MicroRNA profile analysis of host cells before and after wild human rotavirus infection

Abstract

Rotavirus infection is an important cause of acute gastroenteritis in children, but the interaction between rotavirus and host cells is not completely understood. We isolated a wildtype (wt) rotavirus strain, ZTR-68(P [8] G1), which is derived from an infant with diarrhea in southwest China in 2010. In this study, we investigated host cellular miRNA expression profiles changes in response to ZTR-68 in early stage of infection to investigate the role of miRNAs upon rotavirus infection. Differentially expressed miRNAs were identified by deep sequencing and qRT-PCR and the function of their targets predicted by Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. A total of 36 candidate miRNAs were identified. Comparative analysis indicated that 29 miRNAs were significantly down-regulated and 7 were up-regulated after infection. The data were provided contrasting the types of microRNAs in two different permissive cell lines (HT29 and MA104). The target assays results showed that mml-miR-7 and mml-miR-125a are involved in anti-rotavirus and virus-host interaction in host cells. These results offer clues for identifying potential candidates in vector-based antiviral strategies. J. Med. Virol. 88:1497-1510, 2016. © 2016 Wiley Periodicals, Inc.

MicroRNA-7 Inhibits Rotavirus Replication by Targeting Viral NSP5 In Vivo and In Vitro

“…There are few reports on the role of host microRNAs in antiviral defense and less is known about their mechanisms. In previous study [39], wild-type rotavirus ZTR-68 strain was isolated and used to infect the sensitive cell line MA104. Using deep RNA sequencing, the microRNA expression profile during rotavirus infection was analyzed.…”

“…In a previous study, we isolated the wild-type rotavirus ZTR-68 strain and allowed it to infect MA104 cells. We then performed deep RNA sequencing and analyzed the microRNA expression profile [39]. We found that the expression levels of 40 microRNAs were altered during rotavirus infection.…”

Immune and cytokine/chemokine responses of PBMCs in rotavirus‐infected rhesus infants and their significance in viral pathogenesis

“…The RV strain used in this study was the standard strain SA11 that was isolated from the monkeys. ZTR‐68 and SA11 were activated by 20 µg/mL trypsin for 1 hour at 37°C and cultured in MA104 cells in the presence of 1 µg/mL trypsin as previously described and harvested for freezing at −80°C.…”

Fecal MicroRNAs as Potential Biomarkers for Screening and Diagnosis of Intestinal Diseases

“…Repeated exposure to fecal pathogens, toxins, irritants, and pro-inflammatory cytokines can disrupt the tight junction’s barrier function, causing inflammation and facilitating the permeation of pro-inflammatory molecules such as unwanted colonial bacteria, bacterial antigens, and toxins to cross through and exacerbate the immune response ( Fukui, 2016 ; Hossain et al, 2016 ). In different studies, it has been shown that infections caused by enteric protozoa, rotavirus, astrovirus, enterovirus, adenovirus, and enterotoxigenic Escherichia coli have an association with the aberrant expression of host cellular miRNAs that has a profound impact on child growth and development ( Buret et al, 2002 ; Skalsky and Cullen, 2010 ; Zheng et al, 2013 ; Maudet et al, 2014 ; Ho et al, 2016 ; Zhou et al, 2016 ). miRNAs are highly relevant for infection-induced intestinal disorders as recent research has revealed their role in regulating the inflammatory responses and subsequently gastrointestinal diseases ( Sheedy and O’neill, 2008 ; Bi et al, 2009 ).…”