MicroRNA Responses to Cellular Stress

Marsit, Carmen J.; Eddy, Karen; Kelsey, Karl T.

doi:10.1158/0008-5472.can-06-1894

Cited by 363 publications

(242 citation statements)

References 41 publications

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“…In addition, miRNAs have also been suggested to be involved in the CSR; however, a few fundamental questions remain largely unanswered (Cimmino et al 2005;Leung et al 2006;Marsit et al 2006;Kulshreshtha et al 2007;Leung and Sharp 2007;Babar et al 2008). First, which specific miRNAs do cells express when exposed to stress?…”

Section: Discussionmentioning

confidence: 99%

“…In addition, recent studies suggest that miRNAs participate in the CSR (Cimmino et al 2005;Leung et al 2006;Marsit et al 2006;Kulshreshtha et al 2007;Leung and Sharp 2007;Babar et al 2008). However, several fundamental questions remain unanswered concerning the role that miRNAs may play in these mechanisms: First, which specific miRNAs are expressed by cells exposed to stress?…”

Section: Introductionmentioning

confidence: 99%

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Identification of microRNAs associated with hyperthermia-induced cellular stress response

Wilmink

Roth

Ibey

et al. 2010

Cell Stress and Chaperones

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MicroRNAs (miRNAs) are a class of small RNAs that play a critical role in the coordination of fundamental cellular processes. Recent studies suggest that miRNAs participate in the cellular stress response (CSR), but their specific involvement remains unclear. In this study, we identify a group of thermally regulated miRNAs (TRMs) that are associated with the CSR. Using miRNA microarrays, we show that dermal fibroblasts differentially express 123 miRNAs when exposed to hyperthermia. Interestingly, only 27 of these miRNAs are annotated in the current Sanger registry. We validated the expression of the annotated miRNAs using qPCR techniques, and we found that the qPCR and microarray data was in well agreement. Computational target-prediction studies revealed that putative targets for the TRMs are heat shock proteins and Argonaute-2-the core functional unit of RNA silencing. These results indicate that cells express a specific group of miRNAs when exposed to hyperthermia, and these miRNAs may function in the regulation of the CSR. Future studies will be conducted to determine if other cells lines differentially express these miRNAs when exposed to hyperthermia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs associated with hyperthermia-induced cellular stress response

Wilmink

Roth

Ibey

et al. 2010

Cell Stress and Chaperones

View full text Add to dashboard Cite

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“…Another study by Wang et al (2009) actually examined the effects of ethanol on in vivo mouse brain development and found that at least 14 different miRNAs were misexpressed. These included, in addition to miR-9 (as found by Sathyan et al, 2007), miR-10a and miR-200a (as in DKO cells) as well as miR-145 (as in FAD cells) (Han et al, 2007;Marsit et al, 2006). These embryos also displayed, amongst other abnormalities, defects in neural tube closure starting at concentrations of 2.0 mg/ml of ethanol.…”

Section: Mirna Misregulation and Ntdsmentioning

confidence: 92%

“…Although DHFR did not turn up any significant hits in the database search and miR-24 was not considered an miRNA of interest in this review (neither it is neural specific nor was it predicted to target Dll1, Dnmt1, Mthfr, or Rbl2), Mishra et al, 2004. showed that DHFR is targeted by miR-24, which was found to be upregulated 1.36 times greater than control in TK-6 cells grown in FAD media (Marsit et al, 2006). This suggests that folate deficiency could initiate a negative feedback loop where overexpression of miR-24 suppresses DHFR, which would further decrease methyl-donor pools, exacerbating methyl deficiency (Bohnsack and Hirschi, 2004;Pufulete et al, 2005;Fig.…”

Section: Dhfrmentioning

confidence: 96%

A new perspective on neural tube defects: Folic acid and microRNA misexpression

Shookhoff

Gallicano

2010

Genesis

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Summary: Neural tube defects (NTDs) are the second most common birth defects in the United States. It is well known that folic acid supplementation decreases about 70% of all NTDs, although the mechanism by which this occurs is still relatively unknown. The current theory is that folic acid deficiency ultimately leads to depletion of the methyl pool, leaving critical genes unmethylated, and, in turn, their improper expression leads to failure of normal neural tube development. Recently, new studies in human cell lines have shown that folic acid deficiency and DNA hypomethylation can lead to misexpression of microRNAs (miRNAs). Misexpression of critical miRNAs during neural development may lead to a subtle effect on neural gene regulation, causing the sometimes mild to severely debilitating range of phenotypes exhibited in NTDs. This review seeks to cohesively integrate current information regarding folic acid deficiency, methylation cycles, neural development, and miRNAs to propose a potential model of NTD formation. In addition, we have examined the relevant gene pathways and miRNAs that are predicted to affect them, and based on our investigation, we have devised a basic template of experiments for exploring the idea that miRNA misregulation may be linked to folic acid deficiency and NTDs. genesis 48:282-294, 2010. V V C 2010 Wiley-Liss, Inc.

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“…Arsenite, which is known to activate nuclear factor-erythroid 2-related factor 2 (Nrf2) (Aono et al, 2003), affects miRNA expression in human lymphoblastoid TH-6 cells (Marsit et al, 2006). Cigarette smoking causes the down-regulation of many miRNAs in the lungs of both mice and rats (Izzotti et al, 2011) as well as human airway epithelial cells (Schembri et al, 2009).…”

Section: Vreugdenhil Et Al (2009) Have Reported That Rat and Human Glmentioning

confidence: 99%

MicroRNAs from biology to future pharmacotherapy: Regulation of cytochrome P450s and nuclear receptors

Nakajima

Yokoi

2011

Pharmacology & Therapeutics

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MicroRNAs (miRNAs) are a family of short, non-coding RNA whose final product is a 22-nucleotide functional RNA molecule. They regulate the expression of target genes by binding to complementary regions of transcripts to repress their translation or promote mRNA degradation. Since miRNAs regulate every aspect of cellular function, their dysregulation is associated with a variety of diseases including cancer, diabetes, and cardiovascular diseases.Therefore, miRNAs are now considered new therapeutic targets. However, the roles of miRNAs in the metabolism of xenobiotics and endobiotics have only recently been revealed.This review describes the current knowledge on the regulation of cytochrome P450s and nuclear receptors by miRNAs, the physiological and clinical significance. The miRNA expression is readily altered by chemicals, carcinogens, drugs, hormones, stress, or diseases, and the dysregulation of specific miRNAs might lead to changes in the drug metabolism potency or pharmacokinetics as well as pathophysiological changes. In the field of pharmacogenomics, the evaluation of miRNA-related polymorphisms would provide useful information for personalized medicine. Utilizing miRNAs opens a new era in the fields of drug metabolism and pharmacokinetics as well as toxicology.

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MicroRNA Responses to Cellular Stress

Cited by 363 publications

References 41 publications

Identification of microRNAs associated with hyperthermia-induced cellular stress response

Identification of microRNAs associated with hyperthermia-induced cellular stress response

A new perspective on neural tube defects: Folic acid and microRNA misexpression

MicroRNAs from biology to future pharmacotherapy: Regulation of cytochrome P450s and nuclear receptors

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