MicroRNA Expression Profiles of Human Blood Monocyte-derived Dendritic Cells and Macrophages Reveal miR-511 as Putative Positive Regulator of Toll-like Receptor 4

Abstract

In conclusion, we have identified miR-511 as a novel potent modulator of human immune response. In addition, our data highlight that miRNA influence on gene expression is dependent on the cellular environment.

Regulation of Activation-associated MicroRNA Accumulation Rates during Monocyte-to-macrophage Differentiation

“…We then compared our results with a previous study that profiled miRNA expression in primary human CD14 MicroBead-purified monocytes differentiated to MDMs in the presence of GM-CSF (19). The most highly up-regulated miRNA in both MDM types in both the current study and the MDMs in the previous study (19) was miR-511. Additional miRNAs (miR-34a, miR-99b, miR-132, miR-146b-5p, miR-210, miR-212, and miR-449a) were also upregulated in both the current (Fig.…”

“…With the exception of miR-193b, the amplitude of change in the abundance of individual guide strands was less than 2-fold (14). In contrast to the small changes in miRNA expression described in our previous study, several studies have described robust induction of a subset of miRNAs in human monocytes responding to LPS and other inflammatory treatments (15)(16)(17)(18)(19). These observations led us to the important discovery that all activation-associated miRNAs tested had higher basal expression levels in MDMs than in monocytes (14).…”

“…Additional miRNAs (miR-34a, miR-99b, miR-132, miR-146b-5p, miR-210, miR-212, and miR-449a) were also upregulated in both the current (Fig. 6B) and the previous studies (19). In the case of miR-132 and miR-212, the regulation was enhanced by more than 4-fold when non-monocytic PBMCs were present during monocyte-to-macrophage differentiation, leading us to categorize this miRNA as being preferentially upregulated in the MDMs cultured in the presence of non-monocytic PBMCs (Fig.…”

“…Both Tserel et al (19) and Wang et al (22) described miR-150 as an miRNA with repressed expression during monocyteto-macrophage differentiation. We found that, although this miRNA was repressed during differentiation of purified monocyte cultures, the presence of non-monocytic PBMCs prevented the loss of expression for this miRNA in MDMs (Fig.…”

Functional regulation of monocyte-derived dendritic cells by microRNAs

“…Based on DC-SIGN/CD14 expression ratios, miR-21, miR-34a, and their cognate targets WNT1 and JAG1 were found to negatively influence moDC differentiation (Hashimi et al, 2009, Cekaite, 2010. Similarly, inhibition of miR-511 and miR-99b resulted in reduced DC-SIGN levels (Tserel et al, 2011). As being a versatile miRNA, miR-155 is also positively involved in maturation of mouse moDCs (Dunand-Sauthier et al, 2011;Lu et al, 2011a).…”