Microsatellite analysis reveals genetic diversity of the endangered species<i>Dipterocarpus dyeri</i>

Nguyen, Thi Kim Oanh; Vu, Duy Dinh; Nguyen, Duc Minh; Dang, Hien Phan; Phan, Long Ke; Bui, Phuong Xuan

doi:10.1080/13416979.2020.1747149

Cited by 14 publications

(5 citation statements)

References 26 publications

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“…In view of the ecological and silvicultural importance of the Dipterocarpaceae, there have been many studies of dipterocarp trees that have investigated patterns of genetic diversity, fine-scale spatial genetic structure, mating system and gene flow among populations [18][19][20][21][22] The majority of studied species have moderate to high levels of genetic variation, and low differentiation among populations, suggesting a historical pattern of outcrossing combined with large, stable populations [3,8]. In addition, the genetic diversity and population structure of endangered species in the Dipterocarpaceae have been assessed for the purposes of conservation and management of genetic resources [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure in the endangered tree Hopea hainanensis (Dipterocarpaceae) on Hainan Island, China

Wang

Ren

et al. 2020

PLoS ONE

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Hopea hainanensis Merrill & Chun (Dipterocarpaceae) is an endangered tree species restricted to Hainan Island, China and a small part of Northern Vietnam. On Hainan Island, it is an important indicator species for tropical forests. However, because of its highly valued timber, H. hainanensis has suffered from overexploitation, leading to a sharp population decline. To facilitate the conservation of this species, genetic diversity and population structure were assessed using 12 SSR markers for 10 populations sampled across Hainan Island. Compared to non-threatened Hopea species, H. hainanensis exhibited reduced overall genetic diversity and increased population differentiation (AMOVA: FST = 0.23). Bayesian model-based clustering and principal coordinate analysis consistently assigned H. hainanensis individuals into three genetic groups, which were found to be widespread and overlapping geographically. A Mantel test found no correlation between genetic and geographical distances (r = 0.040, p = 0.418). The observed genetic structure suggests that long-distance gene flow occurred among H. hainanensis populations prior to habitat fragmentation. A recent population bottleneck was revealed, which may cause rapid loss of genetic diversity and increased differentiation across populations. Based on these findings, appropriate strategies for the long-term conservation of the endangered species H. hainanensis are proposed.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure in the endangered tree Hopea hainanensis (Dipterocarpaceae) on Hainan Island, China

Wang

Ren

et al. 2020

PLoS ONE

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“…Previous studies report that keruing species are under high threat due to illegal timber extraction and disturbed habitat (Rugayah et al 2017;Robiansyah 2017;Rachmat et al 2018). High exploitation without conservation effort (Denny and Susilo 2019;Nguyen et al 2020), specific habitat requirement (Purwaningsih 2004;Shivaprasad et al 2017), and long flowering period (Harrison et al 2005, Shivaprasad et al 2017) also accelerate the population decline of keruing in its natural habitat. There are 25 species of keruing in Indonesia that are listed in the International Union for Conservation of Nature (IUCN) red list with vulnerable status (1 species), endangered (1 species), and critically endangered (23 species).…”

Section: Introductionmentioning

confidence: 99%

Floristic composition and diversity at Keruing (Dipterocarpus spp.) habitat in Tangkahan, Gunung Leuser National Park, Indonesia

Susilowati

Rachmat²,

Elfiati

et al. 2021

Biodiversitas

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Abstract. Susilowati A, Rachmat HH, Elfiati D, Hidayat A, Hadi AN, Zaitunah A, Nainggolan D, Ginting IM. 2021. Floristic composition and diversity at Keruing (Dipterocarpus spp.) habitat in Tangkahan, Gunung Leuser National Park, Indonesia. Biodiversitas 22: 4448-4456. Dipterocarpus spp., or locally known as Keruing, is a wood-producing tree and has high economic value. Keruing also produces derivative products in oil and oleoresin, which are widely used as raw materials for medicines and perfumes. Keruing natural population has been severely declining caused by habitat alteration and also over exploitation. Therefore, information on the composition and diversity of the floristic community at their habitat is important as an initial step in designing conservation actions. This study aims to determine the floristic composition and diversity at the keruing habitat in the Tangkahan area, Gunung Leuser National Park (Gunung Leuser NP). A line transect has been used to establish a purposive sampling in particular forest areas where the population of the keruing is known to grow naturally. Four line transects, each with five nested plots, totaling 110 plots, were established to gather data at four growth stages. The results found 47 species at the seedling stage, 43 species at the sapling stage, 48 species at the pole stage and 65 species at the tree stage. There were three species of keruing in the research location, namely Dipterocarpus haseltii, Dipterocarpus costulatus and Dipterocarpus elongatus Korth with low IVI values ??at each stage. D. haseltii was only found at the seedling, pole and tree stage with IVI values ??of 0.32, 0.30, and 6.90, respectively. D. costulatus was only found at the tree level with an IVI value of 3.43, while D. elongatus found at the sapling and tree stage with an IVI of 0.30 and 3.42, respectively. The diversity index in the research location was in the medium category at the seedling, sapling, and pole levels with a value of 2.96, 2.86, and 2.92, respectively and was classified as high at the tree level with a value of 3.18. The evenness index (E') in all growth stages is high or evenly distributed with the value of E' > 0.75. The species diversity in research location classified into medium-high category although in each stage showing dynamic on species domination. Disturbed habitat due to illegal logging and land conversion caused a problem on keruing regeneration. Conservation efforts, including active monitoring keruing occurrence, ex-situ conservation, and artificial regeneration, are needed to avoid keruing from extinction.

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“…Currently, there are no published assessments about the impact of the exploitation of Ternstroemia fruits on its genetic diversity and demography. However, similar systems (trees under some exploitation) such as Aquilaria [ 21 ], Cedrus [ 22 ], and Dipterocarpus [ 23 ] have been genetically evaluated using microsatellites or simple sequence repeats (SSRs). This technique offers advantages such as codominance, high polymorphism, individual resolution, technical simplicity, and the ease of applying it to degraded DNA, such as herbarium vouchers [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic-based microsatellite development for Ternstroemia (Pentaphylacaceae) and transferability to other Ericales

et al. 2023

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Background The genus Ternstroemia is associated with the vulnerable tropical montane cloud forest in Mexico and with other relevant vegetation types worldwide. It contains threatened and pharmacologically important species and has taxonomic issues regarding its species limits. This study describes 38 microsatellite markers generated using a genomic-based approach. Methods and results We tested 23 of these markers in a natural population of Ternstroemia lineata. These markers are highly polymorphic (all loci polymorphic with 3–14 alleles per locus and expected heterozygosity between 0.202 and 0.908), most of them (19 out of 23) are in Hardy-Weinberg Equilibrium and free of null alleles (18 out of 23). Also we found no evidence of linkage among them. Finally, we tested the transferability to six other American species of Ternstroemia, two other Pentaphylacaceae species, and four species from different families within the order Ericales. Conclusions These molecular resources are promising tools to investigate genetic diversity loss and as barcodes for ethnopharmacological applications and species delimitation in the family Pentaphylacaceae and some Ericales, among other applications.

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Microsatellite analysis reveals genetic diversity of the endangered speciesDipterocarpus dyeri

Cited by 14 publications

References 26 publications

Genetic diversity and population structure in the endangered tree Hopea hainanensis (Dipterocarpaceae) on Hainan Island, China

Genetic diversity and population structure in the endangered tree Hopea hainanensis (Dipterocarpaceae) on Hainan Island, China

Floristic composition and diversity at Keruing (Dipterocarpus spp.) habitat in Tangkahan, Gunung Leuser National Park, Indonesia

Genomic-based microsatellite development for Ternstroemia (Pentaphylacaceae) and transferability to other Ericales

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