Microsatellite analysis of genetic diversity and population structure of Jordanian barley (<i>Hordeum vulgare</i> L.) reveals genetic polymorphism and divergence associated with inflorescence type

Hasan, Maen; Odat, Nidal; Qrunfleh, Issam M.; Shakhatreh, Y.; Saifan, Sobhia

doi:10.5958/2348-7542.2018.00015.3

Cited by 6 publications

(2 citation statements)

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“…Therefore, for efficient utilization of barley landraces in breeding programs and to develop the strategies for their optimal conservation, it is necessary systematically to evaluate molecular diversity encompassed in the landraces collections 17 . Among the DNA markers, SSR markers have been extensively use to analyze population structure and genetic diversity in barley germplasm, due to the high polymorphism, reproducibility, co-dominant and multi-allelic nature when compared to most of the marker systems [16][17][18][19][20][21][22] .…”

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confidence: 99%

The influence of breeding history, origin and growth type on population structure of barley as revealed by SSR markers

Mohammadi

Sisi

Sadeghzadeh³

2020

Sci Rep

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Natural and mass selection during domestication and cultivation favored particular traits of interest in barley. In the present study, population structure, and genetic relationships among 144 accessions of barley landraces and breeding materials from various countries were studied using a set of 77 and 72 EST-SSR and gSSR markers, respectively distributed on seven chromosomes of barley. In total, 262 and 429 alleles were amplified in 77 EST-SSRs and 72 gSSR loci, respectively. Out of which, 185 private/group-specific alleles were identified in the landraces compared with 14 in "cultivar and advanced breeding lines", indicating the possibility to introgress favorite alleles from landraces into breeding materials. Comparative analysis of genetic variation among breeding materials, Iranian landraces, and exotic landraces revealed higher genetic diversity in Iranian landraces compared with others. A total of 37, 15, and 14 private/group-specific alleles were identified in Iranian landraces, exotic landraces, and breeding materials, respectively. The most likely groups for 144 barley genotypes were three as inferred using model- and distance-based clustering as well as principal coordinate analysis which assigned the landraces and breeding materials into separate groups. The distribution of alleles was found to be correlated with population structure, domestication history and eco-geographical factors. The high allelic richness in the studied set of barley genotype provides insights into the available diversity and allows the construction of core groups based on maximizing allelic diversity for use in barley breeding programs.

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confidence: 99%

The influence of breeding history, origin and growth type on population structure of barley as revealed by SSR markers

Mohammadi

Sisi

Sadeghzadeh³

2020

Sci Rep

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“…In the last years, new markers were used as a powerful methods for characterization and determination of genetic variance among cultivars which are independent of environmental effects (Liu et al, 2006 andVelicevici et al, 2012). The discovery of polymerase chain reaction (PCR) make revolution in genetic field and create numerous molecular techniques like RAPD-PCR, simple sequence repeats and inter simple sequence repeat (Matus et al, 2002;Rashal et al, 2004;An et al, 2009 andHasan et al, 2018). Nevertheless, molecular markers have been applied in linkage map construction, variety identification, detecting alien chromosome substitutions and genetic diversity in barley (Tahir 2014 andElakhdar et al, 2018).…”

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confidence: 99%

Genetic Diversity among some Egyptian Barley (Hordeum vulgare L.) Cultivars Based on Protein and RAPD Markers

El-Maaty

2019

Journal of Agricultural Chemistry and Biotechnology

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Genetic variability of seven Egyptian barley cultivars was analyzed utilizing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNA (RAPD) techniques. The results of SDS-PAGE produced 12 bands ranging from 135 to 11 KDa. Two polymorphic bands at molecular weight (MW) 13 and 12 KDs were detected with 16.6% polymorphism among barely cultivars. In the other hand, five RAPD primers were employed to assess the genetic diversity and relationships between the seven Egyptian barley cultivars. RAPD analysis exhibited 93 amplicons (86% polymorphism) with an average number of 18.6 amplicons per primer. Genetic similarity of RAPD and protein input ranged from 0.31 to 0.83. The dendrogram of combined data had clustered all the cultivars into two main clusters; the first one containing all barley cultivars except Giza 123 cultivar which formed a separate cluster indicating that the genetic background of this cultivar was distinct from all cultivars. The results obtained from RAPD and protein analysis exhibit different level of polymorphism. RAPD profile is more suitable technique than SDS-PAGE in assessing genetic variation among the seven barely cultivars. Moreover, the present results suggest that, the increasing number of primers and using more different markers could be more accurate to discriminate the genetic variance between barley cultivars. Subsequently, this could be useful to differentiate between barley cultivars in the breeding program.

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Climate Change and Cereal Production

Al-Tawaha¹,

Dar²,

Sultan³

et al. 2022

Climate Change and Agriculture

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Microsatellite analysis of genetic diversity and population structure of Jordanian barley (Hordeum vulgare L.) reveals genetic polymorphism and divergence associated with inflorescence type

Cited by 6 publications

References 0 publications

The influence of breeding history, origin and growth type on population structure of barley as revealed by SSR markers

The influence of breeding history, origin and growth type on population structure of barley as revealed by SSR markers

Genetic Diversity among some Egyptian Barley (Hordeum vulgare L.) Cultivars Based on Protein and RAPD Markers

Climate Change and Cereal Production

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