Microsatellite analysis of genetic population structure of the endangered cyprinid Anaecypris hispanica in Portugal: implications for conservation

Salgueiro, Patrícia; Carvalho, Gary R.; Collares‐Pereira, Maria João; Coelho, M. M.

doi:10.1016/s0006-3207(02)00132-5

Cited by 69 publications

(63 citation statements)

References 34 publications

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“…The use of microsatellite loci characterized from other Brycon species has proven to be suitable for microsatellite amplification of B. insignis. Similar conclusions were reached by Abila et al (2004) for cichlid fish, and Salgueiro et al (2003) for cyprinid fish. All five primer pairs used in the present study were polymorphic and amplified consistent and reproducible microsatellite alleles with the allele size ranging from 72 (BC48-10) to 194 bp (B0M13) ( Table 3).…”

Section: Efficiency Of Cross-amplification In Microsatellite Primerssupporting

confidence: 79%

Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management

Matsumoto

Hilsdorf

2009

Neotrop. ichthyol.

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Piabanha (Brycon insignis) is a freshwater fish species from the drainages in Southeastern Brazil. During the 1950s, it was an important economic and food resource for local populations, but dramatic and continuous environmental degradation seriously jeopardized the B. insignis populations in the region. Microsatellite markers were used to assess the genetic structure of wild populations of B. insignis and compare the genetic variability and integrity of the wild populations with a captive population. Samples of DNA from 208 specimens from geographically isolated populations were analyzed. Population genetic structure was investigated using F ST , R ST estimates as well as AMOVA. All five loci used in this study were polymorphic with observed heterozygosity ranging from 0.77 (± 0.15) to 0.88 (± 0.07) in the wild population and 0.90 (± 0.09) in the captive population and the allelic richness average were 7.56 (± 0.27) and 5.80 (± 1.02), respectively. Overall genetic differences were significantly partitioned among populations (F ST = 0.072, p = 0.034). Evidence of a genetic bottleneck was found in some of the wild populations, but especially in the captive population. The results showed that genetic variability still can be found in B. insignis populations which are currently structured possibly due to anthropic actions. The implications of these findings for the management and conservation of B. insignis populations are discussed.Piabanha (Brycon insignis) é uma espécie de peixe de água doce oriunda de drenagens da região sudeste do Brasil. Durante os anos de 1950, esta espécie foi um importante recurso econômico para populações locais. Contudo, a intensa e contínua degradação ambiental afetou seriamente as populações de B. insignis na região. Marcadores microssatélites foram usados para avaliar a estrutura genética de populações selvagens de B. insignis e comparar a variabilidade genética e integridade das populações selvagens com uma população de cativeiro. Amostras de DNA de 208 espécimes de populações geograficamente isoladas foram analisadas. A estrutura populacional foi investigada usando-se estimadores de F ST e R ST bem como AMOVA. Todos os loci usados neste estudo foram polimórficos com heterozigosidades observadas variando de 0.77 (± 0.15) a 0.88 (± 0.77) em populações selvagens e 0.90 (± 0.09) na população de cativeiro e a riqueza alélica média foi de 7.56 (± 0.27) e 5.80 (± 1.02), respectivamente. A maioria das diferenças genéticas foi significativa entre populações (F ST = 0.072, p = 0.034). Evidências de efeito gargalo foram observadas em algumas populações selvagens e especialmente também na população de cativeiro. Os resultados do presente estudo mostraram que as populações de B. insignis ainda apresentam variabilidade genética e que estas populações estão atualmente estruturadas geneticamente provavelmente devido a ações antrópicas. As implicações destes achados para o manejo e conservação das populações de B. insiginis são discutidas.

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Section: Efficiency Of Cross-amplification In Microsatellite Primerssupporting

confidence: 79%

Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management

Matsumoto

Hilsdorf

2009

Neotrop. ichthyol.

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“…Compared to other endangered freshwater fishes from Iberia or elsewhere, I. olisiponensis shows extremely low levels of genetic polymorphism and high population subdivision (e.g. Alves et al 2001a;Salgueiro et al 2003;Mesquita et al 2005;Sousa et al 2010;Dowling et al 2012;Lopes-Cunha et al 2012;Osborne et al 2012;Sterling et al 2012;Chen et al 2013). Furthermore, in addition to the suspected old hybridization events discussed above, we present clear evidence that I. olisiponensis is currently being subjected to strong genetic assimilation through hybridization with the sympatric I. lusitanicum (Gante et al 2010 and present study).…”

Section: Taxonomic Considerationssupporting

confidence: 66%

Evolutionary history and population genetics of a cyprinid fish (Iberochondrostoma olisiponensis) endangered by introgression from a more abundant relative

et al. 2014

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“…As mentioned, A. hispanica is an endemism of the Guadiana streams and is in threat of extinction (Collares-Pereira & Cowx 2001). The conservation status of A. hispanica changed drastically in few years, from abundant to threatened with extinction (Collares-Pereira et al 1999, Salgueiro et al 2003. Damming and water abstraction for irrigation explain this change, once it destroys or damages the habitat, interferes with reproduction opportunities, may generate polluted effluents and facilitate the introduction of non-native competitor species (Collares-Pereira et al 2000).…”

Section: Water Quantity and Fish Populationsmentioning

confidence: 99%

Review on the major ecosystem impacts caused by damming and watershed development in an Iberian basin (SW-Europe): focus on the Guadiana estuary

Morais

2008

Ann. Limnol. - Int. J. Lim.

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The Guadiana estuary (Iberian Peninsula, Europe) has low agriculture, industry and urban pressure, unlike other major Iberian estuaries; however, this situation is about to change. The Alqueva dam was constructed at 150 km from the river mouth, creating one of the biggest artificial lakes in Europe, which will support 110 000 ha of irrigation areas. Moreover, the estuarine margins are suffering increased human pressure. The risks that the Alqueva dam will pose to the downstream ecosystems, mainly to the estuary and adjacent coastal area, will be reviewed in this paper. Other issues are also analysed, as increased human desertification in the Portuguese basin, real estate pressure and the impact of the construction of a public infrastructure in the Portuguese lower estuary. Increased problems of sediment load reduction, water quality deterioration and shifts in the downstream fish populations are expected due to higher river flow reduction. Irreversible changes to estuarine margins and increased risk of pollution in the estuary will result from the multiple urban pressures. Lack of forest management and increased risk of wildfires, leading to water quality degradation and increased soil erosion, are a result of human desertification in the upper Portuguese basin. Multiple solutions to tackle these problems have to be considered. Creating the International Natural Park of the Lower Guadiana would not be enough and tourism should not be regarded as the only way to develop such a poor region. An ecohydrological approach of the basin should be implemented, to establish scientific based solutions to help solving conflicts and testing scenarios. Ultimately, the creation of a Portuguese-Spanish Basin Management Council for the lower Guadiana, involving those that are interested in the sustainable development of the Guadiana (local populations and associations, private stakeholders (dam managers, tourism entrepreneurs, farmers), local and national authorities, ENGO's and scientists) is essential to produce ecological and sustainable management decisions.

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Microsatellite analysis of genetic population structure of the endangered cyprinid Anaecypris hispanica in Portugal: implications for conservation

Cited by 69 publications

References 34 publications

Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management

Microsatellite variation and population genetic structure of a neotropical endangered Bryconinae species Brycon insignis Steindachner, 1877: implications for its conservation and sustainable management

Evolutionary history and population genetics of a cyprinid fish (Iberochondrostoma olisiponensis) endangered by introgression from a more abundant relative

Review on the major ecosystem impacts caused by damming and watershed development in an Iberian basin (SW-Europe): focus on the Guadiana estuary

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