MicroRNA let-7c inhibits migration and invasion of human non-small cell lung cancer by targeting ITGB3 and MAP4K3

Zhao, Binghai; Han, Han; Chen, Jinfeng; Zhang, Zhiqian; Li, Shaolei; Fang, Fang; Zheng, Qingfeng; Ma, Yuanyuan; Zhang, Jianzhi; Wu, Nan; Yang, Yue

doi:10.1016/j.canlet.2013.08.030

Cited by 165 publications

(151 citation statements)

References 34 publications

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“…cell lung cancer (NSCLC), hsa-let-7c inhibits tumor metastasis and invasion via targeting the transcripts encoding integrin 3 and mitogen-activated protein kinase 3 (28). Previous studies have demonstrated that let-7 family members are involved in many carcinogenic pathways, including the Notch signaling pathway, which is associated with a number of diseases in humans (29,30).…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis

Huang¹,

Cong²,

Zhai³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Pneumonia is a lower respiratory tract infection that causes dramatic mortality worldwide. The present study aimed to investigate the pathogenesis of pneumonia and identify microRNA (miRNA) biomarkers as candidates for targeted therapy. RNA from the peripheral blood plasma of participants with pneumonia (severe, n=9; non-severe, n=9) and controls (n=9) was isolated and paired-end sequencing was performed on an Illumina HiSeq4000 system. Following the processing of raw reads, the sequences were aligned against the Genome Reference Consortium human genome assembly 38 reference genome using Bowtie2 software. Reads per kilobase of transcript per million mapped read values were obtained and the limma software package was used to identify differentially expressed miRNAs (DE-miRs). Then, DE-miR targets were predicted and subjected to enrichment analysis. In addition, a protein-protein interaction (PPI) network of the predicted targets was constructed. This analysis identified 11 key DE-miRs in pneumonia samples, including 6 upregulated miRNAs (including hsa-miR-34a and hsa-miR-455) and 5 downregulated miRNAs (including hsa-let-7f-1). All DE-miRs kept their upregulation/downregulation pattern in the control, non-severe pneumonia and severe pneumonia samples. Predicted target genes of DE-miRs in the subjects with non-severe pneumonia vs. the control and the subjects with severe pneumonia vs. the non-severe pneumonia group were markedly enriched in the adherens junction and Wnt signaling pathways. KALRN, Ras homolog family member A (RHOA), β-catenin (CTNNB1), RNA polymerase II subunit K (POLR2K) and amyloid precursor protein (APP) were determined to encode crucial proteins in the PPI network constructed. KALRN was predicted to be a target of hsa-mir-200b, while RHOA, CTNNB1, POLR2K and APP were predicted targets of hsa-let-7f-1. The results of the present study demonstrated that hsa-let-7f-1 may serve a role in the development of cancer and the Notch signaling pathway. Conversely, hsa-miR-455 may be an inhibitor of pneumonia pathogenesis. Furthermore, hsa-miR-200b might promote pneumonia via targeting KALRN.

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Section: Discussionmentioning

confidence: 99%

Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis

Huang¹,

Cong²,

Zhai³

et al. 2017

Experimental and Therapeutic Medicine

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“…A variety of studies have identified certain miRNAs that suppress the metastasis in lung cancer [9,10,21]. Let-7 is one of the earliest identified miRNAs with significantly reduced expression in a large number of malignant tumors and is known to attenuate the development of lung cancer [22,23]. As for miR-10b and miR-155, the upregulated expression level was significantly positively correlated with TNM stage, lymph node involvement, and poor survival [11,12].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

Xu¹,

Li²,

Li³

et al. 2015

Aging and disease

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Lymph node (LN) metastasis is often an early event in the progression of malignant tumors and it contributes to the majority of cancer mortalities. MiRNAs play key roles in tumor metastasis. This study aimed to investigate the specific miRNAs as putative indicators of metastasis early diagnosis for non-small cell lung cancer (NSCLC). In this study, five NSCLC cases with LN metastasis and four cases without metastasis (NLN) were enrolled for Agilent Human miRNA array. The interested differentially expressed miRNA was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the LN metastasis (n = 46) and NLN (n = 39) groups. The microarray results revealed that three miRNAs (miR-1260b, miR-423-3p, miR-23a-5p) were differentially expressed in LN metastasis group compared with NLN group. The expression of miR-1260b was tested by qRT-PCR and the mean relative expression fold change (2 -ΔΔCt ) in LN metastasis was significantly higher than that in the NLN group (3.942, 1.743 respectively, P = 1.179E-04). The patients with tumor-node-metastasis (TNM) stage III were identified more frequently in LN metastasis group (P = 1.772E-11) and with a higher expression level of miR-1260b (5.126, P = 1.147E-06). In addition, the LN metastasis cases were associated with a poorly differentiated degree (P = 0.007). The overexpression of miR1260b in NSCLC with LN metastasis can be regarded as a specific signature for early progression and prognosis of NSCLC.

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“…10,11 LncRNA-MALAT-1 was up-regulated during the tumourigenesis of lung cancer and demonstrated to be associated with metastasis in NSCLC patients. 12 Fas apoptotic inhibitory molecule 2 (FAIM2) could inhibit Fas-mediated cell death and was found to be overexpressed in SCLC cell lines.…”

mentioning

confidence: 99%

miR‐193b availability is antagonized by LncRNA‐SNHG7 for FAIM2‐induced tumour progression in non‐small cell lung cancer

et al. 2017

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Objectives: Long non-coding RNAs have identified to involve into the tumour cell proliferation, apoptosis and metastasis. We previously found that up-regulated LncRNA-SNHG7 (SNHG7) positively correlated to the Fas apoptosis inhibitory molecule 2 (FAIM2) in lung cancer cells with unclear mechanism.Methods: Non-small cell lung cancer (NSCLC) and relative normal tissues (n = 25) were collected. The SNHG7 expression and function in NSCLC was determined. The SNHG7-miR 193b-FAIM2 network was analysed in vitro and vivo.Results: We reported that oncogene SNHG7 predicted a poor clinical outcome and functioned as competitive endogenous RNA (ceRNA) antagonized microRNA-193b Conclusion:The results indicated that miR-193b is indispensible for the ceRNA role of SNHG7 in FAIM2-supported tumourigenesis of lung cancer.

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MicroRNA let-7c inhibits migration and invasion of human non-small cell lung cancer by targeting ITGB3 and MAP4K3

Cited by 165 publications

References 34 publications

Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis

Identification of miRNA biomarkers of pneumonia using RNA-sequencing and bioinformatics analysis

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

miR‐193b availability is antagonized by LncRNA‐SNHG7 for FAIM2‐induced tumour progression in non‐small cell lung cancer

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