Genome-wide identification of the longan R2R3-MYB gene family and its role in primary and lateral root

Abstract

R2R3-MYB is an important transcription factor family that regulates plant growth and development. Root development directly affects the absorption of water and nutrients by plants. Therefore, to understand the regulatory role of R2R3-MYB transcription factor family in root development of longan, this study identified the R2R3-MYB gene family members at the genome-wide level, and analyzed their phylogenetic characteristics, physical and chemical properties, gene structure, chromosome location and tissue expression. The analysis identified 124 R2R3-MYB family members in the longan genome. Phylogenetic analysis divided these members into 22 subfamilies, and the members of the unified subfamily had similar motifs and gene structures. The result of qRT-PCR showed that expression levels of DlMYB33, DlMYB34, DlMYB59, and DlMYB77 were significantly higher in main roots than in lateral as opposed to those of DlMYB35, DlMYB69, DlMYB70, and DlMYB83, which were significantly lower. SapBase database prediction and miRNAs sequencing results showed that 34 longan miRNAs could cleave R2R3-MYB, including 17 novel miRNAs unique to longan. The qRT-PCR and subcellular localization experiments of DlMYB92 and DlMYB98 showed that DlMYB92 is a key factor that regulates transcription in the nucleus and participates in the regulation of longan lateral root development. Longan also has a conserved miRNA-MYB-lateral root development regulation mechanism. This study provides a reference for further research on the transcriptional regulation of the miRNA-R2R3-MYB module in the root development of longan.

Genome-wide identification, classification and expression analysis of MYB gene family in coconut (Cocos nucifera L.)

“…In a separate investigation involving 124 longan R2R3-MYB family members, their protein sequences ranged from 134 to 661 amino acids, with an average length of 307 amino acids. The molecular masses ranged from 15.69 to 775.81 kDa, with an average of 34.68 kDa and theoretical isoelectric points ranged from 4.91 to 10.6 ( Lv et al., 2023 ). Based on the findings of a previous study conducted by Zhang et al.…”

The MYB Transcription Factor Family in Eucommia ulmoides: Genome-Wide Identification, Characterization, and Network Analysis in Relation to the Rubber Biosynthetic Genes

“…The results showed that it had 510 pairs positively correlated between 45 EUG biosynthesis genes and 66 EuMYB genes (Figures 8 and 9). Among them, EuMYB112 had the highest degree of connection, which was positively correlated with 20 EUG biosynthesis genes, followed by EuMYB12 (19), EuMYB51 (18), EuMYB60 (18), EuMYB77 (18), EuMYB82 (18), and EuMYB103 (18). At the same time, it had 198 pairs negatively correlated between 41 EUG biosynthesis genes and 57 EuMYB genes.…”

“…EuMYB12 (19), EuMYB51 (18), EuMYB60 (18), EuMYB77 (18), EuMYB82 (18), and EuMYB103 (18). At the same time, it had 198 pairs negatively correlated between 41 EUG biosynthesis genes and 57 EuMYB genes.…”

“…We predicted that these EuMYBs may have a possible regulatory relationship with the FPS1 structural gene. EuMYB12 (19), EuMYB51 (18), EuMYB60 (18), EuMYB77 (18), EuMYB82 (18), and EuMYB103 (18). At the same time, it had 198 pairs negatively correlated between 41 EUG biosynthesis genes and 57 EuMYB genes.…”

“…They are also involved in the expression of genes related to biosynthesis, such as anthocyanins and flavonoids [15]. Based on the findings above, the MYB TF family is widely involved in plants' biological development processes, hormone signaling, and primary and secondary metabolism [16][17][18][19].…”