Microsatellite analysis of clonality and individual heterozygosity in natural populations of aspen Populus tremula L.: Identification of highly heterozygous clone

Политов, Д. В.; Belokon, M. M.; Belokon, Yu. S.; Polyakova, T. A.; Shatokhina, A. V.; Мудрик, Е. А.; Khanov, N. A.; Shestibratov, Konstantin A.

doi:10.1134/s1022795416060107

Cited by 3 publications

(7 citation statements)

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“…Comparison of the first 24 specimens, collected on the aspen border outline, has shown that they all belong to four multilocus genotypes. Two trees (Meshabash#2 and Meshabash#25) on the southern edge of the plot represented genotype “Meshabash#5” ; five trees in the north at the periphery were identified as separate genotype “Meshabash#13” and one more tree on the northern edge was also genetically different and named as “Meshabash#14” ; all other 16 trees possessed the same genotype that was identical to the previously described as “Meshabash” clone [ 7 ]. Additional collection of 24 trees inside the contour formed by outermost SSR-identified ramets was done.…”

Section: Resultsmentioning

confidence: 99%

“…In the analysis of genetic relatedness between genotypes we used genotypic data for three native stands (“Prisady” in Moscow region, “Voronezh” in Voronezh region, and “Yoshkar-Ola” in Republic of Mari-El) taken from Politov et al [ 7 , 8 ].…”

Section: Methodsmentioning

confidence: 99%

“…The previously selected set of 14 microsatellite loci ORPM193 , ORPM202 , ORPM206 , ORPM220 , ORPM296 [ 11 ], WPMS14 , WPMS15 , WPMS16 , WPMS17 , WPMS18 , WPMS19 , WPMS20 , WPMS21 , and WPMS22 [ 12 ] was employed for DNA identification [ 7 , 8 ] of “ Meshabash ” clone ramets from putatively growing together in the same plot other aspen genotypes/clones.…”

Section: Methodsmentioning

confidence: 99%

“…Reagents used for PCRs, laboratory equipment, regimes of DNA amplification, fragment analysis, and gel documentation were performed as described earlier [ 7 , 8 ]. PCR products were analyzed by electrophoresis in vertical blocks of 6% polyacrylamide gel in the tris-EDTA-borate buffer system.…”

Section: Methodsmentioning

confidence: 99%

“…In this paper, we report the results of karyological analysis of a rampant quaking aspen clone “Meshabash” that we previously revealed in Sabinsky Experimental Forestry in the Republic of Tatarstan, Russia [ 7 ], and outline territory occupied by its ramets identified by DNA markers. We also present first pilot data on the growth rate and damage caused by aspen trunk rot in representative ramets of this clone.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Identification and Karyological Analysis of a Rampant Aspen Populus tremula L. (Salicaceae) Clone

Политов

Belokon

Shatokhina

et al. 2017

International Journal of Plant Genomics

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A rampant highly heterozygous aspen (Populus tremula L.) clone “Meshabash” has been revealed in course of population genetic diversity analysis in a native stand in the Republic of Tatarstan, Russia. Here we report the results of karyological analysis showing that this highly vigorous clone is diploid (2n = 38) while typically triploid aspen demonstrates increased growth rate and resistance to aspen trunk rot caused by fungus Phellinus tremulae. By means of DNA identification of a series of model trees using 14 SSR loci we outlined the area occupied by this clone (at least 1.94 ha) and demonstrated that its ramets constitute 40 out of 48 genotyped trunks on the plot with the maximal distance between ramets 254 m. Since aspen is able to regenerate after cutting or die-off of maternal tree by root suckers at a distance up to 20–35 m this assumed that current stand appeared as a result of such spreading from an ortet tree during at least 5 generations. Trunk rot damage in the wood of model trees indicated low influence of this pathogen on viability and performance of the studied clone that can be associated with its extreme heterozygosity level (0.926) exceeding all the studied trees in this research plot and in three other control samples.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Identification and Karyological Analysis of a Rampant Aspen Populus tremula L. (Salicaceae) Clone

Политов

Belokon

Shatokhina

et al. 2017

International Journal of Plant Genomics

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A global view of aspen: Conservation science for widespread keystone systems

Rogers

Pinno

Šebesta

et al. 2020

Global Ecology and Conservation

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Genetic Uniqueness and Genetic Structure of Populations of Picea obovata Ledeb. and Larix sibirica Ledeb. in the Northern and Middle Urals

Zhulanov,

Chertov,

Nechaeva

et al. 2023

Forests

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Establishing sustainable plantations with genetic diversity equivalent to that of natural populations is vital for successful reforestation efforts. In this study, we present an innovative approach for selecting populations suitable for reforestation, taking into account their genetic uniqueness using Inter Simple Sequence Repeats (ISSR) markers. Our investigation focused on six populations of Picea obovata Ledeb and six populations of Larix sibirica Ledeb, collected from the Northern and Middle Urals. We found that the indicators of genetic diversity were significantly higher in L. sibirica compared to P. obovata, while the number of rare alleles was greater in Siberian spruce (R = 19). Among the P. obovata populations, the Cherdyn’s forestry exhibited notably high genetic diversity, and for L. sibirica, the Gainy’s forestry stood out in this regard. Moreover, the genetic subdivision of the six P. obovata populations (GST = 0.331) was higher than that of the six L. sibirica populations (GST = 0.177). To ensure optimal seed selection considering the genetic originality coefficient (GOC) and population differentiation, we recommend utilizing the P. obovata population from Gainy’s forestry with a GOC of 0.554 and the L. sibirica population from Cherdyn’s forestry with a GOC of 0.372. These populations harbor typical alleles characteristic of the research region, making them ideal candidates for seed selection. Furthermore, the specific alleles identified can serve as valuable markers for determining the geographic origin of P. obovata and L. sibirica wood, aiding in efforts to trace the sources of these species in forestry and trade practices.

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Microsatellite analysis of clonality and individual heterozygosity in natural populations of aspen Populus tremula L.: Identification of highly heterozygous clone

Cited by 3 publications

References 10 publications

Molecular Identification and Karyological Analysis of a Rampant Aspen Populus tremula L. (Salicaceae) Clone

Molecular Identification and Karyological Analysis of a Rampant Aspen Populus tremula L. (Salicaceae) Clone

A global view of aspen: Conservation science for widespread keystone systems

Genetic Uniqueness and Genetic Structure of Populations of Picea obovata Ledeb. and Larix sibirica Ledeb. in the Northern and Middle Urals

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