MicroRNA-Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells

Sandmaier, Shelley E. S.; Telugu, Bhanu P.

doi:10.1007/978-1-4939-2848-4_3

Cited by 22 publications

(15 citation statements)

References 13 publications

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“…Additionally, the miR-302-3p-367 cluster has potential to convert somatic cells into induced pluripotent stem cells (26). A study by Cai et al (20) investigated the role of the miR-302-3p-367 cluster in cervical carcinoma.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Jiang

Hou

et al. 2018

Exp Ther Med

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Abstract. MicroRNA serve crucial roles in a variety of human cancer types. The miR-302-367 cluster has been reported to suppress the proliferation of cervical carcinoma cells through the novel target AKT1; however, the molecular mechanism of miR-302 in cervical cancer metastasis remains unclear. The present study aimed to investigate the clinical significance of miR-302-3p expression in cervical cancer, and to examine the regulatory mechanism of miR-302-3p in the malignant phenotypes of cervical cancer cells. The present data indicated that miR-302-3p was significantly downregulated in cervical cancer tissues compared with the level in adjacent non-tumor tissues, and low expression of miR-302-3p was significantly associated with node metastasis, advanced clinical stage, and poor prognosis in patients with cervical cancer. Restoration of miR-302-3p expression caused a significant reduction in cervical cancer cell migration and invasion. Defective in cullin neddylation 1 domain containing 1 (DCUN1D1) was identified as a novel target gene of miR-302-3p, and miR-302-3p negatively regulated the mRNA and protein expression of DCUN1D1 in cervical cancer HeLa cells. Additionally, overexpression of DCUN1D1 rescued the effects of miR-302-3p on the migration and invasion of cervical cancer cells. Furthermore, DCUN1D1 was upregulated in cervical cancer tissues compared with the levels in adjacent tissues, and its high expression was associated with node metastasis, advanced clinical stage, and shorter survival time in patients with cervical cancer. Notably, a negative correlation between miR-302-3p and DCUN1D1 expression in cervical cancer tissues was observed. Taken together, the present study suggests that miR-302-3p serves a suppressive role in cervical cancer metastasis, partly at least, via directly targeting DCUN1D1. Therefore, miR-302-3p/DCUN1D1 may be a potential therapeutic target for cervical cancer treatment.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Jiang

Hou

et al. 2018

Exp Ther Med

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“…The ESC-specific miR-302 family, which is composed of five members, miR-302a/b/c/d and miR-367, is highly expressed in undifferentiated mESCs, hESCs, and iPSCs, but suppressed in differentiated cells [ 98 ]. MiR-302 regulates the expression of the pluripotency markers Oct4, Sox2, Nanog, and SSEA-3/4 [ 99 ] and stimulates somatic cell reprogramming, which results in the generation of iPSCs through DNA demethylation and by downregulation of Dnmt1 [ 98 ] and methyl-DNA binding domain protein 2 (MBD2) [ 100 ]. In addition, miR-302 facilitates mesenchymal to epithelial transition or MET via targeting of the transforming growth factor β receptor II (TGFBR2) and the Ras homolog gene family member C genes [ 95 ].…”

Section: Importance Of Micrornas (Mirnas) Related To Induced Pluripotmentioning

confidence: 99%

Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency

et al. 2016

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Increasing evidence indicates that microRNAs (miRNAs), endogenous short non-coding RNAs 19–24 nucleotides in length, play key regulatory roles in various biological events at the post-transcriptional level. Embryonic stem cells (ESCs) represent a valuable tool for disease modeling, drug discovery, developmental studies, and potential cell-based therapies in regenerative medicine due to their unlimited self-renewal and pluripotency. Therefore, remarkable progress has been made in recent decades toward understanding the expression and functions of specific miRNAs in the establishment and maintenance of pluripotency. Here, we summarize the recent knowledge regarding the regulatory roles of miRNAs in self-renewal of pluripotent ESCs and during cellular reprogramming, as well as the potential role of miRNAs in two distinct pluripotent states (naïve and primed).

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“…These reprogramming miRs were identified upon a large analysis of miRs expression in mouse ESCs, mouse iPSCs, and adult mouse adipose stromal cells. Using this approach several mouse and human iPSCs lines have been derived [ 49 ]. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for regenerative medicine.…”

Section: Reprogramming Techniquesmentioning

confidence: 99%

An Overview of Direct Somatic Reprogramming: The Ins and Outs of iPSCs

Menon

Shailendra

Renda

et al. 2016

IJMS

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Stem cells are classified into embryonic stem cells and adult stem cells. An evolving alternative to conventional stem cell therapies is induced pluripotent stem cells (iPSCs), which have a multi-lineage potential comparable to conventionally acquired embryonic stem cells with the additional benefits of being less immunoreactive and avoiding many of the ethical concerns raised with the use of embryonic material. The ability to generate iPSCs from somatic cells provides tremendous promise for regenerative medicine. The breakthrough of iPSCs has raised the possibility that patient-specific iPSCs can provide autologous cells for cell therapy without the concern for immune rejection. iPSCs are also relevant tools for modeling human diseases and drugs screening. However, there are still several hurdles to overcome before iPSCs can be used for translational purposes. Here, we review the recent advances in somatic reprogramming and the challenges that must be overcome to move this strategy closer to clinical application.

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MicroRNA-Mediated Reprogramming of Somatic Cells into Induced Pluripotent Stem Cells

Cited by 22 publications

References 13 publications

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1

Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency

An Overview of Direct Somatic Reprogramming: The Ins and Outs of iPSCs

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