MicroRNA152-3p Protects Against Ischemia/Reperfusion-Induced Bbb Destruction Possibly Targeting the MAP3K2/JNK/c-Jun Pathway

Abstract

In the current study, we reported that overexpression of miR-152-3p effectively ameliorated neurological deficits and protected blood-brain barrier(BBB) integrity in middle cerebral artery occlusion (MCAO) rats. In an in vitro model, the level of miR-152-3p was significantly decreased in bEnd.3 cells after oxygen–glucose deprivation/reperfusion (OGD/R) insult. miR-152-3p overexpressing bEnd.3 cell monolayers were protected from OGD/R-induced microvascular hyperpermeability. The miR-152-3p-mediated protective effect was associated with lower apoptosis of endothelia by negatively modulating the MAP3K2/JNK/c-Jun pathway.

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

“…Overexpression of miR-126-3p/-5p and miR-98 in the ischemic brain of mice suppresses the effects of pro-inflammatory cytokines and adhesion molecules, preserving the integrity of the cerebral endothelium and thus reducing the negative consequences of stroke [252][253][254]. A similar effect is observed with overexpression of miR-152-3p, which reduces the degree of neurological deficit and disruption of BBB integrity in experimental ischemia by inhibiting apoptosis of endothelial cells through negative modulation of the MAP3K2/JNK/c-Jun pathway [255].…”