MicroRNA319a regulates plant resistance to <i>Sclerotinia</i> stem rot

Dong, Weiguo; Ren, Wenqing; Wang, Xuan; Mao, Yanfei; He, Yuqing

doi:10.1093/jxb/erab070

Cited by 13 publications

(7 citation statements)

References 46 publications

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“…In Brassica rapa plants, overexpression of BraTCP4-1 promoted plant resistance to infection with Sclerotinia sclerotiorum [ 29 ]. However, overexpression of BraMIR319a , with BraTCP4-1 as its target gene, increased the susceptibility of the plants to S. sclerotiorum infection and then reduced the resistance to stem rot disease in B. rapa [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

PeCIN8 expression correlates with flower size and resistance to yellow leaf disease in Phalaenopsis orchids

Wu,

Chen,

Hsu

et al. 2023

BMC Plant Biol

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Background The orchid industry has seen a recent surge in export values due to the floral morphology and versatile applications of orchids in various markets for medicinal, food additive, and cosmetic usages. However, plant-related diseases, including the yellow leaf disease caused by Fusarium solani, have caused significant losses in the production value of Phalaenopsis (up to 30%). Results In this study, 203 Phalaenopsis cultivars were collected from 10 local orchid nurseries, and their disease severity index and correlation with flower size were evaluated. Larger flowers had weaker resistance to yellow leaf disease, and smaller flowers had stronger resistance. For the genetic relationship of disease resistance to flower size, the genetic background of all cultivars was assessed using OrchidWiz Orchid Database Software and principal component analysis. In addition, we identified the orthologous genes of BraTCP4, namely PeIN6, PeCIN7, and PeCIN8, which are involved in resistance to pathogens, and analyzed their gene expression. The expression of PeCIN8 was significantly higher in the most resistant cultivars (A7403, A11294, and A2945) relative to the most susceptible cultivars (A10670, A6390, and A10746). Conclusions We identified a correlation between flower size and resistance to yellow leaf disease in Phalaenopsis orchids. The expression of PeCIN8 may regulate the two traits in the disease-resistant cultivars. These findings can be applied to Phalaenopsis breeding programs to develop resistant cultivars against yellow leaf disease.

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Section: Discussionmentioning

confidence: 99%

PeCIN8 expression correlates with flower size and resistance to yellow leaf disease in Phalaenopsis orchids

Wu,

Chen,

Hsu

et al. 2023

BMC Plant Biol

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“…lobophylla . In Arabidopsis , AtTCP2 is one of the target genes of miRNA319 [ 15 ]. The significantly upregulated expression of miRNA319 resulted in the inhibition of the expression of the AtTCP2 transcription factor and the lobes of the leaf margin [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…From the perspective of plant evolution and phylogeny, the formation of plant leaf morphology is mainly determined by genes, miRNAs, and other regulatory factors. A number of miRNAs and their target genes have been shown to determine leaf development, such as miRNA156- SPL ( SQUAMOSA PROMOTER BINDING PROTEIN-LIKE ) [ 13 ], miRNA164- CUC ( CUP-SHAPED COTYLEDON ) [ 14 ], and miRNA319- TCP ( TEOSINTE BRANCHED 1/CYCLOIDEA/PCF ) [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla

Sun

Long

et al. 2022

IJMS

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A lobed leaf is a common trait in plants, but it is very rare in Lauraceae plants, including species of Phoebe. In the study of germplasm resources of Phoebe neurantha, we found lobed leaf variant seedlings, and the variation could be inherited stably. Studying the lobed leaf mechanism of P. neurantha var. lobophylla can offer insight into the leaf development mechanism of woody plants. RNA-seq and small RNA-seq analysis results showed that a total of 8091 differentially expressed genes (DEGs) and 16 differentially expressed miRNAs were identified in P. neurantha var. lobophylla. Considering previous research results, a leaf margin morphological development related miRNA, pne-miRNA319a, was primary identified as a candidate miRNA. Target gene prediction showed that a total of 2070 genes were predicted to be the target genes of differentially expressed miRNAs. GO enrichment analysis of differentially expressed target genes suggested that PnTCP2 is related to lobed leaf formation. The TRV-VIGS gene silencing of PnTCP2 led to lobed leaves in P. neurantha seedlings. The downregulation of PnTCP2 led to lobed leaves. The yeast two-hybrid test and bimolecular fluorescence complementation test confirmed that the PnTCP2 protein interacted with the PnLBD41 protein. Based on the expression analysis of gene-silenced leaves and RNA-seq and small RNA-seq analysis results, pne- miRNA319a and PnLBD41 might also play important roles in this process. In conclusion, PnTCP2 plays an important and vital role in the formation of the lobed leaves of P. neurantha var. lobophylla.

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“…In parallel, the miR160 target gene StARF16 (a gene involved in growth and development) regulates StNPR1 gene expression and thereby inhibits the SA pathway (Kalsi et al, 2022). In B. rapa, miR319a is involved in regulating plant resistance to stem rot disease caused by Sclerotinia sclerotiorum, and overexpression of MIR319A reduced plant resistance to SSR due to interruption of the JA-and SA-related pathways (Dong et al, 2021). AP2 belongs to the AP2/EREBP family of transcription factors and plays an important role in pathogen resistance (Cheng et al, 2016).…”

Section: Micrornas Are Involved In Plant Hormone Signalingmentioning

confidence: 99%

The roles of microRNAs in horticultural plant disease resistance

Zhang

Zhang²,

Wang³

et al. 2023

Front. Genet.

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The development of the horticultural industry is largely limited by disease and excessive pesticide application. MicroRNAs constitute a major portion of the transcriptomes of eukaryotes. Various microRNAs have been recognized as important regulators of the expression of genes involved in essential biological processes throughout the whole life cycle of plants. Recently, small RNA sequencing has been applied to study gene regulation in horticultural plants. In this review, we summarize the current understanding of the biogenesis and contributions of microRNAs in horticultural plant disease resistance. These microRNAs may potentially be used as genetic resources for improving disease resistance and for molecular breeding. The challenges in understanding horticultural plant microRNA biology and the possibilities to make better use of these horticultural plant gene resources in the future are discussed in this review.

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MicroRNA319a regulates plant resistance to Sclerotinia stem rot

Cited by 13 publications

References 46 publications

PeCIN8 expression correlates with flower size and resistance to yellow leaf disease in Phalaenopsis orchids

PeCIN8 expression correlates with flower size and resistance to yellow leaf disease in Phalaenopsis orchids

The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla

The roles of microRNAs in horticultural plant disease resistance

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