MicroRNA‐92b‐5p modulates melatonin‐mediated osteogenic differentiation of bone marrow mesenchymal stem cells by targeting ICAM‐1

Abstract

Osteoporosis is closely associated with the dysfunction of bone metabolism, which is caused by the imbalance between new bone formation and bone resorption. Osteogenic differentiation plays a vital role in maintaining the balance of bone microenvironment. The present study investigated whether melatonin participated in the osteogenic commitment of bone marrow mesenchymal stem cells (BMSCs) and further explored its underlying mechanisms. Our data showed that melatonin exhibited the capacity of regulating osteogenic differentiation of BMSCs, which was blocked by its membrane receptor inhibitor luzindole. Further study demonstrated that the expression of miR‐92b‐5p was up‐regulated in BMSCs after administration of melatonin, and transfection of miR‐92b‐5p accelerated osteogenesis of BMSCs. In contrast, silence of miR‐92b‐5p inhibited the osteogenesis of BMSCs. The increase in osteoblast differentiation of BMSCs caused by melatonin was attenuated by miR‐92b‐5p AMO as well. Luciferase reporter assay, real‐time qPCR analysis and western blot analysis confirmed that miR‐92b‐5p was involved in osteogenesis by directly targeting intracellular adhesion molecule‐1 (ICAM‐1). Melatonin improved the expression of miR‐92b‐5p, which could regulate the differentiation of BMSCs into osteoblasts by targeting ICAM‐1. This study provided novel methods for treating osteoporosis.

IL‐10 induces MC3T3‐E1 cells differentiation towards osteoblastic fate in murine model

“…IL-10 has profound and indispensable functional effects on infection, inflammation, tissue homeostasis, autoimmunity and cancer. [7][8][9][10][11] In recent years, the IL-10 family have been shown to regulate arthritis, 12,13 suggesting potential effects on osteoblast differentiation. Similarly, a recent study had reported that overexpression of IL-10 could disrupt osteoclast differentiation via NF-ĸB signalling.…”

Immunosuppressive Property of MSCs Mediated by Cell Surface Receptors

“…In the inflammatory environment, the surface receptor may be more critical than the paracrine effect of MSCs, because the inflammatory cytokine enhances the surface receptors expression and promote MSCs immigrate to the target site and exert immunosuppression effect in different stage. Those studies attracted our interests and promoted us to identify specific cell surface molecules expressed on ADSCs (42,43). These surface molecules, including chemokine and chemotactic receptors, adhesion molecule cytokine and cytokine receptors, co-inhibitory molecules, and other surface markers, have been proven to be related to the immunosuppression properties of tumor cells.…”

MicroRNA‐23 suppresses osteogenic differentiation of human bone marrow mesenchymal stem cells by targeting the MEF2C‐mediated MAPK signaling pathway

“…Human bone marrow mesenchymal stem cells (hBMSCs) were the first cells to be discovered in the mesenchymal stem cell family. 1,2 hBMSCs can be induced in a specific environment to differentiate into a variety of tissue cells, including osteoblasts, 3 adipocytes, 4 chondrocytes 5 and nerve cells. 6 Han et al 7 8 Numerous studies have confirmed that miRNAs can indirectly regulate the proliferation, differentiation and biological behaviors of stem cells, including hBMSCs.…”