MicroRNA dysregulation in adenoid cystic carcinoma of the salivary gland in relation to prognosis and gene fusion status: a cohort study

Abstract

Adenoid cystic carcinoma (ACC) is among the most frequent malignancies of the salivary gland, and is notorious for its prolonged clinical course characterized by frequent recurrences often years after initial treatment. No molecular marker has been shown to have independent prognostic value in ACC, including characteristic gene fusions involving MYB, MYBL1, and NFIB. MicroRNA has been shown to be associated with clinical outcome in numerous malignancies, including one study of ACC, warranting further validation of this class of markers in this disease. Here, we investigate the prognostic value of microRNA in two ACC cohorts: a training cohort (n = 64) and a validation cohort (n = 120) with microarray and qPCR. In the training cohort, multivariate analysis of microarray data found high expression of hsa-miR-6835-3p to be associated with reduced recurrence-free survival (RFS) (p = 0.016). Measuring the highest ranking microRNAs identified in survival analysis in the same cohort, qPCR identified high expression of hsa-miR-4676 to be associated with reduced overall survival (OS) and high expression of hsa-mir-1180 to be associated with improved RFS. This was not confirmed in the validation cohort, in which qPCR identified high expression of hsa-mir-21, hsa-mir-181a-2, and hsa-mir-152 to be associated with reduced OS and high expression of hsa-miR-374c to be associated with improved RFS. Interestingly, two distinct subsets of ACC separated in microRNA expression irrespective of gene fusion status, but without significant difference in outcome. Collectively, qPCR identified several microRNAs associated with OS and RFS, and different subsets of ACC separated according to microRNA expression, suggestive of ACC being a heterogeneous group of malignancies in its microRNA profile.

Lung metastasis in adenoid cystic carcinoma of the head and neck

“…But according to a review, the rate of detection of PNI varies widely from study to study . Most highly, there is a report that PNI was observed at a level of 82.1% with 184 patients . But as Vrielinck et al has already mentioned, when enough tissue is analyzed, detection of PNI in ACC is almost inevitable .…”

“…24 Most highly, there is a report that PNI was observed at a level of 82.1% with 184 patients. 27 But as Vrielinck et al has already mentioned, when enough tissue is analyzed, detection of PNI in ACC is almost inevitable. 28 In this regard, attention should be paid to interpret the significance of PNI as a prognostic factor.…”

Impact of lymph node sampling on survival in cN0 major salivary gland adenoid cystic carcinoma

“…However, several studies suggest that perineural invasion is not associated with survival. 21,22 Finally, the NCDB does not provide information on disease-specific mortality. However, the impact of comorbidity on survival was accounted for using Charlson/Deyo score.…”

“…The NCDB also does not provide information on perineural invasion, which has long been considered an important prognostic factor. However, several studies suggest that perineural invasion is not associated with survival . Finally, the NCDB does not provide information on disease‐specific mortality.…”

Clinical significance of miR-1180-3p in hepatocellular carcinoma: a study based on bioinformatics analysis and RT-qPCR validation

“…Indeed, the dysregulation of various miRNAs has been identified in malignant tumors and may serve as prognostic biomarkers and therapeutic targets 15 – 17 . In humans, miR-1180-3p is expressed from chromosome 17 (p11.2), and this miRNA may play different roles in carcinogenesis as drivers, oncogenes and tumor suppressors 18 – 20 . For example, Zhu et al 19 found that plasma miR-1180-3p is downregulated in early gastric cancer, suggesting that plasma miR-1180-3p can be used as new potential biomarker for the diagnosis of early gastric cancer.…”