Identification of microRNAs associated with hyperthermia-induced cellular stress response

Wilmink, Gerald J.; Roth, Caleb L.; Ibey, Bennett L.; Ketchum, Norma S.; Bernhard, Joshua A.; Cerna, Cesario Z.; Roach, William P.

doi:10.1007/s12192-010-0189-7

Cited by 55 publications

(68 citation statements)

References 61 publications

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“…As hyperthermia and oxidative stresses are the two major mechanisms for the varicocele-related spermatogenesis damage, we first selected five miRNAs (namely miR-15a, miR-16, miR-18a, miR-192, and miR-320) which were reported to be implicated in hyperthermia or oxidative cellular stresses , Ren et al 2009, Wilmink et al 2010. The expressions of these miRNAs in the ejaculated spermatozoa of ten pairs of patients with varicocele and the healthy donors were investigated by qRT-PCR.…”

Section: Expressions Of Stress-related Mirnas In the Ejaculated Spermmentioning

confidence: 99%

“…The miRNAs-mediated stress responses play important roles in plenty of human diseases, such as infection, cardiovascular disease, and cancer (Leung & Sharp 2010, Mendell & Olson 2012. Previous studies have shown that miR-15a, miR-16, miR-18, miR-192, and miR-320 are involved in hyperthermia or oxidative cellular stresses , Ren et al 2009, Wilmink et al 2010. However, the roles of miRNAs on varicocele-related stress environment remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Expressions of miR-15a and its target gene HSPA1B in the spermatozoa of patients with varicocele

Mou

et al. 2014

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Hyperthermia and oxidative stresses are the two central elements contributing to varicocele-related sperm damage. Growing evidence indicates that microRNAs (miRNAs) are involved in the regulation of the heat and oxidative stress responses. In this study, we analyzed the expressions of several stress-related miRNAs in the sperm and found that the expression of miR-15a was significantly decreased in patients with varicocele compared with the control. Furthermore, miR-15a repressed the expression of HSPA1B, which is a typical stressinduced chaperone protein, through directly binding its 3 0 -UTR. The expressions of miR-15a and HSPA1B exhibited an inverse correlation in sperm. Our results provide a valuable insight into the varicocele-related sperm impairment and male infertility, and may help to develop potential therapeutic targets and novel biomarkers for male infertility.

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Section: Expressions Of Stress-related Mirnas In the Ejaculated Spermmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expressions of miR-15a and its target gene HSPA1B in the spermatozoa of patients with varicocele

Mou

et al. 2014

Reproduction

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“…hsa-miR-34a, hsa-miR-125b, hsa-miR-128, hsa-miR-146a, hsa-miR-205, hsa-miR-221 and hsa-miR-432 were thermally upregulated whereas one miRNA (hsa-miR-154) was thermally downregulated [15] . Interestingly, hsa-miR-34a and hsa-miR-125b were earlier reported to be induced by heat shock in human dermal fibroblast [8] . The same study also reported heat shock-driven decreased hsa-miR-154 expression [8] , as also observed by us.…”

Section: Research Highlightmentioning

confidence: 98%

“…[6,7] . Over the years, active involvement of miRNAs in response to different stress stimuli including hyperthermia [8] , hypoxia [9] , ER stress [10] etc. has been reported.…”

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confidence: 99%

Widening HSF1 horizon: the microRNA connection

Das

Bhattacharyya

2015

RNA Dis

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Heat shock factor 1 (HSF1) is an evolutionary conserved transcription factor which acts as protector of cellular proteome against adverse environmental conditions including increased temperature in eukaryotes. HSF1 positively regulates expression of a set of cytoprotective proteins popularly called as heat shock proteins (HSPs) as part of an adaptive mechanism of cells known as heat shock response (HSR). It has also been shown that apart from cellular stress response, HSF1 has the ability to regulate many other biological processes including aging, metabolism, development etc. This is primarily achieved by the ability of HSF1 to regulate expression of myriad of genes other than those coding for classical HSPs in presence and/or absence of stress. MicroRNAs (miRNAs) are short endogenous RNA molecules which can regulate many biological processes by acting as post-transcriptional regulators of gene expression. Altered expression of miRNAs in response to thermal stress has been reported in different species; however, the underlying mechanism remained elusive. We recently showed that HSF1 has the ability to regulate expression of miRNAs by binding to their upstream sequences and our result established hsa-miR-432 as the first HSF1-regulated miRNA.We therefore speculate that the landscape of HSF1 transcripts is much broader than it was earlier thought and includes HSF1-regulated miRNAs, the recently identified non-coding arm of HSF1 transcripts. The HSF1-regulated miRNAs have the potential to regulate many cellular events by acting as important downstream molecules of HSF1.Our finding thus uncovers a novel functional aspect of HSF1 with the emergence of hsa-miR-432 as a novel transcriptional target of HSF1.

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“…[14][15][16] In comparison to scalpel incisions, those made by lasers in surgery generally have the disadvantage of causing a delay in wound healing due to collateral thermal damage to surrounding tissues. This was demonstrated in a study where differences in gene expression between free electron laser (FEL) and scalpel incisions in mouse skin were analyzed.…”

Section: Introductionmentioning

confidence: 99%

Image-guided genomic analysis of tissue response to laser-induced thermal stress

et al. 2011

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Abstract. The cytoprotective response to thermal injury is characterized by transcriptional activation of "heat shock proteins" (hsp) and proinflammatory proteins. Expression of these proteins may predict cellular survival. Microarray analyses were performed to identify spatially distinct gene expression patterns responding to thermal injury. Laser injury zones were identified by expression of a transgene reporter comprised of the 70 kD hsp gene and the firefly luciferase coding sequence. Zones included the laser spot, the surrounding region where hsp70-luc expression was increased, and a region adjacent to the surrounding region. A total of 145 genes were up-regulated in the laser irradiated region, while 69 were up-regulated in the adjacent region. At 7 hours the chemokine Cxcl3 was the highest expressed gene in the laser spot (24 fold) and adjacent region (32 fold). Chemokines were the most common up-regulated genes identified. Microarray gene expression was successfully validated using qRT-polymerase chain reaction for selected genes of interest. The early response genes are likely involved in cytoprotection and initiation of the healing response. Their regulatory elements will benefit creating the next generation reporter mice and controlling expression of therapeutic proteins. The identified genes serve as drug development targets that may prevent acute tissue damage and accelerate healing. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Identification of microRNAs associated with hyperthermia-induced cellular stress response

Cited by 55 publications

References 61 publications

Expressions of miR-15a and its target gene HSPA1B in the spermatozoa of patients with varicocele

Expressions of miR-15a and its target gene HSPA1B in the spermatozoa of patients with varicocele

Widening HSF1 horizon: the microRNA connection

Image-guided genomic analysis of tissue response to laser-induced thermal stress

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