Microsatellite-Aided Screening for Fertility Restoration Genes (Rf) Facilitates Hybrid Improvement

El-Namaky, Raafat; Sedeek, S. E. M.; Moukoumbi, Yonnelle Dea; Ortíz, Rodomiro; Manneh, Baboucarr

doi:10.1016/j.rsci.2016.04.003

Cited by 36 publications

(9 citation statements)

References 10 publications

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“…Some studies have reported that the two fertility restorer genes are additive in their inheritance and the effect of Rf4 appeared to be larger than that of Rf3 (Yao et al 1997;Sattari et al 2008;Cai et al 2013). Similar findings in respect of a major locus Rf4 for fertility restoration of the WA-CMS on the long arm of rice chromosome 10 have been reported earlier by several researchers (Zhang et al 2002;Ahmadikhah and Karlov 2006;Sattari et al 2007;Sheeba et al 2009;Revathi et al 2013;Cai and Zhang 2013;Kiani 2015;El-Namaky et al 2016). Interestingly, all fully fertile F 1 s showed presence of Rf4 gene specific amplification, which indicated that the reported markers are closely linked to the fertility restoration gene.…”

Section: Screening Of Restoration Abilitysupporting

confidence: 69%

Screening and validation of fertility restoration genes (Rf) in wild abortive CMS system of rice (Oryza sativa L.) using microsatellite markers

Bhati

Singh

Kumar

2018

Ind. Jrnl. Gen. Plnt. Bree.

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The present study was carried out with the objective of validating linked SSRs markers to Rf genes in diverse rice breeding lines. SSR markers enabled to determine the chromosomal locations of the two Rf genes (Rf3 and Rf4) in the wild-abortive cytoplasmic male sterility (WA-CMS) system. Forty breeding lines with no prior information on fertility restoration were screened with the help of molecular markers linked to major fertility genes Rf3 and Rf4. The breeding lines were crossed with two WA-CMS lines (IR79156A and Pusa 6A) and the 80 F 1 s were evaluated for pollen and spikelet fertility to identify restorers and maintainers. Four SSRs reported to be linked to Rf genes (Rf3 and Rf4) were used to detect the allelic status in 40 breeding lines. The results revealed that out of 40, eight lines found to be common effective restorers for both the CMS lines (IR 79156A and Pusa6A). Two markers (RM171 and RM6100) proved to be associated with Rf4 genes. Results demonstrated that these markers could be used to screen large number of restorers and non-restorer lines in hybrid rice breeding programs in short time.

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Section: Screening Of Restoration Abilitysupporting

confidence: 69%

Screening and validation of fertility restoration genes (Rf) in wild abortive CMS system of rice (Oryza sativa L.) using microsatellite markers

Bhati

Singh

Kumar

2018

Ind. Jrnl. Gen. Plnt. Bree.

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“…Moreover, multi-environment testing will be still necessary to assess whether their host plant resistance will remain across space and time. Microsatellite-aided screening for fertility restoration genes Rf may further facilitate hybrid development with high adaptability in West Africa [34]. Preliminary results are promising: some adapted breeding lines and cultivars had high grain yield per plant with cytoplasmic male sterility lines and may be used as fertility restorers.…”

Section: Discussionmentioning

confidence: 99%

Putting Plant Genetic Diversity and Variability at Work for Breeding: Hybrid Rice Suitability in West Africa

El-Namaky¹,

Coulibaly

Alhassan

et al. 2017

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Rice is a staple food in West Africa, where its demand keeps increasing due to population growth. Hence, there is an urgent need to identify high yielding rice cultivars that fulfill this demand locally. Rice hybrids are already known to significantly increase productivity. This study evaluated the potential of Asian hybrids with good adaptability to irrigated and rainfed lowland rice areas in Mali, Nigeria, and Senegal. There were 169 hybrids from China included in trials at target sites during 2009 and 2010. The genotype × environment interaction was highly significant (p < 0.0001) for grain yield indicating that the hybrids' and their respective cultivar checks' performance differed across locations. Two hybrids had the highest grain yield during 2010 in Mali, while in Nigeria, four hybrids in 2009 and one hybrid in 2010 had higher grain yield and matured earlier than the best local cultivar. The milling recovery, grain shape and cooking features of most hybrids had the quality preferred by West African consumers. Most of the hybrids were, however, susceptible to African rice gall midge (AfRGM) and Rice Yellow Mottle Virus (RMYV) isolate Ng40. About 60% of these hybrids were resistant to blast. Hybrids need to incorporate host plant resistant for AfRGM and RYMV to be grown in West Africa.

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“…Nuclear Rf genes complement the male-sterile phenotype that arises with CMS, allowing the use of the CMS system for hybrid seed production. Molecular mapping of the Rf genes provides a powerful tool to develop restorer lines avoiding extensive testcrossing with CMS lines [41]. In this study, QTL analysis for fertility restoration of the DA-, DWR-, and ID-type CMS systems was conducted using two O .…”

Section: Discussionmentioning

confidence: 99%

Mapping QTLs for Fertility Restoration of Different Cytoplasmic Male Sterility Types in Rice Using Two Oryza sativa ×O. rufipogon Backcross Inbred Line Populations

Xie

Wan

et al. 2016

BioMed Research International

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Hybrid rice breeding using cytoplasmic male sterility/fertility restoration (CMS/Rf) systems plays an important role in ensuring global food security. Two backcross inbred line (BIL) populations derived from either Xieqingzao B (XB)//XB/Dongxiang wild rice (DWR) (XXD) or XB//DWR/XB (XDX) were used to detect quantitative trait loci (QTLs) for fertility restoration of Dwarf wild abortive- (DA-), Indonesia Paddy- (ID-), and DWR-type CMS in rice. Lines with ID- and DA-type CMS were testcrossed with both the XXD- and XDX-BILs, while the line with DWR-type CMS was testcrossed with the XDX-BILs only. A total of 16 QTLs for fertility restoration of CMS systems were identified, including three for DWR-type CMS, six for DA-type CMS, and seven for ID-type CMS. All of the additive alleles in the QTLs were derived from Oryza rufipogon. Eleven QTLs were clustered in five chromosomal regions, indicating that common Rf loci restored different CMS systems, and the favorable O. rufipogon alleles could be used to develop restorer lines for various CMS types by marker-assisted selection.

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Microsatellite-Aided Screening for Fertility Restoration Genes (Rf) Facilitates Hybrid Improvement

Cited by 36 publications

References 10 publications

Screening and validation of fertility restoration genes (Rf) in wild abortive CMS system of rice (Oryza sativa L.) using microsatellite markers

Screening and validation of fertility restoration genes (Rf) in wild abortive CMS system of rice (Oryza sativa L.) using microsatellite markers

Putting Plant Genetic Diversity and Variability at Work for Breeding: Hybrid Rice Suitability in West Africa

Mapping QTLs for Fertility Restoration of Different Cytoplasmic Male Sterility Types in Rice Using Two Oryza sativa ×O. rufipogon Backcross Inbred Line Populations

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