Regulation of Activation-associated MicroRNA Accumulation Rates during Monocyte-to-macrophage Differentiation

Eigsti, Renee L.; Sudan, Bayan; Wilson, Mary E.; Graff, Joel W.

doi:10.1074/jbc.m114.599316

Cited by 34 publications

(36 citation statements)

References 37 publications

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“…Down-regulation of miR-221/222 has also been implicated in the regulation of plaque neovascularization and monocyte differentiation to macrophages. 9, 13 While we focused our studies on the effects of loss of miR-221/222 in VSMCs, further studies examining the role of miR-221/222 in regulating plaque neovascularization and macrophage accumulation are therefore warranted.…”

Section: Discussionmentioning

confidence: 99%

Acute Loss of miR-221 and miR-222 in the Atherosclerotic Plaque Shoulder Accompanies Plaque Rupture

Bazan

Hatfield

O’Malley

et al. 2015

Stroke

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Background and Purpose Atherosclerotic plaque vulnerability is accompanied by changes in the molecular and cellular function in the plaque shoulder, including a decrease in vascular smooth muscle cell (VSMC) proliferation. We aimed to determine if the expression of three miRNAs that regulate VSMC proliferation (miR-145, miR-221, and miR-222) are altered with plaque rupture, suggesting a role in regulating plaque stability. Methods miRNAs were measured in the plaque shoulder of carotid plaques obtained from patients undergoing carotid endarterectomy (CEA) for three distinct clinical scenarios: 1) patients without prior neurologic events but high-grade carotid stenosis (asymptomatic), 2) patients with an acute neurologic event within 5 days of the CEA (urgent), and 3) patients undergoing CEA > 5 days after a neurologic event (symptomatic). Results Mean time from plaque rupture event to CEA was 2.4 days in the urgent group. The urgent group exhibited a significant decrease in miR-221 and miR-222 expression in the plaque shoulder while no significant differences were seen in miR-145 across the three groups. Regression analysis demonstrated a significant correlation between time from the neurologic event to CEA and increasing miR-221 and miR-222, but not miR-145. mRNA encoding p27Kip1, a target of miR-221 and -222 that inhibits VSMC proliferation, was increased in the urgent group. Conclusions Atherosclerotic plaque rupture is accompanied by a loss of miR-221 and -222 and an increase in p27Kip1 mRNA expression in the plaque shoulder, suggesting an association between these miRNAs and atherosclerotic plaque stability.

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Section: Discussionmentioning

confidence: 99%

Acute Loss of miR-221 and miR-222 in the Atherosclerotic Plaque Shoulder Accompanies Plaque Rupture

Bazan

Hatfield

O’Malley

et al. 2015

Stroke

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“…The underlying mechanism is that miR‐222 promotes the degradation of long noncoding RNA (lncRNA) growth arrest‐specific transcript 5 (GAS5), which is a CCL1 gene silencer . miR‐125a‐5p expression appears to be induced by LPS and inhibited by IFN‐γ and IL‐4 . Interestingly, inhibition of miR‐125a‐5p contributes to the weak expression of M2b macrophage markers, whereas miR‐125a‐5p overexpression enhances M2b polarization .…”

Section: Modulation Of M2b Macrophage Polarizationmentioning

confidence: 99%

“…49,103 miR-125a-5p expression appears to be induced by LPS and inhibited by IFN-and IL-4. 104,105 Interestingly, inhibition of miR-125a-5p contributes to the weak expression of M2b macrophage markers, whereas miR-125a-5p overexpression enhances M2b polarization. 104 In addition, miR-27a is increased but miR-26a-2 is decreased in M2b macrophages.…”

Section: Phenotypes Stimuli Markers Functions Referencesmentioning

confidence: 99%

M2b macrophage polarization and its roles in diseases

Wang

Zhang

et al. 2018

Journal of Leukocyte Biology

536

418

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Macrophages play an important role in a wide variety of physiologic and pathologic processes. Plasticity and functional polarization are hallmarks of macrophages. Macrophages commonly exist in two distinct subsets: classically activated macrophages (M1) and alternatively activated macrophages (M2). M2b, a subtype of M2 macrophages, has attracted increasing attention over the past decade due to its strong immune‐regulated and anti‐inflammatory effects. A wide variety of stimuli and multiple factors modulate M2b macrophage polarization in vitro and in vivo. M2b macrophages possess both protective and pathogenic roles in various diseases. Understanding the mechanisms of M2b macrophage activation and the modulation of their polarization might provide a great perspective for the design of novel therapeutic strategies. The purpose of this review is to discuss current knowledge of M2b macrophage polarization, the roles of M2b macrophages in a variety of diseases and the stimuli to modulate M2b macrophage polarization.

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“…The roles of miR‐146a in innate and adaptive immune processes have been reviewed in depth elsewhere (Labbaye & Testa, ; Testa, Pelosi, Castelli, & Labbaye, ). Many of these papers implicate miR‐146a‐mediated regulation of IRAK1, TRAF6, and NF‐κB signaling to be key in the immune/inflammatory cells within the TME, especially macrophages (Eigsti, Sudan, Wilson, & Graff, ; Hou et al, ; Taganov et al, ; Testa et al, ). In this exciting era of immuno‐oncology and immunotherapy, it would be interesting to examine miR‐146a in this regard.…”

Section: Mir‐146a In the Tumor Microenvironmentmentioning

confidence: 99%

miR‐146a‐5p: Expression, regulation, and functions in cancer

2019

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Cancer as we know it is actually an umbrella term for over 100 very unique malignancies in various tissues throughout the human body. Each type, and even subtype of cancer, has different genetic, epigenetic, and other cellular events responsible for malignant development and metastasis. Recent work has indicated that microRNAs (miRNAs) play a major role in these processes, sometimes by promoting cancer growth and other times by suppressing tumorigenesis. miRNAs are small, noncoding RNAs that negatively regulate expression of specific target genes. This review goes into an in‐depth look at the most recent finding regarding the significance of one particular miRNA, miR‐146a‐5p, and its involvement in cancer. Target gene validation and pathway analysis have provided mechanistic insight into this miRNA's purpose in assorted tissues. Additionally, this review outlines novel findings that suggest miR‐146a‐5p may be useful as a noninvasive biomarker and as a targeted therapeutic in several cancers. This article is categorized under: RNA in Disease and Development > RNA in Disease Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs

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Regulation of Activation-associated MicroRNA Accumulation Rates during Monocyte-to-macrophage Differentiation

Cited by 34 publications

References 37 publications

Acute Loss of miR-221 and miR-222 in the Atherosclerotic Plaque Shoulder Accompanies Plaque Rupture

Acute Loss of miR-221 and miR-222 in the Atherosclerotic Plaque Shoulder Accompanies Plaque Rupture

M2b macrophage polarization and its roles in diseases

miR‐146a‐5p: Expression, regulation, and functions in cancer

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