality control but also vital for mapping and tracing meat distribution patterns. The study adopts a DNA-based approach, utilizing biomarkers to distinguish domestic pig and wild boar meat. However, researchers encounter significant challenges due to their shared species (Sus scrofa) and high DNA sequence similarity. The pursuit of robust biomarkers and analytical methods becomes imperative. Exploring both nuclear and mitochondrial DNA, this mini-review outlines the research developments in identifying resilient biomarkers and employing various analytical methods, including conventional PCR, PCR-RFLP, Real-time PCR, microsatellite (STR), and deep sequencing (NGS or GWAS). The paper provides a concise overview of the ongoing efforts to find effective biomarkers and analytical methods based on DNA approaches for distinguishing domestic pigs and wild boars, showcasing their potential applications in food authentication.

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Current Analytical Methods and Research Trends Are Used to Identify Domestic Pig and Wild Boar DNA in Meat and Meat Products

Cited by 6 publications

References 86 publications

Tracing the Domestic Pig Using the Omics Technologies

Tracing the Domestic Pig Using the Omics Technologies

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Advancements in DNA analysis for distinguishing wild boar and domestic pig: a mini-review on research developments for food authentication studies

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