MicroRNA-dependent regulation of PTEN after arsenic trioxide treatment in bladder cancer cell line T24

Cao, Yan; Yu, Song; Wang, Yan; Guo, Genmao; Ding, Qiang; An, Ruihua

doi:10.1007/s13277-010-0111-z

Cited by 74 publications

(32 citation statements)

References 26 publications

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“…[31] Knockdown of miR-19a also inhibited cell growth and induced cell apoptosis. [32] miR-19a is predicted to target the 3′ untranslated region of PTEN and BIM mRNA. Our study supports the notion that the levels of PTEN and BIM were downregulated in CM treated with Exo GATA-4 .…”

Section: Discussionmentioning

confidence: 99%

Exosomes secreted from GATA-4 overexpressing mesenchymal stem cells serve as a reservoir of anti-apoptotic microRNAs for cardioprotection

Kim

Gong

et al. 2015

International Journal of Cardiology

391

292

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Background Exosomes play an important role in intercellular signaling and exert regulatory function by carrying bioactive molecules. This study investigated the cardioprotective capabilities of exosomes derived from mesenchymal stem cells (MSC) overexpressing GATA-4 (MSCGATA-4) and its underlying mechanisms through delivering miroRNAs (miRs) to regulate the target proteins in recipient cells. Methods and Results Exosomes were isolated and purified from MSCGATA-4 (ExoGATA-4) and control MSCs (ExoNull). Cell injury was investigated in primary cultured rat neonatal cardiomyocytes (CM) and in the rat heart. Exosomes contributed to increased CM survival, reduced CM apoptosis, and preserved mitochondrial membrane potential in CM cultured under a hypoxic environment. Direct intramyocardial transplantation of exosomes at the border of an ischemic region following ligation of the left anterior descending coronary artery significantly restored cardiac contractile function and reduced infarct size. Real-time PCR revealed that several anti-apoptotic miRs were highly expressed in ExoGATA-4. Rapid internalization of ExoGATA-4 by CM was documented using time-lapse imaging. Subsequent expression of these miRs, particularly miR-19a was higher in CM and in the myocardium treated with ExoGATA-4 compared to those treated with ExoNull. The enhanced protective effects observed in CM were diminished by inhibition of miR-19a. The expression level of PTEN, a predicted target of miR-19a, was reduced in CM treated with ExoGATA-4, which resulted in the activation of the Akt and ERK signaling pathways. Conclusions ExoGATA-4 transferred miRs into damaged CM, triggering activation of the cell survival signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Exosomes secreted from GATA-4 overexpressing mesenchymal stem cells serve as a reservoir of anti-apoptotic microRNAs for cardioprotection

Kim

Gong

et al. 2015

International Journal of Cardiology

391

292

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“…Other miRNAs, such as anti-miR-21 and miR-15a/16-l, were reported to enhance the sensitivity of leukemic cells to the anti-cancer effect of As 2 O 3 and all-trans retinoic acid [23,24,25]. However, little attention has been paid to the role of miRNAs in the process of apoptosis induced by As 2 O 3 in APL patients.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs Contribute to Promyelocyte Apoptosis in As<sub>2</sub>O<sub>3</sub>-Treated APL Cells

Liang

Wang

et al. 2013

Cell Physiol Biochem

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Background: Arsenic trioxide (As₂O₃), an ancient drug used in traditional Chinese medicine, has substantial anticancer activities, especially in the treatment of patients suffering from acute promyelocytic leukemia (APL); however the underlying mechanisms are not well understood. Methods: MTT assay was used to detect the cell viability. Flow Cytometry analysis and caspase-3 activity assay were used to measure apoptosis of APL cells. Caspase-3 and Bax levels were analyzed by western blot and let-7d and miR-766 levels were determined by real-time RT-PCR. Results: As₂O₃ significantly inhibited cell viability and induced apoptosis in APL cells. Several microRNAs, including let-7d and miR-766, were dysregulated in APL cells treated with As₂O₃. The expression of caspase-3 and Bax, which are targets of let-7d and miR-766, respectively, were up-regulated in As₂O₃ treated cells. Transfection of let-7d and miR-766 into NB4 cells decreased the expression of caspase-3 and Bax, respectively. Correspondingly, transfection of these microRNAs increased NB4 cell viability. As₂O₃ induced degradation of promyelocytic leukemia (PML), and then induced the down-regulation of both let-7d and miR-766 in NB4 cells. Conclusions: We construct a dysregulated microRNA network involved in As₂O₃-induced apoptosis in APL. Targeting this network may be a new strategy for the prevention of side effects associated with APL treatment with As₂O₃.

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“…37 A similar study was conducted by Bollati et al regarding the influence of cadmium on miRNA-146a expression. 38,39 Bisphenol A is a compound that plays an essential role in the production of plastic masses used in the fast-food industry. Awissar-Whiting et al have reported changes in the expression of miRNA-21, miRNA-335, and miRNA-153 after interactions with this compound.…”

Section: Mirnas In Toxicologymentioning

confidence: 99%

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Dumache

Rogobete

Săndesc

et al. 2017

Journal of Interdisciplinary Medicine

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The current practice in the field of forensic medicine imposes the use of modern investigation techniques. The complexity of laboratory investigation methods needed for a final result of the investigation in forensic medicine needed new biomarkers of higher specificity and selectivity. Such biomarkers are the microRNAs (miRNAs), short, non-coding RNAs composed of 19-24 nucleotides. Their characteristics, such as high stability, selectivity, and specificity for biological fluids, differ from tissue to tissue and for certain pathologies, turning them into the ideal candidate for laboratory techniques used in forensic medicine. In this paper, we wish to highlight the biochemical properties and the usefulness of miRNAs in forensic medicine.

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MicroRNA-dependent regulation of PTEN after arsenic trioxide treatment in bladder cancer cell line T24

Cited by 74 publications

References 26 publications

Exosomes secreted from GATA-4 overexpressing mesenchymal stem cells serve as a reservoir of anti-apoptotic microRNAs for cardioprotection

Exosomes secreted from GATA-4 overexpressing mesenchymal stem cells serve as a reservoir of anti-apoptotic microRNAs for cardioprotection

MicroRNAs Contribute to Promyelocyte Apoptosis in As<sub>2</sub>O<sub>3</sub>-Treated APL Cells

Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

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