Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency

Lee, Young Jin; Ramakrishna, Suresh; Chauhan, Himanshu; Park, Won Sun; Hong, Seok Ho; Kim, Kye Seong

doi:10.1186/s13619-016-0028-0

Cited by 24 publications

(21 citation statements)

References 133 publications

(137 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The total ratio of the isomiR species versus their canonical miRNAs was relatively similar in all the LCC-lines, although the proportion of individual isomiRs sometime differed between the four LCC-derived EVs. In general, the isomiRs derived from the different cell lines were highly correlated with the canonical miRNAs present in the same EVs, supporting the [ 59 , 60 , 68 ] hypothesis that they would be likely to drive similar biology, similarly to what has already been suggested for cellular isomiRs [ 67 ].…”

Section: Discussionsupporting

confidence: 80%

RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines

et al. 2017

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 80%

RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This assumption is supported by the findings of Boellaard et al who recently documented the presence of miR-371a-3p in normal testicular tissue and its absence in other parts of the urogenital tract [27]. As miR-371 has been shown to be specifically associated with human stem cells [28][29][30] and as it is found in seminal plasma, too [31,32], it is rational to assume that this miR is specifically generated by normal testicular germ cells and most probably even more by the cells of GCT. In serum, patients with nonseminomas showed significantly higher miR-371a-3p expression, and CS2/3 patients had higher levels than those with CS1 [5].…”

Section: Mir-371a-3p Levels In the Tissues Of Tumor And Contralateralmentioning

confidence: 59%

Graded expression of microRNA-371a-3p in tumor tissues, contralateral testes, and in serum of patients with testicular germ cell tumor

et al. 2020

View full text Add to dashboard Cite

Copyright: Belge et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Background: Serum levels of microRNA-371a-3p represent a specific tumor marker of testicular germ cell tumors (GCTs) but the origin of circulating miR-371a-3p is not finally resolved. The correlation between miR-levels in tissue and serum is unclear.Results: MiR-levels in GCT tissue are 399-fold higher than in contralateral testicular tissue and 5843-fold higher than in non-testicular tissue. MiR tissue levels correlate with corresponding serum levels (r 2 = 0.181). ISH detected miR-371a-3p intracellularly in GCT cells except teratoma. A low expression was also detected in normal testicular germ cells.Conclusions: Circulating miR-371a-3p is specifically derived from GCT tissue. The miR is present in GCT cells except teratoma. A low expression is also found in normal testicular tissue but not in non-testicular tissue. MiR-371a-3p levels in tissue and serum correlate significantly. This study underscores the usefulness of serum miR-371a-3p as tumor marker of GCT.Patients and methods: Expression levels of miR-371a-3p were concurrently measured in tissues of GCT, contralateral testes (n = 38), and in serum (n = 36) with real time PCR. For control, 5 healthy testicles and 4 non-testicular tissue samples were examined. MiR-levels were compared using descriptive statistical methods. We also performed in situ hybridization (ISH) of GCT tissue with a probe specific for miR-371a-3p.

show abstract

“…MIR 302A is able to repress the key proteins such as cyclin D1and keep stem cell in G1 phase [ 71 ]. It has been suggested that MIR 302A is one of the key regulatory components conserved in mammalian pluripotent stem cells [ 72 – 74 ]. It has been documented that MIR 302A has the capability to reprogram human fibroblasts and human skin cancer to pluripotent stem cells [ 75 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells

et al. 2016

View full text Add to dashboard Cite

Human stem cells and progenitor cells can be used to treat cancer and replace dysfunctional cells within a tissue or organ. The objective of this study was to identify the appropriate cells type in regenerative medicine and targeted therapy. As an alternative to embryonic and bone marrow stem cells, we examined human amniotic fluid stem cells (hAFSCs), one of the potential source of multipotent stem cells isolated from both cell pellet (using single-stage method), and supernatant of human amniotic fluid. Source of isolation and unique property of the cells emphasize that these cells are one of the promising new tools in therapeutic field. Double sources for isolation and availability of the left over samples in diagnostic laboratory at the same time have less legal and ethical concerns compared with embryonic stem cell studies. Cells were isolated, cultured for 18th passage for 6 months and characterized using qPCR and flow cytometry. Cells showed good proliferative ability in culture condition. The cells successfully differentiated into the adipogenic and osteogenic lineages. Based on these findings, amniotic fluid can be considered as an appropriate and convenient source of human amniotic fluid stem cells. These cells provide potential tools for therapeutic applications in the field of regenerative medicine. To get a better understanding of crosstalk between Oct4/NANOG with osteogenesis and adipogenesis, we used network analysis based on Common Targets algorithm and Common Regulators algorithm as well as subnetwork discovery based on gene set enrichment. Network analysis highlighted the possible role of MIR 302A and MIR let-7g. We demonstrated the high expression of MIR 302A and low expression of MIR let7g in hAFSCs by qPCR.

show abstract

Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency

Cited by 24 publications

References 133 publications

RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines

RNA-seq reveals distinctive RNA profiles of small extracellular vesicles from different human liver cancer cell lines

Graded expression of microRNA-371a-3p in tumor tissues, contralateral testes, and in serum of patients with testicular germ cell tumor

Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells

Contact Info

Product

Resources

About