MicroRNAs associated with molecular mechanisms for plant root formation and growth

Tang, Wei; Tang, Ao

doi:10.1007/s11676-015-0183-0

Cited by 9 publications

(6 citation statements)

References 77 publications

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“…Although enhanced salt tolerance via overexpression of TFs has been documented in many different plant species [ 66 , 67 , 71 , 84 ], molecular mechanisms of transcription factor-enhanced salt stress tolerance are not fully understood [ 70 , 72 , 73 ]. Overexpression of ZAT6 enhanced salt stress tolerance is not reported in cells of P. elliottii , O. sativa , and G. hirsutum .…”

Section: Resultsmentioning

confidence: 99%

Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

Tang

Luo

2018

Open Life Sciences

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The purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of Arabidopsis thaliana 6 (ZAT6) in salt stress tolerance in cells of rice (Oryza sativa L.), cotton (Gossypium hirsutum L.) and slash pine (Pinus elliottii Engelm.). Cells of O. sativa, G. hirsutum, and P. elliottii overexpressing ZAT6 were generated using Agrobacterium-mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of ZAT6 increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA8, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, ZAT6 also increased expression of Ca2+-dependent protein kinase genes OsCPK9 and OsCPK25 by 5–7 fold under NaCl stress. Altogether, our results suggest that overexpression of ZAT6 enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca2+-dependent protein kinase in transgenic cell lines of different plant species.

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Section: Resultsmentioning

confidence: 99%

Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

Tang

Luo

2018

Open Life Sciences

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“…miRNAs, such as miR160 and miR393, may participate in the disease resistance of plants 18 . Our results suggest that miR2118 might play a role in disease resistance in P. notoginseng through its regulation of the NB-LRR genes, in addition to miR160 and miR393.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, by targeting three SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, miR156 plays a role in lateral root development in Arabidopsis 15 . Thus, miRNAs play critical roles in growth and development of plant roots 16 – 18 . It is interesting to note that miR156 overexpression could enhance shoot biomass accumulation as shown recently in switchgrass, a bioenergy plant species 19 .…”

Section: Introductionmentioning

confidence: 99%

Small RNA profiles from Panax notoginseng roots differing in sizes reveal correlation between miR156 abundances and root biomass levels

Zheng

Chen

et al. 2017

Sci Rep

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Plant genomes encode several classes of small regulatory RNAs (sRNAs) that play critical roles in both development and stress responses. Panax notoginseng (Burk.) F.H. Chen (P. notoginseng) is an important traditional Chinese herbal medicinal plant species for its haemostatic effects. Therefore, the root yield of P. notoginseng is a major economically important trait since the roots of P. notoginseng are the parts used to produce medicine. To identify sRNAs that are critical for the root biomass of P. notoginseng, we performed a comprehensive study of miRNA transcriptomes from P. notoginseng roots of different biomasses. We identified 675 conserved miRNAs, of which 180 pre-miRNAs are also identified, and three TAS3 loci in P. notoginseng. By using degradome sequencing, we identified 79 conserved miRNA:target or tasiRNA:target interactions, of which eight were further confirmed with the RLM 5′-RACE experiments. More importantly, our results revealed that a member of miR156 family and one of its SPL target genes have inverse expression levels, which is tightly correlated with greater root biomass contents. These results not only contributes to overall understanding of post-transcriptional gene regulation in roots of P. notoginseng but also could serve as markers for breeding P. notoginseng with greater root yield.

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“…Root growth and development directly affect plant uptake of minerals and water [ 12 ]. While plant roots exhibit high phenotypic plasticity [ 13 ], their growth and development are regulated by multiple factors. It has been found that miRNAs and TFs can form miRNA-TFs regulatory modules to regulate plant root development in a stepwise manner.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of the longan R2R3-MYB gene family and its role in primary and lateral root

Lv,

Tian,

Huang

et al. 2023

BMC Plant Biol

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R2R3-MYB is an important transcription factor family that regulates plant growth and development. Root development directly affects the absorption of water and nutrients by plants. Therefore, to understand the regulatory role of R2R3-MYB transcription factor family in root development of longan, this study identified the R2R3-MYB gene family members at the genome-wide level, and analyzed their phylogenetic characteristics, physical and chemical properties, gene structure, chromosome location and tissue expression. The analysis identified 124 R2R3-MYB family members in the longan genome. Phylogenetic analysis divided these members into 22 subfamilies, and the members of the unified subfamily had similar motifs and gene structures. The result of qRT-PCR showed that expression levels of DlMYB33, DlMYB34, DlMYB59, and DlMYB77 were significantly higher in main roots than in lateral as opposed to those of DlMYB35, DlMYB69, DlMYB70, and DlMYB83, which were significantly lower. SapBase database prediction and miRNAs sequencing results showed that 34 longan miRNAs could cleave R2R3-MYB, including 17 novel miRNAs unique to longan. The qRT-PCR and subcellular localization experiments of DlMYB92 and DlMYB98 showed that DlMYB92 is a key factor that regulates transcription in the nucleus and participates in the regulation of longan lateral root development. Longan also has a conserved miRNA-MYB-lateral root development regulation mechanism. This study provides a reference for further research on the transcriptional regulation of the miRNA-R2R3-MYB module in the root development of longan.

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MicroRNAs associated with molecular mechanisms for plant root formation and growth

Cited by 9 publications

References 77 publications

Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

Small RNA profiles from Panax notoginseng roots differing in sizes reveal correlation between miR156 abundances and root biomass levels

Genome-wide identification of the longan R2R3-MYB gene family and its role in primary and lateral root

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