Microsatellite analysis to estimate genetic relationships among five bulgarian sheep breeds

Kusza, Szilvia; Dimov, D.; Nagy, István; Bösze, Zsuzsanna; Jávor, András; Kukovics, S.

doi:10.1590/s1415-47572010005000003

Cited by 17 publications

(24 citation statements)

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“…The values of genetic diversity parameters were higher compared with similar study of Tsigai and Zackel type group sheep breeds from central, eastern and southern European regions (Kusza et al, 2008). Also, the mean H e value of all 18 loci (0.844) was higher than the values reported in the literature (Kusza et al, 2008(Kusza et al, , 2010(Kusza et al, , 2011Neubauer et al, 2015). Positive values of F is indicate loss of heterozygosity in all loci, similar with the results reported by Kusza et al (2008Kusza et al ( , 2011, Kevorkian et al (2010) and Zahan et al (2011).…”

Section: Discussionsupporting

confidence: 84%

“…Also, the mean H e value of all 18 loci (0.844) was higher than the values reported in the literature (Kusza et al, 2008(Kusza et al, , 2010(Kusza et al, , 2011Neubauer et al, 2015). Positive values of F is indicate loss of heterozygosity in all loci, similar with the results reported by Kusza et al (2008Kusza et al ( , 2011, Kevorkian et al (2010) and Zahan et al (2011). The overall value of Dst (0.023) and the value of mean F st (0.034) were low, indicating a low genetic diversity between breeds.…”

Section: Discussioncontrasting

confidence: 73%

“…Many studies have been conducted in recent years on the genetic variability and diversity of the local sheep breeds, using microsatellites analysis. Some of these studies were done in the Balkans area, in Greece (Mastranestasis et al, 2015;Loukovitis et al, 2016), Turkey (Yilmaz et al, 2014), Bulgaria (Kusza et al, 2010) and Romania (Kevorkian et al, 2010). These studies reveal the rather precarious situation of these local breeds, which lose ground to the imported breeds, and the genetic diversity tends to decrease due to the shrinking numbers and because the stocks of sheep are reared increasingly isolated from one another.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity in main local sheep breeds from Romania using microsatellite markers

et al. 2020

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Abstract. The state of the local breeds of farm animals is increasingly precarious worldwide because of the aggressive introduction of breeds with improved economical traits. The preference of the breeders for local breeds is due to their higher adaptability to the particular climate and relief conditions of the mountain areas, to the high rate of assimilation of the feeds from these regions and to their increased resistance to diseases. This study analyzes the genetic variation of the main four local Romanian sheep breeds (Tsurcana, Tsigai, Ratska and Teleorman Blackhead) in terms of stock and economic importance, using 18 microsatellite markers. The mean number of alleles per locus was of 9.764. The values of genetic diversity parameters exhibited a high degree of polymorphism for the analyzed breeds, although inbreeding was highlighted particularly in Tsurcana and Tsigai. These breeds also showed an intense gene flow among them and were less differentiated in comparison with Ratska and Teleorman Blackhead. The results of this study may be useful for breeding programs and conservation plans since the genetic resources of the local breeds must be preserved so as to maintain an adequate level of biodiversity in animal husbandry.

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Section: Discussionsupporting

confidence: 84%

Section: Discussioncontrasting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity in main local sheep breeds from Romania using microsatellite markers

et al. 2020

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“…The global F IS observed in the present study is much lower than the previous reports in European sheep (0.123) [15], Nigerian sheep (0.335) [16], and Bulgarian sheep (0.288) [17]. In contrast, much lower F IS value of 0.024 has been reported in indigenous sheep breeds of China [18].…”

Section: Discussioncontrasting

confidence: 87%

Single-nucleotide polymorphism-based genetic diversity analysis of the Kilakarsal and Vembur sheep breeds

et al. 2017

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Aim:The present study was thus undertaken to analyze the genetic diversity of Kilakarsal and Vembur sheep breeds using single-nucleotide polymorphism (SNP) markers within Toll-like receptor (TLR) 3, 5, 6, 9, and 10 genes.Materials and Methods:Competitive allele-specific polymerase chain reaction (PCR)-based end-point genotyping was performed using real-time PCR to type the SNPs. Allele discrimination module implemented in real-time PCR was utilized to call the genotypes based on fluorescence intensity recorded for each of the two alleles. Basic diversity indices, namely, gene frequencies, observed heterozygosity, expected heterozygosity, and inbreeding coefficient (FIS), and testing for Hardy–Weinberg equilibrium (HWE) were estimated using package for elementary analysis of SNP data software program.Results:Of the 25 SNPs, 22 were found to be polymorphic, whereas two SNPs, namely, TLR3_1081_AC and TLR9_2036_CT, were monomorphic in both Kilakarsal and Vembur sheep populations. The SNP TLR10_1180_AG was monomorphic in Kilakarsal but polymorphic in Vembur sheep. The observed heterozygosities were estimated as 0.289 and 0.309 in Kilakarsal and Vembur sheep, respectively, whereas the expected heterozygosity values were 0.305 and 0.309 in the two breeds, respectively. The overall mean FIS was 0.107 ranging from −0.005 to 0.241 in Kilakarsal sheep and −0.047 ranging from −0.005 to 0.255 in Vembur sheep. In Kilakarsal sheep, the test for HWE revealed TLR9_1308_GC SNP locus with significant deviation (p<0.05) due to heterozygosity deficit. In Vembur sheep, TLR10_82_CT and TLR10_292_CG loci showed significant deviation (p<0.05) due to heterozygosity excess. Other SNP loci did not deviate from HWE (p>0.05) revealing that the population was in HWE proportions.Conclusions:The SNP markers within five TLR genes (TLR3, TLR5, TLR6, TLR9, and TLR10) utilized for genotyping in this study were highly polymorphic in Kilakarsal and Vembur breeds of sheep. This study on the genetic diversity analysis of the Kilakarsal and Vembur sheep breeds revealed considerable genetic variation within the breeds and it can be utilized to improve desirable traits.

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“…In the global and domestic practice of sheep rearing, microsatellite analysis is used to assess the genetic diversity of breeds and study phylogenetic relationships between them [6][7][8], proof of origin [9] and pedigree of animals [10], to investigate the population structure and genetic drift [11,12], and establish the level of inbreeding in specific groups and herds [13].…”

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confidence: 99%

Variability of Microsatellites in Sheep Breeds Raced in Russia

Deniskova¹,

Selionova²,

Гладырь³

et al. 2016

S-h. biol.

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A b s t r a c tAt the current stage of biological development is impossible to establish conservation programs and to monitor genetic resources of sheep without a preliminary study by DNA markers. The Russian sheep breeding is represented by wide variety of breeds, including all productivity and wool types. However, until recently only some sheep breeds, which belong to the same breeding zone or productivity type, were investigated by DNA markers including microsatellites. We studied 25 Russian sheep breeds (n = 751), including fine-fleeced -Dagestan Mountain (DAG), Grozny (GRZ), Kulunda (KUL), Manych Merino (MNM), Salskaya (SAL), Stavropol (STA), Soviet Merino (SVM), Volgograd (VOL), Baikal's fine-fleeced (ZBL); semi fine-fleeced -Altay Mountain (ALT), Kuibyshev (KUI), North Caucasian (NC), Russian long-haired (RLH), Tsigai (TSIG); coarsewooled -Andean (AND), Buubey (BUB), Edilbai (EDL), Karachaev (KAR), Kuchugur (KCH), Kalmyk (KLM), Karakul (KRK), Lezgin (LEZ), Romanov (ROM), Tushin (TSH), Tuvan short fat-tailed (TUV). The research was conducted using 11 microsatellite loci (OarCP49, INRA063, HSC, OarAE129, MAF214, OarFCB11, INRA005, SPS113, INRA23, MAF65 и McM527). The data were processed using GenAIEx 6.5 and PAST software. In general, the studied breeds were characterized by moderately high allelic diversity. The average number of alleles per locus is varied from 7.20±0.98 in KUL and 10.30±0.99 in TSIG. The values of Na≥10.0 were found in TSIG, TUV, BUB and KRK, values of Na≤8.0 were identified in KUL, RLH and SVM. The effective allele number was the highest in the KRK and TUV (Ne≥5.7) and the minimum was detected in KCH, ALT, RLH and NC (Ne≤4.3). The level of the observed heterozygosity in 21 of the 25 studied breeds ranged from 0.489±0.095 in TUV to 0.651±0.050 in ROM and 0.651±0.060 in SVM, and four other breeds (BUB, TSIG, ZBL and TUV) it varied from 0.798±0.023 in BUB up 0.977±0.017 in TUV. There was a substantial deficit of heterozygotes in 21 of the 25 studied breeds (F IS values ranged from 0.13 in ROM to 0.36 in KAR and SAL), in the other four (BUB, TSIG, ZBL and TUV) an excess of heterozygotes (F IS values ranged from 0.04 to 0.22) was detected. The analysis of molecular variance (AMOVA) showed that 5.02 % of genetic variation is composed of differences among breeds and 94.98 % is explained by within breeds' component. Analysis of the structure of the UMPGA phylogenetic tree, based on the matrix of pairwise genetic distances by M. Nei (1972), showed that the nature of the identified relationships is mainly related with the wool type, productivity type and breeding region. Thus, the identified polymorphism of eleven microsatellite loci is quite powerful for differentiating sheep of various breeds. For a better understanding population structure and obtaining new information on the genetic diversity at the genomic level the application of DNA microarrays, based on the multiple SNPs-markers, is required.

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Microsatellite analysis to estimate genetic relationships among five bulgarian sheep breeds

Cited by 17 publications

References 12 publications

Assessment of genetic diversity in main local sheep breeds from Romania using microsatellite markers

Assessment of genetic diversity in main local sheep breeds from Romania using microsatellite markers

Single-nucleotide polymorphism-based genetic diversity analysis of the Kilakarsal and Vembur sheep breeds

Variability of Microsatellites in Sheep Breeds Raced in Russia

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