MicroRNA expression patterns in HbE/β-thalassemia patients: The passwords to unlock fetal hemoglobin expression in β-hemoglobinopathies

Das, Sankha Subhra; Das, Subhayan; Byram, Prasanna Kumar; Rahaman, Motiur; Dolai, Tuphan Kanti; Chatterjee, Anish; Chakravorty, Nishant

doi:10.1016/j.bcmd.2020.102523

Cited by 18 publications

(24 citation statements)

References 49 publications

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“…Differentially expressed miRNAs in adolescent or adult patients with β‐thalassemia have been reported in a previous study 46 . By integrating these datasets with our current results, we determined the unique and common miRNAs associated with pediatric and adult β‐thalassemia.…”

Section: Resultsmentioning

confidence: 56%

“…TGFβ is an important cytokine involved in cell migration 56,57 and negatively regulates erythrocyte differentiation and maturation in the early stages of erythropoiesis 58 . In addition, somatic stem cell population maintenance is associated with changes in genes in adult β‐thalassemia 46 …”

Section: Resultsmentioning

confidence: 99%

“…Because of the different expression profiles of pediatric and adult blood cells, 45 we hypothesized that pediatric and adult β‐thalassemia may have different molecular characteristics. Differentially expressed miRNAs in adolescent or adult patients with β‐thalassemia had been reported in a previous study 46 . However, the miRNA expression profiles in pediatric β‐thalassemia were unclear.…”

Section: Introductionmentioning

confidence: 80%

“…Differentially expressed miRNAs in adolescent or adult patients with β-thalassemia have been reported in a previous study. 46 By integrating these datasets with our current results, we determined the unique and common miRNAs associated with pediatric and adult β-thalassemia. Four miRNAs, that is, hsa-miR-29b-3p, hsa-miR-192-5p, hsa-miR-215-5p, and hsa-miR-150-5p, were upregulated in pediatric and adult patients with β-thalassemia (Figure 2A).…”

Section: Downregulation Of Let7 Mirnas In Pediatric and Adult Patients With β-Thalassemiamentioning

confidence: 99%

“…Differentially expressed miRNAs in adolescent or adult patients with β-thalassemia had been reported in a previous study. 46 However, the miRNA expression profiles in pediatric β-thalassemia were unclear. Accordingly, in this study, we determined the miRNA expression profiles modulated in pediatric patients with β-thalassemia.…”

Section: Introductionmentioning

confidence: 99%

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Abnormal regulation of microRNAs and related genes in pediatric β‐thalassemia

Wang

Chen

et al. 2021

Clinical Laboratory Analysis

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Background MicroRNAs (miRNAs) participate in the reactivation of γ‐globin expression in β‐thalassemia. However, the miRNA transcriptional profiles of pediatric β‐thalassemia remain unclear. Accordingly, in this study, we assessed miRNA expression in pediatric patients with β‐thalassemia. Methods Differentially expressed miRNAs in pediatric patients with β‐thalassemia were determined using microRNA sequencing. Results Hsa‐miR‐483‐3p, hsa‐let‐7f‐1‐3p, hsa‐let‐7a‐3p, hsa‐miR‐543, hsa‐miR‐433‐3p, hsa‐miR‐4435, hsa‐miR‐329‐3p, hsa‐miR‐92b‐5p, hsa‐miR‐6747‐3p and hsa‐miR‐495‐3p were significantly upregulated, whereas hsa‐miR‐4508, hsa‐miR‐20a‐5p, hsa‐let‐7b‐5p, hsa‐miR‐93‐5p, hsa‐let‐7i‐5p, hsa‐miR‐6501‐5p, hsa‐miR‐221‐3p, hsa‐let‐7g‐5p, hsa‐miR‐106a‐5p, and hsa‐miR‐17‐5p were significantly downregulated in pediatric patients with β‐thalassemia. After integrating our data with a previously published dataset, we found that hsa‐let‐7b‐5p and hsa‐let‐7i‐5p expression levels were also lower in adolescent or adult patients with β‐thalassemia. The predicted target genes of hsa‐let‐7b‐5p and hsa‐let‐7i‐5p were associated with the transforming growth factor β receptor, phosphatidylinositol 3‐kinase/AKT, FoxO, Hippo, and mitogen‐activated protein kinase signaling pathways. We also identified 12 target genes of hsa‐let‐7a‐3p and hsa‐let‐7f‐1‐3p and 21 target genes of hsa‐let‐7a‐3p and hsa‐let‐7f‐1‐3p, which were differentially expressed in patients with β‐thalassemia. Finally, we found that hsa‐miR‐190‐5p and hsa‐miR‐1278‐5p may regulate hemoglobin switching by modulation of the B‐cell lymphoma/leukemia 11A gene. Conclusion The results of the study show that several microRNAs are dysregulated in pediatric β‐thalassemia. Further, the results also indicate toward a critical role of let7 miRNAs in the pathogenesis of pediatric β‐thalassemia, which needs to be investigated further.

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Section: Resultsmentioning

confidence: 56%