MicroRNA-mediated signal amplification coupled with GNP/dendrimers on a mass-sensitive biosensor and its applications in intracellular microRNA quantification

Guo, Yingshu; Wang, Yujie; Yang, Guangxu; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1016/j.bios.2016.06.013

Cited by 24 publications

(7 citation statements)

References 34 publications

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“…Then, another miR‐27a solution and another PBS buffer was alternatively injected again several times but did not bring a significant frequency change. These results indicate that the hybridization of probe miRNA and complementary miR‐27a was effective and easy to be detected by QCM, the frequency change is comparable with those reported QCM sensors …”

Section: Resultssupporting

confidence: 85%

“…Then, another miR-27a solution and another PBS buffer was alternatively injected again several times but did not bring a significant frequency change. These results indicate that the hybridization of probe miRNA and complementary miR-27a was effective and easy to be detected by QCM, the frequency change is comparable with those reported QCM sensors 29,30. For single-base mismatch miR-27b, the frequency was decreased gradually and the frequency change reached to −24 Hz after 5000 seconds when the PBS buffer of 0.5 × 10 −6 M miR-27b was injected into , Schematic diagram of static detection quartz crystal microbalance sensor system.…”

supporting

confidence: 85%

“…Many studies have shown that DNA‐modified QCM biosensors can be applied to detect such processes as DNA hybridization and single base mismatch of DNA . However, studies on QCM biosensor for miRNA detection has been still limited . There are only several examples.…”

Section: Introductionmentioning

confidence: 99%

“…27 However, studies on QCM biosensor for miRNA detection has been still limited. [28][29][30] There are only several examples. In 2016, Guo et al developed a miRNA-bound QCM sensor coupled with gold nanoparticle (GNP)/dendrimerinduced signal enhancement for recognition of miRNA-203, which good linear relationship between frequency change and the concentration of miRNA-203 was shown in the range of 1.0 × 10 −11 -1.0 × 10 −9 M. 29 In 2017, Premaratne et al 30 reported a QCM biosensor for the detection of miRNA-21, and the detection limit (LOD) was 28 fM when combined with a signal enhancement strategy.…”

Section: Introductionmentioning

confidence: 99%

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Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

Zheng

et al. 2018

J of Molecular Recognition

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In this paper, a miRNA‐based quartz crystal microbalance (QCM) biosensor was fabricated and used to the rapid and effective sensing of miRNA. The specific hybridization between probe miRNA and different selected miRNAs (miR‐27a, miR‐27b, and Let‐7a) cause a different interaction mode, thus display different frequency change and response patterns in the QCM sensor, which were used to detect miR‐27a and miR‐27b. The selective sensing of miR‐27a in mixed miRNA solution was also achieved. This miRNA‐based QCM biosensor has the advantages of real‐time, label‐free, and short cycle detection.

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Section: Resultssupporting

confidence: 85%

supporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

Zheng

et al. 2018

J of Molecular Recognition

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“…The sensing method is to pick up on the frequency shift of QCM (quartz crystal microbalance) sensors (Piezoelectric biosensors concept). c. QCM sensors are compatible with different operating conditions (gaseous or liquid mediums) [26] making it a useful technique in health care. d. Gold-nanoparticles amplify the signal while coupled with an enzymatic amplification process.…”

Section: Recommendationsmentioning

confidence: 99%

Review on Gold Nanoparticles-Based Biosensors in Clinical and Non-Clinical Applications

Momeni

Javadifar

Patrick

et al. 2022

IJEMM

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Gold nanoparticles (GNP) acquire unique properties that have made significant contributions to clinical and non-clinical fields, specifically in the application of GNP’s for designing biosensor devices in which exhibit novel functional properties. Many properties of GNP’s are reviewed in this literature including optical properties, biocompatibility, conductivity, catalytic properties, high surface-to-volume ratio, and high density of the GNPs, that make them excellent in the application of constructing GNP-based biosensors. This literature review covers a specific comparison between the optical, electrochemical, and piezoelectric biosensors, as these are the three most common GNP-based biosensors. Optical biosensors are optimal due to their ability to cater to surface modification, which then leads to the ability for selective bonding. Furthermore, with the use of GNP and the sensor's non-invasive and non-toxic method of use, high-resolution images and signals can be formed. The sensitivity and specificity of electrochemical biosensors with the conductivity of GNPs, the electrodes of this stable biosensor can detect tumour markers in the human body. Piezoelectric biosensors are mass sensitive sensors and with the use of GNP, it amplifies the changes in mass. Through this, these sensors progress to be immunosensors which determine microorganisms and macromolecular compounds. As well, this review will conclude with an outline of present and future research recommendations for real-world application of the three GNP-based biosensors discussed.

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Advances in microRNA analysis

et al. 2017

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MicroRNAs (miRNAs) are single-stranded noncoding RNA molecules that act as key regulators of mRNA expression and are emerging biomarkers for disease. Their small size (18-25 nt) presents challenges to molecular recognition, labeling, and signal generation. Recent research activity in this field has aimed at the development of methods for miRNA quantification that combine high detectability, broad dynamic range, practicality, multiplexity, and low cost for prospective applications in diagnostic laboratories. This review article covers the most recent advances in microRNA analysis.

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MicroRNA-mediated signal amplification coupled with GNP/dendrimers on a mass-sensitive biosensor and its applications in intracellular microRNA quantification

Cited by 24 publications

References 34 publications

Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

Assembly of a miRNA‐modified QCM sensor for miRNA recognition through response patterns

Review on Gold Nanoparticles-Based Biosensors in Clinical and Non-Clinical Applications

Advances in microRNA analysis

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