MicroRNA mir-34 provides robustness to environmental stress response via the DAF-16 network in C. elegans

Isik, Meltem; Blackwell, T. Keith; Berezikov, Eugène

doi:10.1038/srep36766

Cited by 50 publications

(40 citation statements)

References 75 publications

(85 reference statements)

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“…Our observation that these miRNAs increase in HS suggests that they may have roles in multiple stress response pathways. Down-regulation of miR-34 in response to our HS conditions was surprising given its reported roles in promoting dauer survival in response to food deprivation and ensuring normal development in animals subjected to rapid temperature fluctuations (61,62). However, miR-34 mutants have also been found to exhibit increased and decreased radiosensitivity in the soma and germline, respectively, suggesting that the function of this miRNA is highly context dependent (63).…”

Section: Specific Ncrnas From Multiple Classes Respond To Heat Shockmentioning

confidence: 90%

Remodeling of theC. elegansNon-coding RNA Transcriptome by Heat Shock

Schreiner¹,

Pagliuso²,

Garrigues³

et al. 2019

Preprint

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Elevated temperatures activate a Heat Shock Response (HSR) to protect cells from the pathological effects of protein mis-folding, cellular mis-organization, organelle dysfunction and altered membrane fluidity. This response includes activation of the conserved transcription factor Heat Shock Factor 1 (HSF-1), which binds Heat Shock Elements (HSEs) in the promoters of genes induced by heat shock (HS). The upregulation of protein-coding genes (PCGs), such as Heat Shock Proteins (HSPs) and cytoskeletal regulators, is critical for cellular survival during elevated temperatures. While the transcriptional response of PCGs to heat shock has been comprehensively analyzed in a variety of organisms, the effect of this stress on the expression of noncoding RNAs (ncRNAs) has not been systematically examined. Here we show that in Caenorhabditis elegans HS induces up-and down-regulation of specific ncRNAs from multiple classes, including miRNA, piRNA, lincRNA, pseudogene, and repeat elements. Moreover, some ncRNA genes appear to be direct targets of the HSR, as they contain HSF-1 bound HSEs in their promoters and their expression is regulated by this factor during HS. These results demonstrate that multiple ncRNA genes respond to HS, some as direct HSF-1 targets, providing new candidates that may contribute to organismal survival during this stress.

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Section: Specific Ncrnas From Multiple Classes Respond To Heat Shockmentioning

confidence: 90%

Remodeling of theC. elegansNon-coding RNA Transcriptome by Heat Shock

Schreiner¹,

Pagliuso²,

Garrigues³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In C. elegans, miR-34 is highly upregulated under temperature stress. miR-34 mutants subjected to the same stress have very low survival rates as compared with controls (Isik, Blackwell, & Berezikov, 2016). Zebrafish mutant for certain miR-NAs exhibit high variance in the development of the vascular system, with the incidence of defects increasing dramatically under temperature stress (Kasper et al, 2017).…”

Section: Small Rnasmentioning

confidence: 99%

Embryonic canalization and its limits—A view from temperature

Irvine

2020

J Exp Zool Pt B

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Many animals are able to produce similar offspring over a range of environmental conditions. This property of the developmental process has been termed canalization—the channeling of developmental pathways to generate a stable outcome despite varying conditions. Temperature is one environmental parameter that has fundamental effects on cell physiology and biochemistry, yet developmental programs generally result in a stable phenotype under a range of temperatures. On the other hand, there are typically upper and lower temperature limits beyond which the developmental program is unable to produce normal offspring. This review summarizes data on how development is affected by temperature, particularly high temperature, in various animal species. It also brings together information on potential cell biological and developmental genetic factors that may be responsible for developmental stability in varying temperatures, and likely critical mechanisms that break down at high temperature. Also reviewed are possible means for studying temperature effects on embryogenesis and how to determine which factors are most critical at the high‐temperature limits for normal development. Increased knowledge of these critical factors will point to the targets of selection under climate change, and more generally, how developmental robustness in varying environments is maintained.

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“…To determine a potential role for miRNAs in regulating gene expression in response to kairomone exposure, we took a targeted approach, monitoring the transcript levels of four miRNAs: miR-7, miR-34, miR-317, and miR-375. These miRNAs have each been proposed to function broadly during development by targeting cell cycle progression, as well as regulating stress response, immune response, morphological development and general metabolic pathways, respectively (Li et al, 2009, Isik et al, 2016, Jain et al 2015.…”

Section: Relative Mirna Expressionmentioning

confidence: 99%

Transcriptional response to kairomone exposure in Daphnia magna: A candidate gene approach

Telli¹,

Gordon²,

Ünlü³

2020

Preprint

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Background: Daphnia (Brachiopoda, Cladocera) is a well-studied model organism providing unparalleled opportunity to test epigenetic regulation of predator avoidance mechanisms in aquatic ecosystems. The discovery of regulatory functions for microRNA molecules and recently described miRNA profiles of Daphnia make it an ideal system to probe for posttranslational regulatory mechanisms mediated by kairomone released by predatory fish. However, despite a number of studies that focused on mRNA transcript level differences, no miRNA studies associated with kairomone exposure have been reported. Results: Exposing D. magna to fish kairomone from birth to the first reproduction was found to result in the differential expression of the four miRNAs tested: miR-7, miR-34, miR-317, and miR-375. Normalized transcript levels for each miRNA were found to vary across the exposure period with no clear conserved pattern of expression despite functional target analyses by GO, COG and KEGG indicating that predicted miRNA target genes are likely involved in related biological activities. Analysis of six mRNA transcripts (Hsp70, Hsp90, actin, AKT, GYS and IGFR), identified in previous studies as kairomone-mediated genes in Daphnia magna, were also carried out. Similar to that obtained for miRNAs, the mRNA transcript levels showed varying degrees of temporal regulation across the exposure time course with the two heat shock transcripts exhibiting elevated levels at early and late time points of kairomone exposure while the AKT, GYS, and IGFR transcripts had an general decrease in expression during the first 96 hours. Conclusions: Differential mRNA expression data supports the premise of an ecological trade-off between the cost of general biological processes and that of survival under long-term kairomone stress. Transcript levels for the four miRNAs tested were found to vary across developmental time with kairomone exposure which suggests that they may have a role in regulating morphological, behavioral or physiological responses by altering target gene expression. These studies lay the foundation for future work aimed at linking miRNAs and their target transcripts to changes in the signaling events that govern Daphnia response to kairomone specific stress.

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MicroRNA mir-34 provides robustness to environmental stress response via the DAF-16 network in C. elegans

Cited by 50 publications

References 75 publications

Remodeling of theC. elegansNon-coding RNA Transcriptome by Heat Shock

Remodeling of theC. elegansNon-coding RNA Transcriptome by Heat Shock

Embryonic canalization and its limits—A view from temperature

Transcriptional response to kairomone exposure in Daphnia magna: A candidate gene approach

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