The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla

Sun, Bing; He, Xinru; Long, Fengying; Yu, Changlong; Fei, Yongjun

doi:10.3390/ijms232113296

Cited by 2 publications

(1 citation statement)

References 52 publications

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“…miRNAs are fundamental regulators of plant growth and development. A subset of CIN -like genes in many plants analyzed to date are targets of the miR319 and miR319-TCPs regulatory systems, which are widely involved in the development of plant roots ( Baulies et al., 2022 ), leaves ( Koyama et al., 2017 ; Sun et al., 2022 ), flowers ( Nag et al., 2009 ; Silva et al., 2019 ), and plant immunity ( Lopez et al., 2015 ). Interestingly, we found that miR159 shares the same target genes as miR319.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of TCP gene family in Dendrobium nobile and their role in perianth development

Wei,

Yuan,

Zheng

et al. 2024

Front. Plant Sci.

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TCP is a widely distributed, essential plant transcription factor that regulates plant growth and development. An in-depth study of TCP genes in Dendrobium nobile, a crucial parent in genetic breeding and an excellent model material to explore perianth development in Dendrobium, has not been conducted. We identified 23 DnTCP genes unevenly distributed across 19 chromosomes and classified them as Class I PCF (12 members), Class II: CIN (10 members), and CYC/TB1 (1 member) based on the conserved domain and phylogenetic analysis. Most DnTCPs in the same subclade had similar gene and motif structures. Segmental duplication was the predominant duplication event for TCP genes, and no tandem duplication was observed. Seven genes in the CIN subclade had potential miR319 and -159 target sites. Cis-acting element analysis showed that most DnTCP genes contained many developmental stress-, light-, and phytohormone-responsive elements in their promoter regions. Distinct expression patterns were observed among the 23 DnTCP genes, suggesting that these genes have diverse regulatory roles at different stages of perianth development or in different organs. For instance, DnTCP4 and DnTCP18 play a role in early perianth development, and DnTCP5 and DnTCP10 are significantly expressed during late perianth development. DnTCP17, 20, 21, and 22 are the most likely to be involved in perianth and leaf development. DnTCP11 was significantly expressed in the gynandrium. Specially, MADS-specific binding sites were present in most DnTCP genes putative promoters, and two Class I DnTCPs were in the nucleus and interacted with each other or with the MADS-box. The interactions between TCP and the MADS-box have been described for the first time in orchids, which broadens our understanding of the regulatory network of TCP involved in perianth development in orchids.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of TCP gene family in Dendrobium nobile and their role in perianth development

Wei,

Yuan,

Zheng

et al. 2024

Front. Plant Sci.

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Promoter variations in a homeobox gene, BrLMI1, contribute to leaf lobe formation in Brassica rapa ssp. chinensis Makino

et al. 2023

Theor Appl Genet

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Leaves are the main consumed organ in leafy non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis Makino), and the shape of the leaves is an important economic trait. However, the molecular regulatory mechanism underlying lobed-leaf trait in non-heading Chinese cabbage remains unclear. Here, we identi ed a stable incompletely dominant major locus qLLA10 for lobed leaves formation in nonheading Chinese cabbage. Based on map-based cloning strategies, BrLMI1, a LATE MERISTEM IDENTITY1 (LMI1)-like gene, was predicted as the candidate gene for qLLA10. Genotyping analysis showed that promoter variations of BrLMI1 in two parents are responsible for elevating the expression in the lobed-leaf parent and ultimately causing the alternation in leaf shape between the two parents, and the promoter activity of BrLMI1 was signi cantly affected by the promoter variations. BrLMI1 was exclusively localized in the nucleus and expressed mainly at the tips of each lobe. Leaf lobe development was perturbed in BrLMI1-silenced plants produced by virus-induced gene silencing assays, and ectopic overexpression of BrLMI1 in Arabidopsis led to deeply lobed leaves never seen in the wild type, which indicates that BrLMI1 is required for leaf lobe formation in non-heading Chinese cabbage. These ndings suggested that BrLMI1 is a positive regulatory factor of leaf lobe formation in non-heading Chinese cabbage and that cis-regulatory variations lead to the phenotype of lobed or entire leaf margins, thus providing the theoretical basis for unraveling the molecular mechanism underlying lobed leaves phenotype in Brassica crops. Key messageBrLMI1 is a positive regulatory factor of leaf lobe formation in non-heading Chinese cabbage, and cisregulatory variations lead to the phenotype of lobed or entire leaf margins

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The Role of PnTCP2 in the Lobed Leaf Formation of Phoebe neurantha var. lobophylla

Cited by 2 publications

References 52 publications

Genome-wide identification and characterization of TCP gene family in Dendrobium nobile and their role in perianth development

Genome-wide identification and characterization of TCP gene family in Dendrobium nobile and their role in perianth development

Promoter variations in a homeobox gene, BrLMI1, contribute to leaf lobe formation in Brassica rapa ssp. chinensis Makino

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